Abstract: An apparatus for performing nanoparticle-assisted external beam radiotherapy includes an X-ray spectrometer having an optical axis, an X-ray filter, and a mobile holding structure for holding the X-ray filter and X-ray spectrometer in a first and second relative position, and for switching from the first to second relative position while simultaneously positioning a patient body part in a target region including a target point. An X-ray beam emitted from the X-ray source crosses the X-ray filter before reaching the target point. The propagation of the X-ray beam forms an angle different from 0° and 180° with the optical axis of the X-ray spectrometer. The second relative position is obtained by inverting the relative positions of the X-ray filter and X-ray spectrometer relative to the patient body part. A method of determining an X-ray dose delivered at a region of a patient body using the apparatus is provided.

Abstract: The present invention relates to the use of nanodiamonds as drugs generating free radicals, in particular for treating tumors. The invention is based on generating free radicals on the surface of the nanodiamonds when they are exposed to radiation, for example ionizing radiation. In order to increase the effectiveness of the nanodiamonds, the nanodiamonds can be complexed with a radiosensitizing agent, such as a chemical molecule or an interfering RNA targeting a repairing gene.

Abstract: An apparatus for performing nanoparticle-assisted external beam radiotherapy includes an X-ray spectrometer having an optical axis, an X-ray filter, and a mobile holding structure suitable for holding the X-ray filter and the X-ray spectrometer in a first and a second relative position, and for switching from the first to the second relative position while simultaneously allowing the positioning of a patient body part in a target region including a target point; wherein: the first and second relative positions of the X-ray filter and the X-ray spectrometer are such that an X-ray beam emitted from the X-ray source crosses the X-ray filter before reaching the target point, a propagation direction of the X-ray beam forming an angle ? different from 0° and 180° with the optical axis of the X-ray spectrometer; and the second relative position is obtained by inverting the relative positions of the X-ray filter and of the X-ray spectrometer with respect to the patient body part.

Abstract: A process, for the quantitative analysis by optical emission spectroscopy of a plasma induced by a laser beam of at least one target included in a biochip, involves the use of an adjuvant allowing the formation of a dry matrix capable of being ablated simultaneously with the at least one target, the dry matrix being configured to improve the analytical properties of plasma, the adjuvant having an emission spectrum whose lines have wavelengths distinct from the wavelengths of the spectral lines used for the quantitative analysis of said at least one target.

Abstract: The invention relates to a method for evaluating, via biodosimetry, the irradiation dose received by a person subjected to ionizing radiation. Said method comprises: a) sampling, in at least one area of the body of the person, the follicles or bulbs of head and/or body hair of the person; b) extracting proteins from cells of said sampled hair bulbs or follicles, wherein said proteins, including proteins from the ATM system, are subjected to phosphorylation and/or acetylation induced by ionizing radiation; and c) analyzing at least two types of extracted proteins and interpreting the analysis results in order to determine the irradiation dose in the or each sampling area.

Abstract: A process, for the quantitative analysis by optical emission spectroscopy of a plasma induced by a laser beam of at least one target included in a biochip, involves the use of an adjuvant allowing the formation of a dry matrix capable of being ablated simultaneously with the at least one target, the dry matrix being configured to improve the analytical properties of plasma, the adjuvant having an emission spectrum whose lines have wavelengths distinct from the wavelengths of the spectral lines used for the quantitative analysis of said at least one target.

Abstract: The present invention relates to the use of nanodiamonds as drugs generating free radicals, in particular for treating tumours. The invention is based on generating free radicals on the surface of the nanodiamonds when they are exposed to radiation, for example ionising radiation. In order to increase the effectiveness of the nanodiamonds, the nanodiamonds can be complexed with a radiosensitising agent, such as a chemical molecule or an interfering RNA targeting a repairing gene.

Abstract: The present invention relates to a device and method for the high speed quantitative measurement of biomolecular targets on the surface or in the body of a planar medium for biological analysis.

Abstract: The present invention relates in particular to a method for the quantitative measurement of biomolecular targets that have been deposited on a biochip (1) of the type with a matrix of probes hybridized by the targets, the matrix comprising a multitude of measurement points (2) each comprising a plurality of probes, characterized in that it comprises the following steps: a) at least one laser beam (18) is focused onto each measurement point, in order to extract therefrom a hot confined plasma comprising a chemical element to be quantified that is present in the targets and optionally in the probes; b) the light emission lines from the plasma are detected and analysed for each measurement point, by measuring the respective intensities of these lines; and then c) the concentration in each measurement point of the element or of a group incorporating it within the targets is determined via a prior calibration of the lines establishing a correlation between the intensities of the lines specific to the element to be

Abstract: Novel chimeric molecules, termed “half-antibodies”, which are capable of self-assembling to form an epitope recognition site. Using these half-antibodies or a vesicle, a viral particle, a composition or a kit thereof, for therapeutic applications, such as the prevention or treatment of cancers, genetic diseases, infectious diseases, and for in vitro diagnostic applications and detecting biological molecules. The half-antibodies include at least two chimeric molecules A and B, each has a polypeptide domain characteristic of a variable domain of a heavy chain or of a light chain of an antibody, and a nucleotide domain, the nucleotide domain of A and that of B being capable of pairing into a double stranded structure. Biologically active nucleic sequences can be grafted onto these chimeric molecules to prevent the expression of target genes in the interior of a human or non-human mammalian cell.

