Abstract: A cell culture system includes a culture unit having a cutout portion on which a bag housing component having a pressing member that presses the culture bag is mounted. A bag placed on the bag housing component, a medium vessel supplying a medium to the bag, a waste liquid vessel into which the medium is removed from the bag, a pumping means provided in a member connecting the bag and the medium vessel, and a pumping means provided in a member connecting the bag and the waste liquid vessel are provided on the unit, wherein a distance from a bottom surface of the unit to a culture surface is shorter than 20 mm, a liquid thickness of the medium becomes uniform when the pressing member presses the bag.

Abstract: In the production of a culture vessel in which a low cell-adhesive polymer as a coating agent is applied to a culture surface having a plurality of concave parts formed thereon, a use amount of the coating agent can be reduced. The low cell-adhesive polymer as the coating agent is applied to a surface of a culture base material in advance, and subsequently the culture base material is subjected to processing to form the culture vessel having the surface of the culture base material coated with the coating agent as the culture surface.

Abstract: An object of an aspect of the present invention is to provide a new organic-inorganic hybrid membrane composite excellent in permeance and a relevant technique thereof. An organic-inorganic hybrid membrane composite in an aspect of the present invention includes a porous support layer and an organic-inorganic hybrid membrane formed on the porous support layer, an air permeation amount is 10000 L/(m2·h) or less, the organic-inorganic hybrid membrane contains a matrix polymer and a gas-selective inorganic filler, a contained amount of the gas-selective inorganic filler relative to the organic-inorganic hybrid membrane is 35% by mass or more, the gas-selective inorganic filler contains zeolite, a framework density in a case where all T elements of the zeolite are silicon is 16.0 T/1000 ?3 or less, and a membrane thickness of the organic-inorganic hybrid membrane is 0.05 ?m or more and 50 ?m or less.

Abstract: Provided is a culture container base material made of a polyolefin material, capable of readily forming a culture container for culturing adherent cells. The culture container base material is for culturing the adherent cells and made of the polyolefin material. At least a part of a surface of the base material is subjected to a surface treatment, where the surface of the base material is a culture surface of the culture container, and the surface subjected to the surface treatment has a static water contact angle of greater than 80° and a receding contact angle of less than 53°.

Abstract: A method for producing the cell culture vessel having the culture surface of cells includes: using a primary mold including a projecting portion to form an uneven pattern structure on a surface of a vessel base material and a secondary mold including a projecting portion to flatten a part of the surface of the vessel base material; pressing the primary mold against the vessel base material to form the uneven pattern structure on the vessel base material; pressing the secondary mold against a thin portion formed on the vessel base material to modify the thin portion to a flattened portion; and configuring the surface of the vessel base material including the uneven pattern structure formed with at least a part of the modified flattened portion as the culture surface to form the cell culture vessel.

Abstract: A culture vessel includes at least a first port used for medium supply and a second port used for medium discharge, and A medium supplying vessel serves as a mixing vessel for adjusting a concentration of A new medium to be supplied to the culture vessel. A cell culture method includes: transferring a portion of a used medium in the culture vessel from the culture vessel to the mixing vessel; creating a concentration-adjusted medium by mixing the new medium and the portion of the used medium in the mixing vessel; and filling the culture vessel with the concentration-adjusted medium by discharging a remaining used medium from the culture vessel while the concentration-adjusted medium is transferred from the mixing vessel to the culture vessel.

Abstract: Provided is a cell culture system capable of detecting a proliferative property of cells in a culture vessel at a desired timing during a culture when the cells are statically cultured in the culture vessel formed of a gas permeable member. A cell culture system is a system for statically culturing cells with a culture vessel at least partially formed of a gas permeable member. The cell culture system includes an oxygen concentration sensor that measures an oxygen concentration in a vicinity of a culture surface inside the culture vessel, and a proliferation detection unit that detects a proliferative property of the cells based on an oxygen permeability of the gas permeable member, a measurement value of the oxygen concentration sensor, an oxygen concentration around the culture vessel, and an oxygen consumption per cell of the cells.

Abstract: Provided is a cell culture vessel for culturing cells with a high density having excellent gas permeability and strength. A bag-shaped cell culture vessel of a closed system includes at least one port and mutually opposed planar substrates. At least one of the planar substrates is formed of a gas permeable film, the at least one gas permeable film has an outer surface provided with a protruding portion having a shape different from an inner surface shape of the gas permeable film, a culture space for culturing cells is provided in the inner surface side of the gas permeable film, and when the gas permeable film is brought in contact with a planar surface, a space enabling ventilation is formed between the gas permeable film and the planar surface by the protruding portion.

Abstract: Provided is a culture vessel for adherent cells that has a large culture portion surface area and ensures easily dissociating and recovering cells without consuming a medium or a dissociation solution more than necessary. The bag-shaped adherent cell culture vessel made of flexible packaging material includes a vessel body portion having a first vessel wall and a second vessel wall and one or more injecting/ejecting ports. The vessel body portion has an intermediate culture wall between the first vessel wall and the second vessel wall. A culture chamber is disposed in each of between the first vessel wall and the intermediate culture wall and between the second vessel wall and the intermediate culture wall, and a flow passage communicating between the respective culture chambers is disposed.

Abstract: An adherent cell culture substrate, which is a sheet-shaped substrate used for producing a culture vessel for adherent cells, includes a groove including a crest-shaped portion and a valley-shaped portion on one surface side of the substrate, in which a distance from a top end portion of the crest-shaped portion to the other surface of the substrate is 1 mm or less. Further, it is preferable that a cross section of the groove perpendicular to a direction in which the groove extends is formed in a substantially V-shape and an inclination angle of a side surface of the substantially V-shape of the groove is 80 degrees or less. Further, it is preferable that a plurality of the grooves are linearly arranged in parallel and the top end portions of the crest-shaped portions are formed in a linear shape.

Abstract: In the production of a culture vessel in which a low cell-adhesive polymer as a coating agent is applied to a culture surface having a plurality of concave parts formed thereon, a use amount of the coating agent can be reduced. The low cell-adhesive polymer as the coating agent is applied to a surface of a culture base material in advance, and subsequently the culture base material is subjected to processing to form the culture vessel having the surface of the culture base material coated with the coating agent as the culture surface.

Abstract: Provided is a culture container base material made of a polyolefin material, capable of readily forming a culture container for culturing adherent cells. The culture container base material is for culturing the adherent cells and made of the polyolefin material. At least a part of a surface of the base material is subjected to a surface treatment, where the surface of the base material is a culture surface of the culture container, and the surface subjected to the surface treatment has a static water contact angle of greater than 80° and a receding contact angle of less than 53°.