Abstract: Certain aspects of the present invention are directed to a method for making a protein blocking assembly reversibly linking a therapeutic protein to a blocking group via a linker moiety. Additional aspects of the invention are directed to the protein blocking assembly, and to methods of administering the protein blocking assembly to a subject, where the protein is cleaved from the protein blocking assembly.

Abstract: The present invention is directed to a composition suitable for forming an implanted light activated drug depot, methods of making the composition, and methods and systems for using the composition. The composition comprises a plurality of drug conjugates, which comprise a drug molecule and a small solubility modulating portion. The drug conjugates are insoluble upon implantation as a drug depot into a subject, and the drug is preferably soluble once cleaved from the depot. One aspect of the invention is directed to a drug conjugate having a modulating portion that modifies the solubility of the drug conjugate by employing a hydrophobic non-polar moiety. Another aspect of the invention is directed to a drug conjugate having a modulating portion that modifies the solubility of the drug by employing a charged moiety that shifts the isoelectric point of the drug conjugate to a physiological pH.