Abstract: The present invention includes a method for detecting and isolating sugarcane proteins that interact with the HC-Pro and P1 proteins of SrMV and other proteins involved in gene silencing, particularly in sugarcane. The method uses a two hybrid assay with an HC-Pro, P1, or other silencing-related protein-containing bait protein and a prey protein containing a polypeptide encoded by a DNA molecule in a cDNA library. The method also includes identification of false positives through reverse two-hybrid assays and using in vitro techniques such as farwestern blots or pull down assays where plant physiological conditions may be replicated. Finally, interactions may be confirmed in planta. Some novel proteins used in and discovered using the these methods are also identified. Methods of using viral and plant proteins to regulate silencing in plants such as sugarcane are also discussed.

Abstract: A method of extracting and purifying recombinant protein(s) from transgenic plant matter is disclosed. Fractioning of juice that has been extracted from the plant matter is obtained by using a multiple stage filtering process that uses multiple stages of decreasing porosity (preferably screening) followed by preferably membrane type filters, ion exchange, membrane adsorber, and chromatographic processes.

Abstract: A method of extracting and purifying recombinant protein(s) from transgenic sugarcane is disclosed. Fractioning of sugarcane juice that has been extracted from the cane stalks is obtained by using a multiple stage filtering process that uses multiple stages of decreasing porosity (preferably screening) followed by preferably membrane type filters, ion exchange, membrane adsorber, and chromatographic processes.

Abstract: A method of extracting and purifying recombinant protein(s) from transgenic sugarcane is disclosed. Fractioning of sugarcane juice that has been extracted from the cane stalks is obtained by using a multiple stage filtering process that uses multiple stages of decreasing porosity (preferably screening) followed by preferably membrane type filters, ion exchange, membrane adsorber, and chromatographic processes.

Abstract: A protein expression and recovery system in which transgenes are expressed in target plant varieties. Transformation is accomplished through bombardment of embryonic calli with plasmid-coated tungsten particles. Transformed calli are grown on tissue culture medium to produce transgenic plants which are then screened for presence of the transgene DNA or the polypeptide of interest. Transgenic plants are then cultivated. Cultivated plants may be harvested for extraction of the polypeptide.

Abstract: Transgenic plants that are modified to produce fruits that have altered levels of soluble solids compared to non-transgenic species of the same species are provided. The transgenic plants are modified by introduction of DNA constructs that encode invertase operatively linked to DNA encoding regulatory regions that direct transcription of the DNA encoding invertase and to DNA encoding sequences that direct proper processing of the invertase through the secretory pathways of the plant and targeting of the invertase to the vacuole.In particular, DNA constructs encoding tomato plant vacuolar invertase in operative linkage with a developmentally regulated promoter region are provided. Preferred regulatory and structural DNA is obtained from genomic DNA clones and cDNA clones encoding tomato fruit vacuolar invertases from the commercial tomato plant, Lycopersicon esculentum, and wild tomato plant, Lycopersicon pimpinellifolium.

Abstract: Transgenic plants that express properly processed ruminant or ruminant-like lysozymes and that are resistant to bacterial pathogens, including both gram-negative and gram-positive bacteria, are provided. A preferred embodiment provides transgenic tobacco plants that express a sufficient concentration of properly processed bovine lysozyme c2 to render the plants less susceptible to bacterial plant pathogens.Methods and compositions for treatment of plants, seeds and other plant tissues prior to or after exposure or infection with bacterial plant pathogens are also provided. In particular, compositions and methods of contacting plants with such compositions that contain a concentration of bovine lysozyme c2 or other ruminant or ruminant-like lysozyme are provided.A signal sequence that is effective for properly processing heterologous proteins that are expressed in transgenic plants is also provided.