Abstract: The present disclosure provides a sample extraction device, which includes a plurality of chambers arranged on a pedestal. The chamber includes a plurality of first chambers and two mixing chambers. The pedestal is internally provided with a first passage for communicating the two mixing chambers, and a sample extraction chamber is arranged in the first passage. The pedestal is further internally provided with a second passage for transfer and export of a liquid, and connection or disconnection between the two mixing chambers and the second passage is controlled by a valve. Sample extraction processes of the above technical solutions are carried out in a fully enclosed device, avoiding contamination of samples and extraction reagents by external environments, and avoiding environmental contamination caused by aerosols generated during the sample extraction processes. The sample extraction device is particularly suitable for extraction of highly pathogenic samples.

Abstract: Provided are a cell line PA/PB1 HEK293T (Teton) for screening an influenza virus polymerase assembly inhibitors and a construction method thereof. The PA/PB1 HEK293T (Teton) cell line is constructed by integrating a vector expressing PA-GlucC fusion protein and a vector expressing GlucN-PB1 fusion protein into a HEK293T (Teton) cell via a triple plasmid system of pMDLg/pRRE, pRSV-Rev and pMD2.G. The HEK293T (Teton) cell line is formed by transforming a TOP10 competent cell with a synthesized Teton-3G gene fragment ligated to a lentiviral vector FG.

Abstract: Provided are a cell line PA/PB1 HEK293T (Teton) for screening an influenza virus polymerase assembly inhibitors and a construction method thereof. The PA/PB1 HEK293T (Teton) cell line is constructed by integrating a vector expressing PA-GlucC fusion protein and a vector expressing GlucN-PB1 fusion protein into a HEK293T (Teton) cell via a triple plasmid system of pMDLg/pRRE, pRSV-Rev and pMD2.G. The HEK293T (Teton) cell line is formed by transforming a TOP10 competent cell with a synthesized Teton-3G gene fragment ligated to a lentiviral vector FG.