Abstract: The present invention provides an apparatus to prevent the entrance of aquatic species into the intake watercourse having a U-shaped cross section with a narrow bottom and a wide surface, in which oppositely facing electrodes are installed vertically along the side walls on both sides of the intake watercourse, and the distance between the upper ends of the electrodes is made greater than the distance between the lower ends of the electrodes, and a voltage is impressed between the both electrodes. Since the electric barrier intensity is smallest at the surface of water and becomes greater as it goes toward the bottom of water, the river fish moving around the bottom of water make a U-turn when they reach the strong electric barrier, or alternatively, after reaching the strong electric barrier, they move upward to where the electric barrier is weaker and then make a U-turn, thereby the entrance of aquatic species into the intake watercourse can be prevented.