Patents by Inventor Suzanne M. Otani

Suzanne M. Otani has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
    Publication number: 20080213901
    Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less of the trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated trichodiene synthases and isolated nucleic acid sequences encoding the trichodiene synthases.
    Type: Application
    Filed: January 31, 2008
    Publication date: September 4, 2008
    Applicant: Novozymes, Inc.
    Inventors: John C. Royer, Lynne M. Christianson, Gregory A. Gambetta, Howard Brody, Suzanne M. Otani, Wendy T. Yoder
  • Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
    Patent number: 7332341
    Abstract: The present invention relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells.
    Type: Grant
    Filed: March 31, 2005
    Date of Patent: February 19, 2008
    Assignee: Novozymes, Inc.
    Inventors: John C. Royer, Lynne M. Christanson, Gregory A. Gambetta, Howard Brody, Suzanne M. Otani, Wendy T. Yoder
  • Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
    Patent number: 6903193
    Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells, isolated trichodiene synthases and nucleic acid sequences encoding the trichodiene synthases.
    Type: Grant
    Filed: November 10, 2000
    Date of Patent: June 7, 2005
    Assignee: Novozymes Biotech, Inc.
    Inventors: John C. Royer, Lynne M. Christianson, Gregory A. Gambetta, Howard Brody, Suzanne M. Otani, Wendy T. Yoder
  • Method for preparing polypeptide variants
    Publication number: 20030199038
    Abstract: The present invention relates to methods for preparing variants of a nucleotide sequence, comprising: (a) introducing into a population of filamentous fungal host cells: (i) one or more circular plasmids comprising a DNA sequence and a plasmid replicator mediating autonomous replication, wherein the one or more circularized plasmids are linearized by digestion of the DNA sequence and removal of a portion of the DNA sequence; and (ii) a library of DNA fragments comprising one or more mutations of the DNA sequence, wherein the fragments comprise at least two regions, one or more regions which are homologous to the 5′ region or the 3′ region of the gap in the linearized DNA sequence and/or plasmid sequence and one or more second regions which are homologous to the 5′ region or the 3′ region of the DNA fragments of the library; wherein the linearized plasmids and the DNA fragments recombine by in vivo recombination to produce a plurality of autonomously replicating plasmids comprising one
    Type: Application
    Filed: April 22, 2003
    Publication date: October 23, 2003
    Applicant: Novozymes Biotech, Inc.
    Inventors: Howard Brody, Suzanne M. Otani, Joel R. Cherry
  • Methods for producing heterologous polypeptides in trichothecene-deficient
    Patent number: 6180366
    Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less of the trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated trichodiene synthases and isolated nucleic acid sequences encoding the trichodiene synthases.
    Type: Grant
    Filed: May 20, 1999
    Date of Patent: January 30, 2001
    Assignee: Novo Nordisk Biotech, Inc.
    Inventors: John C. Royer, Lynne M. Christianson, Gregory A. Gambetta, Howard Brody, Suzanne M. Otani, Wendy T. Yoder