Abstract: The present disclosure relates to a novel CRISPR-Cas9 based system for editing mitochondrial DNA. Aspects of the disclosure provide for mitochondrial translocation of both the guide RNA and the recombinant Cas9 nuclease.

Abstract: A method and composition for modifying bentonite to allow the bentonite to be useful in drilling mud applications. The method includes the steps of: preparing bentonite local to Saudi Arabia using raw water to remove contaminants from the bentonite; grinding the bentonite to a fine powder; sieving the fine powder to be between about 50 ?m and about 150 ?m in particle size to produce a sieved fine powder; mixing the sieved fine powder with polyanionic cellulose polymer to produce a modified bentonite composition; adding the modified bentonite composition to water until a homogeneous solution of modified bentonite in water is formed; and allowing the homogeneous solution of modified bentonite in water to rest for about 16 hours to form a composition useful in drilling mud applications.

Abstract: The present disclosure relates to a novel CRISPR-Cas9 based system for editing mitochondrial DNA. Aspects of the disclosure provide for mitochondrial translocation of both the guide RNA and the recombinant Cas9 nuclease.

Abstract: A method and composition for modifying bentonite to allow the bentonite to be useful in drilling mud applications. The method includes the steps of: preparing bentonite local to Saudi Arabia using raw water to remove contaminants from the bentonite; grinding the bentonite to a fine powder; sieving the fine powder to be between about 50 ?m and about 150 ?m in particle size to produce a sieved fine powder; mixing the sieved fine powder with polyanionic cellulose polymer to produce a modified bentonite composition; adding the modified bentonite composition to water until a homogeneous solution of modified bentonite in water is formed; and allowing the homogeneous solution of modified bentonite in water to rest for about 16 hours to form a composition useful in drilling mud applications.

Abstract: Disclosed is a method for modifying bentonite to allow the bentonite to be useful in drilling mud applications. The method includes the steps of: preparing bentonite local to Saudi Arabia using raw water to remove contaminants from the bentonite; grinding the bentonite to a fine powder; sieving the fine powder to be between about 50 ?m and about 150 ?m in particle size to produce a sieved fine powder; mixing the sieved fine powder with polyanionic cellulose polymer to produce a modified bentonite composition; adding the modified bentonite composition to water until a homogeneous solution of modified bentonite in water is formed; and allowing the homogeneous solution of modified bentonite in water to rest for about 16 hours to form a composition useful in drilling mud applications.

Abstract: Disclosed is a method for modifying bentonite to allow the bentonite to be useful in drilling mud applications. The method includes the steps of: preparing bentonite local to Saudi Arabia using raw water to remove contaminants from the bentonite; grinding the bentonite to a fine powder; sieving the fine powder to be between about 50 ?m and about 150 ?m in particle size to produce a sieved fine powder; mixing the sieved fine powder with polyanionic cellulose polymer to produce a modified bentonite composition; adding the modified bentonite composition to water until a homogeneous solution of modified bentonite in water is formed; and allowing the homogeneous solution of modified bentonite in water to rest for about 16 hours to form a composition useful in drilling mud applications.

Abstract: A plastic lab bottle created by an injection blow molding process comprises the steps of injecting plastic into a first mold from the plastic lab bottle bottom to form a preformed plastic lab bottle; blowing the preformed plastic lab bottle into a second mold by injecting air into the preformed plastic lab bottle through the mouth opening of the preformed plastic lab bottle; ejecting the finished plastic container from the second mold; providing a plastic cap for closing the bottle; and pulling the tether ring over the neck ring of the bottle.