Abstract: The present disclosure provides a Bacillus subtilis strain for production of lycopene by an extracellular organelle. Specifically, in the present disclosure, synthesized crtE, crtI and crtB genes are integrated into plasmid PADK using gene recombination technology to construct plasmid PADK-FQHS, and the plasmid is transformed into Bacillus subtilis to construct strain BS PADK-FQHS. Subsequently, a dyna gene derived from Bacillus subtilis is integrated into plasmid PHT01 using gene recombination technology to construct plasmid PHT-dyna, and the plasmid is transformed into the strain BS PADK-FQHS to construct strain BS PADK-FQHS PHT-dyna. The lycopene yields in strains BS PADK-FQHS and BS PADK-FQHS PHT-dyna are detected, which demonstrates the promoting effect of nanotubes on the production of lycopene by Bacillus subtilis, with the yield increased by 67.1%.