Abstract: Provided herein are methods and systems for production (e.g., batch production) of a polypeptide product via cell culture. In some embodiments, the methods and systems use a first bioreactor (e.g., for cell culturing), an alternating tangential flow (ATF) microfilter (e.g., for removing a polypeptide product and culture medium from the cell culture while retaining cells), a second bioreactor (e.g., for concentrating the product), and an ATF ultrafilter (e.g., for retaining product in the second bioreactor and allowing culture medium to exit).

Abstract: The invention provides methods for preparing antibodies and antibody derivatives with controlled levels of core fucosylation. In one aspect, provided herein is a method of controlling the level of afucosylation of an antibody or antibody derivative. In some embodiments, the invention provides a composition of antibodies or antibody derivatives produced by the instant methods. The antibodies and derivatives can be formulated as pharmaceutical compositions comprising a therapeutically or prophylactically effective amount of the antibody or derivative and one or more pharmaceutically acceptable ingredients.

Abstract: The present invention provides a method for removing cysteine caps from antibodies and re-capping the antibodies with cysteine molecules. The methods include, inter alia, culturing a host cell comprising a protein molecule having at least one capped engineered cysteine residue, and contacting the cell culture with cystine. Dissolved oxygen levels can be manipulated in the cell culture to further enhance the removal and re-capping process.

Abstract: The present invention provides a method for removing cysteine caps from antibodies and re-capping the antibodies with cysteine molecules. The methods include, inter alia, culturing a host cell comprising a protein molecule having at least one capped engineered cysteine residue, and contacting the cell culture with cystine. Dissolved oxygen levels can be manipulated in the cell culture to further enhance the removal and re-capping process.

Abstract: The invention relates to an improved process of producing a fermentation product, such as ethanol, from starch-containing material using an increased dosage of alpha-amylase during liquefaction, which results in increased conversion of dextrins compared to conventional processes, and/or a reduced dosage of carbohydrate-generating enzyme during simultaneous saccharification and fermentation compared to a conventional process carried out at the same conditions. A process of the invention results in an increased yield. The invention also relates to an improved process of producing a fermentation product resulting in substantially the same yield as a conventional process, wherein reduced dosage of carbohydrate-source generating enzyme is used during simultaneous saccharification and fermentation.

Abstract: A process for the production of a valuable compound, comprising the steps of a) fermentation of a filamentous bacterial or fungal strain (e.g. a Streptomyces strain or an Aspergillus strain) in a fermentation medium wherein a carbohydrate during fermentation is added in a cyclic pulse dosing/pause way, wherein the pulse dosing time is shorter than the pause time and b) recovery of the valuable compound from the fermentation broth.

Abstract: The invention relates to a process of producing a fermentation product, such as ethanol, from starch-containing material, including i) subjecting starch-containing material to an alpha-amylase, ii) subjecting the material obtained in step i) to an alpha-glucosidase and/or a maltose-generating enzyme, and iii) fermenting the material in the presence of a fermenting organism, such as yeast. Alternatively the invention relates to a process of producing a fermentation product from starch-containing material, preferably granular starch, which process comprises: a) subjecting starch-containing material to an alpha-glucosidase and optionally a glucose-generating and/or maltose-generating enzyme, and b) fermenting the material in the presence of a fermenting organism.

Abstract: The present invention relates to method of liquefying starch-containing material, wherein the method is carried out in stages. The invention also relates to a process of producing ethanol comprising a liquefaction step carried out according to the liquefaction method of the invention.

Abstract: The present invention relates to a process for liquefying starch-containing material, comprising treating the starch-containing material with a bacterial alpha-amylase at a temperature between 70-90° C. for 10-120 minutes and a pullulanase at a temperature in the range from 40-60° C. for between 20 and 90 minutes. The invention also relates to a saccharification process for saccharifying liquefied starch-containing material, comprising saccharifying a liquefied starch-containing material in the presence of a carbohydrate-source generating enzyme and a pullulanase. Finally, the invention also relates to a process of producing a fermentation product, such as ethanol, comprising a liquefaction step and/or saccharification step carried out in accordance with the present invention.

Abstract: The invention relates to a process of producing a fermentation product, such as ethanol, from starch-containing material, including i) subjecting starch-containing material to an alpha-amylase, ii) subjecting the material obtained in step i) to an alpha-glucosidase and/or a maltose-generating enzyme, and iii) fermenting the material in the presence of a fermenting organism, such as yeast. Alternatively the invention relates to a process of producing a fermentation product from starch-containing material, preferably granular starch, which process comprises: a) subjecting starch-containing material to an alpha-glucosidase and optionally a glucose-generating and/or maltose-generating enzyme, and b) fermenting the material in the presence of a fermenting organism.

Abstract: The present invention relates to method of liquefying starch-containing material, wherein starch-containing material is subjected to a bacterial alpha-amylase at (a) a temperature around 50-80° C. for 10-180 minutes, followed by (b) treatment at a higher temperature than used in step (a) for 1-60 minutes.

Abstract: The present invention relates to a process of liquefying starch-containing material, with a bacterial alpha-amylase at a temperature in the range from 65-75° C. for 1 to 2 hours. The invention also relates to a process of producing a fermentation product, preferably ethanol, using the liquefaction process of the invention.

Abstract: The present invention relates to method of liquefying starch-containing material, wherein the method comprises the steps of (a) treating the starch-containing material with a bacterial alpha-amylase at a temperature around 70-90° C. for 15-90 minutes, and (b) treating the material obtained in step (a) with an alpha-amylase at a temperature between 60-80° C. for 30-90 minutes. The invention also relates to a process of producing a fermentation product, preferably ethanol, comprising a liquefaction step carried out according to the liquefaction method of the invention.

Abstract: A process for the production of a valuable compound, comprising the steps of a) fermentation of a filamentous bacterial or fungal strain (e.g. a Streptomyces strain or an Aspergillus strain) in a fermentation medium wherein a carbohydrate during fermentation is added in a cyclic pulse dosing/pause way, wherein the pulse dosing time is shorter than the pause time and b) recovery of the valuable compound from the fermentation broth.