Abstract: The present invention relates to a composition for use in hybridization, the composition comprising: (a) at least one hybridization probe (“component (a)”); (b) at least one polar protic or polar aprotic solvent (“component (b)”) having a cyclic molecule structure; and (c) at least one carboxylic acid amide and/or salts thereof (“component (c)”), in an amount of more than 10 vol %, based on the composition. The invention furthermore relates to a use of this composition, and to methods for detecting nucleic acids and/or chromosomal aberrations in a biological sample by way of hybridization, using the composition.

Abstract: The invention relates to a method for identifying chromosomal abnormalities, particularly structural and/or numerical chromosomal abnormalities, and preferably structural chromosomal abnormalities, using insitu hybridization by detecting chromosomes and/or DNA regions in a biological sample, preferably in one or more cell(s) and/or in one or more cell nuclei.

Abstract: The invention relates to a method for identifying chromosomal abnormalities, particularly structural and/or numerical chromosomal abnormalities, and preferably structural chromosomal abnormalities, using in situ hybridization by detecting chromosomes and/ or DNA regions in a biological sample, preferably in one or more cell(s) and/or in one or more cell nuclei.

Abstract: The invention relates to a method for identifying chromosomal abnormalities, particularly structural and/or numerical chromosomal abnormalities, and preferably structural chromosomal abnormalities, using insitu hybridization by detecting chromosomes and/or DNA regions in a biological sample, preferably in one or more cell(s) and/or in one or more cell nuclei.

Abstract: The present invention relates to a composition for use in hybridization, the composition comprising: (a) at least one hybridization probe (“component (a)”); (b) at least one polar protic or polar aprotic solvent (“component (b)”) having a cyclic molecule structure; and (c) at least one carboxylic acid amide and/or salts thereof (“component (c)”), in an amount of more than 10 vol %, based on the composition. The invention furthermore relates to a use of this composition, and to methods for detecting nucleic acids and/or chromosomal aberrations in a biological sample by way of hybridization, using the composition.

Abstract: The invention relates to a method for detecting several different chromosomes or DNA regions in a cell in order to provide evidence for structural chromosomal aberrations, wherein the chromosomal aberrations have at least two breaking point regions within a chromosome, on the basis of directly or indirectly labeled nucleic acid fragments (probes), wherein: a first probe labeled with label A (probe A) and a second probe labeled with label B (probe B) flank a breaking point region 1, and form the fusion signals A-B; and two probes, a third and a fourth, each labeled with a label C (probes C), flank a breaking point region 2, and form the fusion signals C-C, wherein the above-mentioned fusion signals change in the event of a chromosomal aberration to fusion signals A-C and to fusion signals B-C.

Abstract: The invention relates to a method for detecting several different chromosomes or DNA regions in a cell in order to provide evidence for structural chromosomal aberrations, wherein the chromosomal aberrations have at least two breaking point regions within a chromosome, on the basis of directly or indirectly labeled nucleic acid fragments (probes), wherein: a first probe labeled with label A (probe A) and a second probe labeled with label B (probe B) flank a breaking point region 1, and form the fusion signals A-B; and two probes, a third and a fourth, each labeled with a label C (probes C), flank a breaking point region 2, and form the fusion signals C-C, wherein the above-mentioned fusion signals change in the event of a chromosomal aberration to fusion signals A-C and to fusion signals B-C.