Abstract: The present invention relates to a method for providing information for colorectal cancer diagnosis, a composition for diagnosing colorectal cancer, and a kit comprising same, and, more particularly, to: a method for providing information for colorectal cancer diagnosis by using a primer for specifically amplifying plural methylated colorectal cancer marker genes; a composition for diagnosing colorectal cancer; and a kit comprising same.

Abstract: The present invention relates to a method of detecting methylation of an SDC2 gene, a composition for detecting methylation of an SDC2 gene, and a kit comprising same and, more Particularly, to: a method of detecting methylation of an SDC2 gene by using primers for specifically amplifying a methylated SDC2 gene and a probe capable of complementarily hybridizing with a methylated SDC2 gene that has been specifically amplified by the primers; a composition for detecting methylation of an SDC2 gene; and a kit comprising same.

Abstract: The present disclosure relates to a method of detecting methylation of target DNA in a multiplex manner and a composition for detecting methylation of target DNA, and more particularly to a method for detecting methylation of target DNA, comprising: constructing an oligonucleotide, which comprises a target-specific sequence capable of binding complementarily to the target DNA and a universal primer that does not bind complementarily to the target DNA; linearly amplifying the target DNA for linear target enrichment by using the oligonucleotide as a primer; and amplifying the linearly amplified target DNA using an oligonucleotide, which is capable of binding complementarily to the linearly amplified target DNA, a universal primer, and a probe.

Abstract: The present disclosure relates to a method of detecting methylation of target DNA in a multiplex manner and a composition for detecting methylation of target DNA, and more particularly to a method for detecting methylation of target DNA, comprising: constructing an oligonucleotide, which comprises a target-specific sequence capable of binding complementarily to the target DNA and a universal primer that does not bind complementarily to the target DNA; linearly amplifying the target DNA for linear target enrichment by using the oligonucleotide as a primer; and amplifying the linearly amplified target DNA using an oligonucleotide, which is capable of binding complementarily to the linearly amplified target DNA, a universal primer, and a probe.

Abstract: A method for detecting the methylation of promoters using HpaII, a methylation-sensitive restriction enzyme. In such method, DNAs, derived from clinical samples or subjects to be diagnosed, are cut with HpaII, the cut DNAs are amplified by PCR with primers capable of amplifying CpG islands, and the presence or absence of the PCR amplification products is determined using a DNA chip for methylation detection. Unlike prior approaches, the inventive method allows the methylation of gene promoters to be detected in a simple and economical manner, and thus is useful for the diagnosis of diseases such as cancer that are characterized by methylation of gene promoters.

Abstract: Methylated promoters of colon cancer-specific genes and use thereof. Various disclosed aspects of the invention include methylated promoters of the colon cancer specific expression-decreased genes, microarrays for cancer diagnosis on which the methylated promoters are immobilized, and cancer diagnosis kits containing the methylated promoters. The methylated promoters of colon cancer-specific expression-decreased genes have utility for early detection of cancer and as targets for screening new drugs useful in the early treatment of cancer.

Abstract: A method for detecting the methylation of promoters using HpaII, a methylation-sensitive restriction enzyme. In such method, DNAs, derived from clinical samples or subjects to be diagnosed, are cut with HpaII, the cut DNAs are amplified by PCR with primers capable of amplifying CpG islands, and the presence or absence of the PCR amplification products is determined using a DNA chip for methylation detection. Unlike prior approaches, the inventive method allows the methylation of gene promoters to be detected in a simple and economical manner, and thus is useful for the diagnosis of diseases such as cancer that are characterized by methylation of gene promoters.

Abstract: A method for the linear amplification of RNA using a high-heel primer, in which annealing and extension are performed at the same temperature using a high-heel primer. A DNA primer rather than an RNA primer is used, to provide a highly stable linear amplification process that can easily amplify even a small amount of sample, allowing a sufficient amount of target for microarray tests to be obtained from a nanogram level of total RNA by one linear amplification process using a high-heel primer. The inventive method shows a high correlation between RNAs of the same origin regardless of RNA amounts and purification methods, and efficiently achieves linear amplification within a short time at low cost as compared to prior methodology.