Abstract: [Problem] To provide a glucose dehydrogenase with high thermal stability to be used in blood sugar measurement. [Solution to Problem to problem] A modified glucose dehydrogenase with an excellent property of high thermal stability that can be obtained by modifying some of amino acids constituting a protein such as a wild-type glucose dehydrogenase, a polynucleotide encoding the enzyme, a method for manufacturing the enzyme, a method for measuring glucose using the enzyme, a measuring reagent composition and a biosensor, and methods for manufacturing the measuring reagent composition and the biosensor.

Abstract: [Problem] To provide a glucose dehydrogenase with high thermal stability to be used in blood sugar measurement. [Solution to Problem to problem] A modified glucose dehydrogenase with an excellent property of high thermal stability that can be obtained by modifying some of amino acids constituting a protein such as a wild-type glucose dehydrogenase, a polynucleotide encoding the enzyme, a method for manufacturing the enzyme, a method for measuring glucose using the enzyme, a measuring reagent composition and a biosensor, and methods for manufacturing the measuring reagent composition and the biosensor.

Abstract: The present invention addresses the problem of providing: a glucose dehydrogenase; a polynucleotide encoding the enzyme; a method for producing the enzyme; a method for measuring glucose using the enzyme; a measurement reagent composition; and a biosensor. The present invention pertains to a protein that has any of amino acid sequences (a), (b) and (c) and has a glucose dehydrogenase activity, etc.: (a) an amino acid sequence represented by SEQ ID NO: 3, 6, 15 or 16; (b) an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 3, 6, 15 or 16 by deleting, substituting or adding 1-3 amino acids; and (c) an amino acid sequence having 90% or more identity to the amino acid sequence represented by SEQ ID NO: 3 or 15 or 85% or more identity to the amino acid sequence represented by SEQ ID NO: 6 or 16.

Abstract: The present invention relates to a protein having glucose dehydrogenase activity selected from: (a) an amino acid sequence represented by SEQ ID NO: 3; (b) an amino acid sequence in which 1 to 3 amino acids are deleted from, replaced in or added to the amino acid sequence represented by SEQ ID NO: 3; (c) an amino acid sequence which has at least 80% identity with the amino acid sequence represented by SEQ ID NO: 3 and whose N-terminus is SS; or (d) an amino acid sequence which has at least 80% identity with the amino acid sequence represented by SEQ ID NO: 3, and does not contain a sequence represented by SEQ ID NO: 8 at its N-terminus. The invention also includes a polynucleotide encoding the enzyme, a method for manufacturing the enzyme, a method for measuring glucose using the enzyme, a measuring reagent composition and a biosensor.

Abstract: The present invention relates to a protein having glucose dehydrogenase activity selected from:(a) an amino acid sequence represented by SEQ ID NO: 3; (b) an amino acid sequence in which 1 to 3 amino acids are deleted from, replaced in or added to the amino acid sequence represented by SEQ ID NO: 3;(c) an amino acid sequence which has at least 80% identity with the amino acid sequence represented by SEQ ID NO: 3 and whose N-terminus is SS; or(d) an amino acid sequence which has at least 80% identity with the amino acid sequence represented by SEQ ID NO: 3, and does not contain a sequence represented by SEQ ID NO: 8 at its N-terminus. The invention also includes a polynucleotide encoding the enzyme, a method for manufacturing the enzyme, a method for measuring glucose using the enzyme, a measuring reagent composition and a biosensor.

Abstract: A flavin-conjugated glucose dehydrogenase which is composed of proteins having the following amino acid sequence (a), (b) or (c), and having glucose dehydrogenase activity: (a) an amino acid sequence represented by SEQ ID NO: 2, 3, 5, 6, 8 or 9; (b) an amino acid sequence in which one or more amino acids are deleted from, replaced in or added to the amino acid sequence represented by SEQ ID NO: 2, 3, 5, 6, 8 or 9; (c) an amino acid sequence having at least 85% identity with the amino acid sequence represented by SEQ ID NO 2 or 3, at least 95% identity with the amino acid sequence represented by SEQ ID NO 5 or 6, or at least 80% identity with the amino acid sequence represented by SEQ ID NO 8 or 9.

Abstract: [Problems] To provide a glucose dehydrogenase, a polynucleotide encoding the enzyme, a method for manufacturing the enzyme, a method for measuring glucose using the enzyme, a measuring reagent composition, and a biosensor. [Solutions] A flavin-conjugated glucose dehydrogenase which is composed of proteins having the following amino acid sequence (a), (b) or (c), and having glucose dehydrogenase activity: (a) an amino acid sequence represented by SEQ ID NO: 2, 3, 5, 6, 8 or 9; (b) an amino acid sequence in which one or more amino acids are deleted from, replaced in or added to the amino acid sequence represented by SEQ ID NO: 2, 3, 5, 6, 8 or 9; (c) an amino acid sequence having at least 85% identity with the amino acid sequence represented by SEQ ID NO 2 or 3, at least 95% identity with the amino acid sequence represented by SEQ ID NO 5 or 6, or at least 80% identity with the amino acid sequence represented by SEQ ID NO 8 or 9.

Abstract: The purpose of the invention is to provide flavin-conjugated glucose dehydrogenase having little variation in activity in the typical biosensor measurement temperature range (10-40° C.) and a method for measuring glucose using same. The present invention relates to flavin-conjugated glucose dehydrogenase having the following properties (1)-(3) and the like: (1) action: shows glucose dehydrogenase activity in the presence of an electron acceptor; (2) substrate specificity: the activity value is 10% or less relative to maltose, D-galactose, D-fructose, sorbitol, lactose, and sucrose when the activity value relative to D-glucose is taken to be 100%; and (3) temperature characteristics: the range of the activity value at 10-40° C. is 20-150% when the activity value at 30° C. is taken to be 100%.