Abstract: Disclosed herein is a microfluidic device comprising, at least one sample inlet for receiving biological cells in a biological fluid sample; at least one sheath flow inlet for receiving a sheath fluid; at least one curvilinear channel configured to provide the biological fluid sample substantially in an outer flow and the sheath fluid in substantially an inner separated flow; a plurality of cell traps at the periphery of the curvilinear channel, each trap configured to admit a single cell having a targeted size range from the outer flow.

Abstract: Disclosed herein is a microfluidic device comprising, at least one sample inlet for receiving biological cells in a biological fluid sample; at least one sheath flow inlet for receiving a sheath fluid; at least one curvilinear channel configured to provide the biological fluid sample substantially in an outer flow and the sheath fluid in substantially an inner separated flow; a plurality of cell traps at the periphery of the curvilinear channel, each trap configured to admit a single cell having a targeted size range from the outer flow.

Abstract: A micro-fluidic device operable to isolate target cells from a biological fluid comprises: an inlet operable to receive the biological fluid, the biological fluid comprising target cells and other components; a waste outlet operable to receive at least the other components of the biological fluid; a plurality of parallel arrays of cell isolation wells coupling the inlet with the waste outlet, each parallel array of cell isolation wells supporting a flow of the biological fluid from the inlet to the waste outlet in response to a pressure differential thereacross, each array of cell isolation wells comprising a plurality isolation wells, each isolation well being dimensioned to mechanically trap the target cells therein whilst permitting flow of other components of the biological fluid; and at least one pressure maintenance structure operable to assist in maintaining a predetermined pressure differential across each of the plurality of parallel arrays of cell isolation wells.

Abstract: In accordance with an embodiment of the invention, there is provided a microfluidic device for isolating cells from a biological fluid. The device comprises an inlet receiving the biological fluid flowed into the device, and at least one array of a plurality of isolation wells receiving the biological fluid from the inlet. At least one isolation well of the plurality of isolation wells comprises a cell trap of a size and shape suitable to mechanically isolate a cell within the cell trap. The cell trap comprises at least one gap of a size and shape suitable to prevent passage of the cells to be isolated but to permit passage of other components of the biological fluid through the cell trap.