Abstract: Improved method for producing fats and oils rich in 1,3-disaturated-2-unsaturated-triglycerides (e.g. 1,3-distearyl-2-oleyl compound and 1-palmityl-2-oleyl-3-stearyl compound) useful as a cacao butter substitute, which comprises cultivating a microorganism being capable of assimilating a higher alkyl derivative and producing fats and oils containing 1,3-disaturated-2-unsaturated-triglycerides in a specific medium containing a mixed carbon source comprising at least one stearyl compound and at least one of other carbon compounds selected from a palmityl compound, an oleyl compound and a saccharide under an aerobic condition, collecting the cells of the microorganism and recovering the fats and oils from the cells.

Abstract: A method for producing a cacao butter substitute which comprises cultivating a microorganism capable of producing fats and oils rich in 1,3-disaturated-2-unsaturated-triglycerides belonging to the genus Endomyces, Rhodotorula, Lipomyces or Rhodosporidium, such as Endomyces vernalis, Rhodotorula gracilis, Rhodotorula glutinis, Rhodotorula graminis, Rhodosporidium toruloides, Lipomyces starkeyi and Lipomyecs lipofer, under an aerobic condition, collecting the cells, isolating fats and oils rich in 1,3-disaturated-2-unsaturated-triglycerides from the cells, and optionally subjecting the fats and oils to fractionation.