Abstract: The invention relates to a method of analysing molecular targets contained in a complex mixture, comprising the following steps consisting in: a) bringing the mixture of molecular targets to be analysed into contact with an array of different types of primary probes, whereby each type of primary probe forming the array can bind specifically to a type of target selected from among the molecular targets, under conditions that enable specific binding between the molecular targets and the primary probes; b) optionally eliminating the primary probes that are not bound specifically to a molecular target; c) separating the molecular targets and the primary probes which are bound specifically in a probe/target complex, such as to recover the array of primary probes representing a fingerprint of the molecular targets to be analysed; and d) quantitatively analysing the primary probes eluted in step c.

Abstract: The invention relates to a method of analysing molecular targets contained in a complex mixture, comprising the following steps consisting in: a) bringing the mixture of molecular targets to be analysed into contact with an array of different types of primary probes, whereby each type of primary probe forming the array can bind specifically to a type of target selected from among the molecular targets, under conditions that enable specific binding between the molecular targets and the primary probes; b) optionally eliminating the primary probes that are not bound specifically to a molecular target; c) separating the molecular targets and the primary probes which are bound specifically in a probe/target complex, such as to recover the array of primary probes representing a fingerprint of the molecular targets to be analysed; and d) quantitatively analysing the primary probes eluted in step c.

Abstract: The invention relates to a device for separating and/analyzing several molecular targets dissolved in a complex mixture which is characterized in that it comprises a) a matrix of micro-columns, wherein each micro-column (2) comprises an immobilized molecular probe for retaining a specific molecular target contained in the complex mixture by specific probe/target linkage, b) a first capillary network (3) for circulating the complex mixture introduced into the inventive device towards each micro-column of the matrix defined in a), c) a second capillary network (4) for circulating, after elution, the molecular targets retained on the micro-columns towards a sensor (5) for carrying out the recovery and/or analysis thereof, and d) if necessary, a sensor (5), preferably in the form of a mass spectrometer, for carrying out the recovery and/or analysis of different molecular targets.

Abstract: The present invention relates to a device and method for the high speed quantitative measurement of biomolecular targets on the surface or in the body of a planar medium for biological analysis.

Abstract: The invention relates to a device for separating and/analyzing several molecular targets dissolved in a complex mixture which is characterized in that it comprises a) a matrix of micro-columns, wherein each micro-column (2) comprises an immobilized molecular probe for retaining a specific molecular target contained in the complex mixture by specific probe/target linkage, b) a first capillary network (3) for circulating the complex mixture introduced into the inventive device towards each micro-column of the matrix defined in a), c) a second capillary network (4) for circulating, after elution, the molecular targets retained on the micro-columns towards a sensor (5) for carrying out the recovery and/or analysis thereof, and d) if necessary, a sensor (5), preferably in the form of a mass spectrometer, for carrying out the recovery and/or analysis of different molecular targets.

Abstract: The invention relates to a device for separating and/analyzing several molecular targets dissolved in a complex mixture which is characterized in that it comprises a) a matrix of micro-columns, wherein each micro-column (2) comprises an immobilized molecular probe for retaining a specific molecular target contained in the complex mixture by specific probe/target linkage, b) a first capillary network (3) for circulating the complex mixture introduced into the inventive device towards each micro-column of the matrix defined in a), c) a second capillary network (4) for circulating, after elution, the molecular targets retained on the micro-columns towards a sensor (5) for carrying out the recovery and/or analysis thereof, and d) if necessary, a sensor (5), preferably in the form of a mass spectrometer, for carrying out the recovery and/or analysis of different molecular targets.

Abstract: The present invention relates in particular to a method for the quantitative measurement of biomolecular targets that have been deposited on a biochip (1) of the type with a matrix of probes hybridized by the targets, the matrix comprising a multitude of measurement points (2) each comprising a plurality of probes, characterized in that it comprises the following steps: a) at least one laser beam (18) is focused onto each measurement point, in order to extract therefrom a hot confined plasma comprising a chemical element to be quantified that is present in the targets and optionally in the probes; b) the light emission lines from the plasma are detected and analysed for each measurement point, by measuring the respective intensities of these lines; and then c) the concentration in each measurement point of the element or of a group incorporating it within the targets is determined via a prior calibration of the lines establishing a correlation between the intensities of the lines specific to the element to be

Abstract: The invention relates to a method of fabricating an array of capillaries of a chip, the method comprising the steps consisting in depositing at least one layer of a meltable or polymerizable construction material on a support plate, focusing and moving a laser beam on and over said layer respectively to melt or polymerize the material so as to form the side walls of the capillaries, and then fastening a cover plate on the side walls of the capillaries. The invention also provides a chip including an array of capillaries in which chemical or biological molecules are fixed, and a chip including an array of chromatography and/or electrophoresis capillaries.

Abstract: The invention relates to a method of analysing molecular targets contained in a complex mixture, comprising the following steps consisting in: a) bringing the mixture of molecular targets to be analysed into contact with an array of different types of primary probes, whereby each type of primary probe forming the array can bind specifically to a type of target selected from among the molecular targets, under conditions that enable specific binding between the molecular targets and the primary probes; b) optionally eliminating the primary probes that are not bound specifically to a molecular target; c) separating the molecular targets and the primary probes which are bound specifically in a probe/target complex, such as to recover the array of primary probes representing a fingerprint of the molecular targets to be analysed; and d) quantitatively analysing the primary probes eluted in step c.

Abstract: The invention relates to an image analysis process for measuring the signal on biochips organized into one or several blocks each comprising a large number of spots each composed of at least one probe, the said process being characterized in that it comprises the following steps: a deposit is made with a constant pitch, the spots appearing in the form of a periodic and regular signal; this periodicity property is used to identify the position of the blocks and the spots, this periodicity being searched for on projections of the image in the x and y directions.