Abstract: An eco-friendly quarantine composition has a function of killing phospholipid enveloped viruses, The eco-friendly quarantine composition includes: 10 to 40 wt. % of polyoxyethylene lauryl ether, 0.01 to 10 wt. % of polyoxyethylene 2-ethylhexyl ether, 0.01 to 10 wt. % of polyoxyethylene polyoxypropylene alkyl ether, and a balance of solvent, based on the total 100 wt. % of the composition. The eco-friendly quarantine composition may have 100% of SARS-CoV-2 virus removal rate in 1 minute and an effect of killing 100% of highly pathogenic avian influenza virus in 1 to 10 minutes. The composition has low cytotoxicity such that IC50 value (%) for human epithelial keratinocyte cells (HaCaT), human bronchial epithelial cells (BEAS-2B), and human monocyte cells (THP-1) is in a range of 0.003 to 0.0045, and effects of having antibacterial activity against non-pathogenic strains, and pathogenic strains.

Abstract: The present invention relates to a prime editing-based gene editing composition with enhanced editing efficiency, a method for gene editing by using the composition, a gene editing kit, and a method for construction of a gene-modified mammal. The prime editor developed in the present invention exhibits remarkably enhanced genome editing efficiency and target specificity and mutant animal models constructed by using same were observed to perform mutation transmission to the next generation and a phenotype change in the next generation. Thus, the enhanced prime editor or a gene editing composition comprising same can find advantageous applications in various purposes, such as the construction and research of humanized animal models, the genetic engineering technical field, and therapeutic means for genetic diseases.