Abstract: Methods and compositions for characterizing a redox reagent system enzyme are provided. In practicing the subject methods, a sample that includes a redox reagent system enzyme and a known amount of substrate is applied to an electrochemical cell that includes an enzyme-free reagent composition having a redox reagent system mediator. Also provided are electrochemical test strips that include the subject electrochemical cells, and systems and kits that include the same. The subject invention finds use in a variety of different applications, including redox reagent system characterization applications.

Abstract: Multilayer reagent test strips for quantitating glycated protein in a fluid sample, as well as methods for using the same, are provided. The subject multilayer test strips include at least a filter layer, a proteinase layer and a ketoamine oxidase signal producing and fluid flow control system layer. In using the subject test strips, a fluid sample is applied to the test strip and a signal is generated that can be employed to quantitate the glycated protein level in the sample. The quantitated glycated protein level can then be employed to determine the amount of glycated protein in the fluid sample. Also provided are kits and systems that include the subject test strips and find use in practicing the subject methods. The subject compositions and methods find use in glycated protein monitoring applications, among other utilities.

Abstract: Multilayer reagent test strips for quantitating glycated protein in a fluid sample, as well as methods for using the same, are provided. The subject multilayer test strips include at least a filter layer, a proteinase layer and a ketoamine oxidase signal producing and fluid flow control system layer. In using the subject test strips, a fluid sample is applied to the test strip and a signal is generated that can be employed to quantitate the glycated protein level in the sample. The quantitated glycated protein level can then be employed to determine the amount of glycated protein in the fluid sample. Also provided are kits and systems that include the subject test strips and find use in practicing the subject methods. The subject compositions and methods find use in glycated protein monitoring applications, among other utilities.

Abstract: An immunoassay cassette, apparatus, and method for detecting an analyte in a liquid body-fluid sample are disclosed. The cassette has a body and a support mounted on the body, for movement relative to the body to first and second transfer positions. Sample supplied to a sample well in the cassette body is transferred to a reagent reservoir on the cassette body, by moving the support to its first transfer position. Here the sample reacts with a first reagent composition in the reservoir effective to form a modified sample. The modified sample is then transferred to a reagent strip on the support by moving the support to its second transfer position. The reagent strip has a transfer zone at which sample material is transferred to the strip, a second reagent composition effective to react with the modified sample to form a detectable analyte-dependent product, and a detection zone located downstream at which the detectable product can be observed.

Abstract: Chromatographic strip is used in combination with a transmittance detecting system in a test to quantitate analytes in biological fluid. Chemical reagents or conjugate labels are simply absorbed on the passages' materials of the strip. The substrates, affinity reagents, or antibodies are immobilized on transparent beads in the detection cell of the strip. The biological fluid passes through the strip. The captured analytes are detected by transmittance detection for quantification. Uncaptured elements and interferences in the fluid are drained to the absorbent portion of the strip when the fluid passes the cell as a wash. This chromatographic strip with an analyte capture zone simplifies the. procedures that a transmittance detecting system alone cannot overcome. Adjustable light path of the cells in the strip overcome the sensitivity limitation of reflectance detection.

Abstract: Multilayer reagent test strips for quantitating glycated protein in a fluid sample, as well as methods for using the same, are provided. The subject multilayer test strips include at least a filter layer, a proteinase layer and a ketoamine oxidase signal producing and fluid flow control system layer. In using the subject test strips, a fluid sample is applied to the test strip and a signal is generated that can be employed to quantitate the glycated protein level in the sample. The quantitated glycated protein level can then be employed to determine the amount of glycated protein in the fluid sample. Also provided are kits and systems that include the subject test strips and find use in practicing the subject methods. The subject compositions and methods find use in glycated protein monitoring applications, among other utilities.

Abstract: Multilayer reagent test strips that include at least one hydrophobic fluid flow delay layer, as well as methods for using the same, are provided. The hydrophobic fluid flow delay layer of the subject test strips is one that has been treated with an non-polar organic solvent to provide for a layer which delays fluid flow through a multilayer reagent test strip in a reproducible manner. Also provided are kits and systems that include the subject test strips and find use in practicing the subject methods. The subject compositions and methods find use in a variety of different analyte detection applications.