Abstract: Methods and compositions are provided for engineering microorganisms, which permit enhanced H2 production. The methods and compositions provided include novel chimeric gene constructs encoding H2-forming H2ase and maturation proteins, allowing for generation of H2 continuously in large quantities. In one illustrated embodiment, novel engineered algae are provided with increased levels of H2 production.

Abstract: Methods and compositions are provided for engineering microorganisms, which permit enhanced H2 production. The methods and compositions provided include novel chimeric gene constructs encoding H2-forming H2ase and maturation proteins, allowing for generation of H2 continuously in large quantities. In one illustrated embodiment, novel engineered algae are provided with increased levels of H2 production.

Abstract: A broad-range plant defense system employing thionins is disclosed. The invention protects various crop species and other plant species from attack by a wide range of phytopathogens, providing broad resistance to diverse plant diseases, using transgenes encoding a seed-derived thionin and a suitable signal peptide. Resistant crop varieties can not only prevent yield losses due to bacterial and fungal diseases, but can also expand the geographic boundaries of possible growing areas for economically important crops.