Abstract: A system and method for voice control of operating room electrical equipment. The system comprises an electrosurgical generator a controller with a memory, a graphical user interface controlled by said controller, a power module, a field programmable gate array, and a voice recognition module connected to said field programmable gate array, a data storage connected to said controller in said electrosurgical generator; and electrical operating room equipment connected to said voice recognition module, wherein said electrical operating room equipment is configured to receive and decrypt encrypted commands from said voice recognition module. The electrical operating room equipment may said electrosurgical generator or a robotic surgical system or other electrical equipment in an operating room. The connection between said electrical operating room equipment and said voice recognition module may be wireless.

Abstract: A method for applying cold atmospheric plasma treatment to target tissue comprising the steps of selecting through a graphical user interface a particular soft tissue sarcoma cell line associated with target tissue, retrieving, with said computing device, settings data associated with said selected soft tissue sarcoma cell line from a database of cell line data and associated settings data in a storage, and applying, with said computing device, said retrieved settings data to a cold atmospheric plasma system.

Abstract: An integrated gas-enhanced electrosurgical generator. The generator comprises a high frequency power module, a low frequency power module and a gas module. The high frequency power module adapted to generate an electrical energy having a band of frequencies centered around a first frequency, wherein the electrical energy has a first power as the first frequency and a second power lower than the first power at a second frequency lower than the first frequency. The low frequency power module having an input connected to an output of the high frequency module. The low frequency module comprises a resonant transformer comprising a ferrite core, a primary coil and a secondary coil, the secondary coil having a larger number of turns than the primary coil, wherein the resonant transformer has a resonant frequency equal to the second frequency. The gas module is adapted to control a flow of an inert gas.

Abstract: An attachment for a gas-assisted electrosurgical device. The attachment has a housing having a channel within it, an electrosurgical blade and an electrical connector. The electrosurgical blade has a conductive member comprising an elongated distal portion and a proximal portion, the distal portion having a width greater than a width of the proximal portion, the width of the distal portion being at least three times the thickness of the distal portion, and a coating on at least a portion of said conductive member.

Abstract: A method for using Cold Atmospheric Plasma based T cell therapy for treating cancer including treating tumor cells with cold atmospheric plasma to permeabilize the tumor cells, condensing a mammalian CRISPR plasmid vector into compact nanostructures using one of poly-L-lysine (PLL) and Star-shaped poly(ethylene glycol)-block-polyethylenimine, and transfecting tumor cells with the catalytically inactive condensed CRISPR plasmid vector containing transcriptional activators (dcas9-VP64-sgRNA-CRISPRa) to elicit immune responses by enhancing the presentation of tumor-associated antigens.

Abstract: A method for performing cold atmospheric plasma treatment of respiratory infections or lung cancer having a source of a carrier gas, a cold atmospheric plasma generator connected to the source of carrier gas, a source of compressed air, a humidifier connected to the source of compressed air, a source of oxygen, a ventilator having inputs connected to an output of the humidifier and the source of oxygen, a mixer having an interior chamber formed from a dielectric, an active electrode inside the interior chamber, and an outer electrode connected to ground, wherein the mixer has a fluid input port connected to a gas output of the cold atmospheric plasma generator and an output of the ventilator, and a delivery member connected to an output of the mixer for delivering combined humidified air and cold atmospheric plasma to a respiratory system of a patient.

Abstract: A method for producing cold atmospheric plasma-produced exosomes ex vivo for use in systemic treatment of cancer. The method comprises isolating patient cancerous tumor primary cells, culturing isolated patient cancerous tumor primary cells in appropriate culture media, sequencing said isolated patient cancerous tumor primary cells and identifying mutant genes, transfecting mutant genes into pots of cells, wherein the transformed cells overexpress a unique mutant protein, treating the pots of transformed cells with cold atmospheric plasma to cause apoptosis of said transformed cells, wherein apoptotic cells secrete exosomes containing oxidized mutant proteins, isolating said exosomes containing oxidized mutant proteins, and storing said exosomes. The method may further comprise injecting said exosomes into a patient to systemically treat cancer.

Abstract: A method for producing microvesicles ex vivo for use in systemic treatment of cancer. The method comprises isolating patient cancerous tumor primary cells, culturing isolated patient cancerous tumor primary cells in appropriate culture media, treating cultured patient cancerous tumor primary cells non cold atmospheric plasma, after apoptosis of cultured patient cancerous tumor primary cells occurs, collecting apoptotic cell-derived extracellular microvesicles from the culture media by differential centrifugation, directly applying apoptotic cell-derived extracellular microvesicles to one of a naïve T cell culture or a dendritic cell culture, isolating antigen specific T cells from said one of a T cell culture and a dendritic cell culture, and storing said isolated antigen specific T cells.

Abstract: An electrosurgical system and method for control of an electrosurgical system with an enhanced reality display system wirelessly connected to the gas-enhanced electrosurgical system. The electrosurgical system comprises a gas-enhanced electrosurgical generator, a voice-recognition system connected to said gas-enhanced electrosurgical generator, a robotic system, and an enhanced reality display system wirelessly connected to said gas-enhanced electrosurgical system. The gas-enhanced electrosurgical generator may comprise a power module, a gas control module, and a control module, wherein said control module is configured to provide voice control of said power module and said gas module.

