Abstract: Embodiments of the invention generally provide methods and compositions for producing polyvalent antigens. In one aspect, the invention provides a method for producing a cross-linked antigen. In another aspect, the invention provides a method of using cross-linked products as antigens to immunize animals and induce strong immune responses.

Abstract: Embodiments of the invention generally provide methods and compositions for producing recombinant transglutaminases. The purified recombinant transglutaminases of the invention are reactive to a broad range of compounds and exhibit broad substrate activity. In one embodiment, Streptoverticillium mobaraense (ATCC 29032), and Streptoverticillium cinnamoneum (ATCC 11874) recombinant transglutaminase fusion proteins purified from E. coli are provided to a better yield, higher purity, and activity than hitherto possible by recombinant DNA technology.

Abstract: Embodiments of the invention generally provide methods and compositions for producing polyvalent antigens. In one aspect, the invention provides a method for producing a cross-linked antigen. In another aspect, the invention provides a method of using cross-linked products as antigens to immunize animals and induce strong immune responses.

Abstract: Embodiments of the invention generally provide methods and compositions for producing disease-specific antigens. In one aspect, the invention provides a method of producing an antigen specific for Alzheimer's disease. In another aspect, the invention provides a method of producing a polyvalent antigen for two or more diseases. In yet another aspect, compositions of antigens are prepared and provided to immunize animals and induce strong immune responses.

Abstract: A method for producing transglutaminase-reactive compounds is provided. In one aspect, transglutaminase reactivity of a compound is enhanced. In another aspect, transglutaminase non-reactive compounds are modified to be reactive with transglutaminase.

Abstract: The invention provides a method of producing a cross-linked compound by a biological agent. In one aspect, cross-linking a compound requires a change of color in the cross-linking reaction mixture. In another aspect, attaching one or more amino acid residues to the compound is also required. In yet another aspect, the compound is obtained, and denaturing the compound in the presence of a denaturant and refolding the compound are performed before cross-linking the compound by a solution of the biological agent.

Abstract: Embodiments of the invention generally provide methods and compositions for producing recombinant transglutaminases. The purified recombinant transglutaminases of the invention are reactive to a broad range of compounds and exhibit broad substrate activity. In one embodiment, Streptoverticillium mobaraense (ATCC 29032), and Streptoverticillium cinnamoneum (ATCC 11874) recombinant transglutaminase fusion proteins purified from E. coli are provided to a better yield, higher purity, and activity than hitherto possible by recombinant DNA technology.