Abstract: A method of constructing humanized murine model using stem cells, includes obtaining human stem cells; transplanting human stem cells into murine with liver damage; and obtaining hepatotropic virus infected humanized murine. It was found that by inducing severe liver damage and transplanting human stem cells, human-derived hepatocytes in murine liver have a high chimeric rate of 50-95%, and human-derived immune cells may exist in murine organs such as spleen, blood, liver, and bone marrow, thereby forming murine model of humanized liver and immune cell. The humanized murines are then infected with various types of hepatotropic viruses to form humanized hepatotropic viral infected murine model. In addition to construction of model for studying hepatotropic viral infection using the technique for constructing the humanized murine model, the concept of this technical solution may also be used for constructing models of other humanized organs.

Abstract: A humanized murine model of chronic hepatitis B constructed using human stem cells, obtained by obtaining the human stem cells; transplanting the human stem cells into a murine with liver damage to achieve presence of 1×104-8 of the human stem cells in the murine in vivo; etc. The humanized murine model may be used for the study of drug resistance to the antiviral drugs, the study of the pathogenesis of chronic hepatitis B, etc. In addition to the study of the mechanism of hepatitis B viral infection, the humanized murine model may also be used for research in treatment.