Abstract: A configurable electrosurgical generator front panel. The configurable electrosurgical generator front panel has a front panel frame, a display screen or tablet computing device, and a plurality of connectors for connecting accessories to the front panel frame. The front panel in the front panel assembly provides a novel structure for inserting a screen or tablet device into the front panel frame and securing it in place.

Abstract: A system and method for using cold atmospheric plasma to treat an air flow to inactivate viruses and sterilize the air flow. The system comprises a source of a carrier gas, a humidifier connected to said source of a carrier gas, a source of a feed gas, a plasma generator configured to plasmatize said carrier gas into a plasma; and a mixer having an interior chamber, an active electrode inside said interior chamber and connected to an electrical output of said plasma generator, an outer electrode connected to a ground and a dielectric barrier between said inner electrode and said outer electrode, wherein said mixer has a first fluid input port connected to said source of a carrier gas, a second fluid input connected to said source of a feed gas and a fluid output connected to a ventilation system.

Abstract: A system and method for voice control of operating room electrical equipment. The system comprises an electrosurgical generator having a controller with a memory, a graphical user interface controlled by said controller, a power module, a field programmable gate array, and a voice recognition module connected to said field programmable gate array, a data storage connected to said controller in said electrosurgical generator; and electrical operating room equipment connected to said voice recognition module, wherein said electrical operating room equipment is configured to receive and decrypt encrypted commands from said voice recognition module. The audio input to the voice recognition system may be a microphone chip in a electrosurgical accessory.

Abstract: A configurable electrosurgical generator front panel. The configurable electrosurgical generator front panel has a front panel frame, a display screen or tablet computing device, and a plurality of connectors for connecting accessories to the front panel frame. The front panel in the front panel assembly provides a novel structure for inserting a screen or tablet device into the front panel frame and securing it in place.

Abstract: A method for treating cancer by treating target cancer cells with cold atmospheric plasma to induce apoptosis of the target cancer cells through the degradation of Histone RNA during S-phase induced by oxidation of RNA.

Abstract: A cold plasma accessory having a hand piece having within in it flexible tubing, wiring, a PCB board connected to the wiring, and a conductive connector tube electrically connected to the PCB board, the conductive connector tube having an inner channel, a rigid tube extending from the distal end of the hand piece, and an electrode within the rigid tube. The electrode comprises a conductive connector having a contact surface contacting the conductive connector tube, a distal end surface facing away from the conductive connector tube, and a channel extending through a center of the conductive connector, the channel being fluidly connected to the flexible tubing, the distal end surface of the conductive connector being outside of the channel in the conductive connector, and a conductive wire connected to the distal end surface of the conductive connector.

Abstract: A method for bioengineering exosomes for cancer treatment. The method comprises the steps of isolating target tumor cells from a tissue sample, culturing the isolated target tumor cells, treating the cultured target tumor cells with cold atmospheric plasma to release exosomes from the target tumor cells, and collecting the released exosomes. The step of culturing the target tumor cells may comprise seeding the isolated target tumor cells in wells and incubating the target tumor cells in a culture media, for example, for 24 hours. The culture media may comprise Dulbecco's Modified Eagle Medium, an antibiotic and 10% fetal bovine serum. The step of culturing the target tumor cells may further comprise replacing the culture media with Dulbecco's Modified Eagle Medium and an antibiotic. The target tumor cells may comprise, for example, one of U87 cells, KKU-055 cells and triple negative MDA-MB-231 cells.

Abstract: A configurable electrosurgical generator front panel. The configurable electrosurgical generator front panel has a front panel frame, a display screen or tablet computing device, and a plurality of connectors for connecting accessories to the front panel frame. The front panel in the front panel assembly provides a novel structure for inserting a screen or tablet device into the front panel frame and securing it in place.

Abstract: A method for applying cold atmospheric plasma treatment to target tissue comprising the steps of treating a patient with a pharmaceutical for suppressing apolipoprotein E genes, selecting through a graphical user interface a particular soft tissue sarcoma cell line associated with target tissue, retrieving, with said computing device, settings data associated with said selected soft tissue sarcoma cell line from a database of cell line data and associated settings data in a storage, and applying, with said computing device, said retrieved settings data to a cold atmospheric plasma system.

Abstract: A system and method for voice control of operating room electrical equipment. The system comprises an electrosurgical generator a controller with a memory, a graphical user interface controlled by said controller, a power module, a field programmable gate array, and a voice recognition module connected to said field programmable gate array, a data storage connected to said controller in said electrosurgical generator; and electrical operating room equipment connected to said voice recognition module, wherein said electrical operating room equipment is configured to receive and decrypt encrypted commands from said voice recognition module. The electrical operating room equipment may said electrosurgical generator or a robotic surgical system or other electrical equipment in an operating room. The connection between said electrical operating room equipment and said voice recognition module may be wireless.

Abstract: A system and method for operating a touchscreen of a gas-enhanced electrosurgical generator. The generator has a display module and a primary controller. The display module has a plurality of touch sensors, a PCB power relay and a CPU. The method comprises selecting electrosurgery settings through a graphical user interface, activating through an input device plasma delivery from the gas-enhanced electrosurgical generator, disabling the plurality of touch sensors through software running on the primary controller, disconnecting power from the plurality of touch sensors with the PCB power relay in response to the disabling of the plurality of touch sensors, applying power to an electrode in the plasma accessory connected to the gas-enhanced electrosurgical generator, and de-activating through an input device plasma delivery from the gas-enhanced electrosurgical generator to the plasma accessory connected to the gas-enhanced electrosurgical generator.