Abstract: Provided herein are methods, compositions, systems, and kits for recombination assays, many of which involve amplification reactions such as PCR or droplet digital PCR.

Abstract: The present invention provides methods of detecting a target molecule in a sample comprising incubating the sample with two or more detectably labeled probes, partitioning the sample into multiple partitions, and detecting the presence of the two or more probes in the same partition.

Abstract: Methods compositions and kits are provided for performing a chromatin or chromosome conformation capture assay in partitions.

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable directional chromosome mapping e.g., using chromosome phasing/haplotyping. The improved methods, compositions, and kits can also enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g., whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

Abstract: Methods compositions and kits are provided for performing a chromatin or chromosome conformation capture assay in partitions.

Abstract: Methods and compositions are provided for detection and quantification of nucleic acid sequences.

Abstract: Provided herein are methods, compositions, systems, and kits for recombination assays, many of which involve amplification reactions such as PCR or droplet digital PCR.

Abstract: The present invention provides methods of detecting a target molecule in a sample comprising incubating the sample with two or more detectably labeled probes, partitioning the sample into multiple partitions, and detecting the presence of the two or more probes in the same partition.

Abstract: This disclosure provides methods for measuring the copy number for highly amplified and/or abundant genomic loci. Recognized herein is a need for methods for determining nucleic acid copy number, particularly in instances where one locus to be quantified (i.e., the target) is relatively more abundant than a locus of known abundance (i.e., the reference). In some cases, the method involves combining a query nucleic acid sample with a diluting nucleic acid sample and measuring the relative copy number of a target sequence compared with a reference sequence in the combined sample.

Abstract: The present disclosure provides a digital assay system, includes methods and apparatus, with reduced measurement uncertainty. In an exemplary method, an expected value for a measure that is a function of a level of a first target and a level of a second target in a sample may be provided. An optimal concentration for the first target may be obtained based on the expected value. An experimental value for the measure may be determined from a digital assay with partitions formed according to the optimal concentration.