Abstract: Methods, systems, and apparatus, including computer programs encoded on computer storage media, for generating customized recommendations for environmentally-conscious cloud computing frameworks for replacing computing resources of existing datacenters. One of the methods involves receiving, through a user interface presented on a display of a computing device, data regarding a user's existing datacenter deployment and the user's preferences for the new cloud computing framework, generating one or more recommendations for environmentally-conscious cloud computing frameworks based on the received data, and presenting such recommendations through the user interface for the user's review and consideration.

Abstract: Aspects of the invention described herein concern the incorporation of a FOXP3 cDNA (e.g., full-length human codon-optimized cDNA) into a FOXP3 gene or a non-FOXP3 locus so as to provide constitutive or regulated FOXP3 expression in a primary human CD34+ cells or cells derived from edited CD34+ cells. In some embodiments, guide RNA sequences that are directed to FOXP3, AAVS1, or other candidate loci are used for CRISPR/Cas9-mediated gene regulation, and gene delivery cassettes for HDR based gene-modification are provided.

Abstract: Aspects of the invention described herein concern the incorporation of a FOXP3 cDNA (e.g., full-length human codon-optimized cDNA) into a FOXP3 gene or a non-FOXP3 locus so as to provide constitutive or regulated FOXP3 expression in a primary human CD34+ cells or cells derived from edited CD34+ cells. In some embodiments, guide RNA sequences that are directed to FOXP3, AAVS1, or other candidate loci are used for CRISPR/Cas9-mediated gene regulation, and gene delivery cassettes for HDR based gene-modification are provided.

Abstract: Aspects of the invention described herein concern the incorporation of a FOXP3 cDNA (e.g., full-length human codon-optimized cDNA) into a FOXP3 gene or a non-FOXP3 locus so as to provide constitutive or regulated FOXP3 expression in a primary human CD34+ cells or cells derived from edited CD34+ cells. In some embodiments, guide RNA sequences that are directed to FOXP3, AAVS1, or other candidate loci are used for CRISPR/Cas9-mediated gene regulation, and gene delivery cassettes for HDR based gene-modification are provided.

Abstract: Described herein are compositions and methods for treating, inhibiting or ameliorating X linked agammaglobulinemia (XLA) in subjects that have been identified or selected as being ones that would benefit from a therapy to treat, inhibit, or ameliorate XLA. Exemplary embodiments include constructs and methods for gene therapy, which restore or increase BTK expression.

Abstract: Various embodiments of the present technology generally relate to systems and methods for self-healing services and automatic recovery of distribute systems. Some embodiments of the present technology leverage all the available synthetic, customer, client, server, support signals from various sources to intelligently and in real-time detect outages, root cause outages to recoverable targets (e.g., for auto recovery actions), identify the right engineering teams (e.g., for faster manual mitigation), and perform the appropriate recovery action (such as recycle service, reboot server, switch out a faulty rack) or other mitigation actions such as routing, collecting debug information, alerting to the right team, or alert suppression. Some embodiments separate signal monitoring and workflow coordination.

Abstract: Various embodiments of the present technology generally relate to systems and methods for self-healing services and automatic recovery of distribute systems. Some embodiments of the present technology leverage all the available synthetic, customer, client, server, support signals from various sources to intelligently and in real-time detect outages, root cause outages to recoverable targets (e.g., for auto recovery actions), identify the right engineering teams (e.g., for faster manual mitigation), and perform the appropriate recovery action (such as recycle service, reboot server, switch out a faulty rack) or other mitigation actions such as routing, collecting debug information, alerting to the right team, or alert suppression. Some embodiments separate signal monitoring and workflow coordination.