Abstract: The present invention includes an immunogenic protein, nucleic acid, plant and immunization comprising a fusion protein that has at least 90% amino acid identity to an amino acid sequence of a modified thermostable lichenase (LicKM) polypeptide as set forth in SEQ ID NO:9, wherein the LicKM polypeptide comprises an N-terminus, a C-terminus, and an inner loop region, and wherein a Receptor Binding Domain (RBD) or a Receptor Binding Motif (RBM) of a coronavirus spike protein is positioned at, at least one of, the N-terminus, the C-terminus, or in a loop region of the LicKM polypeptide.

Abstract: The present invention includes compositions and methods of reducing the glycosylation of proteins comprising: obtaining a cell that expresses one or more proteins that comprise one or more glycosylation sites and are glycosylated; expressing in the cell one or more glycosidases that cleaves one or more glycosyl groups from the one or more proteins; and isolating the one or more proteins with reduced glycosylation from the cell.

Abstract: The present invention includes compositions and methods of reducing the glycosylation of proteins comprising: obtaining a cell that expresses one or more proteins that comprise one or more glycosylation sites and are glycosylated; expressing in the cell one or more glycosidases that cleaves one or more glycosyl groups from the one or more proteins; and isolating the one or more proteins with reduced glycosylation from the cell.