Abstract: The present invention provides a microfluidic chip for culturing microorganism and a method of operating the same. The microfluidic chip includes a first input unit, a second input unit, a connection unit connected to the first and second input units, a plurality of control valves, and a ring-shaped storage structure connected to the connection unit and having first and second growth chambers that store a microbial solution. The first and second input units provide first and second solutions, respectively. The first solution is transferred into the first or second growth chamber through the connection unit to treat and discharge a portion of the microbial solution. The second solution is transferred into the first or second growth chamber to discharge the first solution. The control valves are actuated to mix the second solution and remainder of the microbial solution in the ring-shaped storage structure.