Abstract: Methods for fractionating a sample of biomolecules of the disclosure comprise (a) introducing a sample into a separating column; (b) separating biomolecules in the sample by molecular weight into a plurality of fractions along the separating column; and (c) placing the separating column into successive engagement with a plurality of wells and advancing into each of the wells one or more of the corresponding plurality of fractions. Methods of the disclosure further comprise detecting and identifying biomolecules in the fractions using microparticles to bind to the fractions, and novel approaches to pool the fractions followed by assays—such as immunoassays—that allow high throughput and automation capabilities.

Abstract: The invention provides compositions and methods useful for identifying, verifying or authenticating any type of sample, whether the sample, is biological or non-biological.