Abstract: The present disclosure provides, at least, sterile fish and methods for producing sterile fish.

Abstract: Disclosed herein are genomically modified livestock animals and methods to provide them that express the SLICK phenotype. The animals disclosed herein express a truncated allele for the prolactin receptor (PRLR) gene. When expressed, the livestock animals produce a PRLR that is missing up to the terminal 148 amino acid (aa) residues of the protein all ranges and values within the explicitly stated range are contemplated: e.g., from 148 to 69. Animals expressing SLICK have superior thermoregulatory ability compared to non-slick animals and experience a less drastic depression in milk yield during the summer.

Abstract: Methods, uses, and animals for introgression of alleles between animals, including SNPs. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell with a mismatch in the binding of the targeting endonuclease and the targeted site.

Abstract: Disclosed herein are animals that are modified genetically to express one or more introduced sry alleles or to have a knockout of an existing sry allele. In various embodiments, the sry allele is inserted in at least one X chromosome of an XX resulting in an animal having two X chromosomes and appearing phenotypically male and sterile. In some embodiments the animal is modified to express multiple copies of sry inserted throughout its genome including the X chromosomes and the autosomes. In still other embodiments, the sry allele is inserted in multiple allosome sites. In these embodiments, XX individuals will be sterile and phenotypically male. XY individuals will be male and fertile but offspring will have an opportunity to inherit multiple sry alleles. In this embodiment, progeny of a male carrier will be sterile and phenotypically male if they are genotypically XX and express sry. Male progeny will be fertile and normal but will carry a hereditable copy of sry in their allosomes.

Abstract: Disclosed herein are genomically modified livestock animals and methods to provide them that express the SLICK phenotype. The animals disclosed herein express a truncated allele for the prolactin receptor (PRLR) gene. When expressed, the livestock animals produce a PRLR that is missing up to the terminal 148 amino acid (aa) residues of the protein all ranges and values within the explicitly stated range are contemplated: e.g., from 148 to 69. Animals expressing SLICK have superior thermoregulatory ability compared to non-slick animals and experience a less drastic depression in milk yield during the summer.

Abstract: Methods and kits to determine the presence of exogenous alleles within a native haplotype are provided. Introduction of foreign alleles into livestock genomes has provided the ability to introduce specific desirable traits. The present disclosure provides methods to identify the presence of exogenous alleles that foreign to a haplotype at a target locus, and identify specific markers that are native to the haplotype. Identification of exogenous genes at a target locus, flanked by native markers is indicative that the exogenous gene is present through molecular engineering. Conversely, the presence of an exogenous gene that are only partially flanked by native markers is indicative that the allele is present due to sexual breeding.