Patents by Inventor Takahide Kishimoto
Takahide Kishimoto has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11767349Abstract: The invention provides streptolysin O containing a polypeptide comprising an amino acid sequence having deletion of a polypeptide extending from a lysine residue at position 2 of SEQ ID NO: 1 to any of an alanine residue at position 31, a glutamate residue at position 32, a serine residue at position 33, and an asparagine residue at position 34, and also having deletion of any of an isoleucine residue at position 465 to an alanine residue at position 472 of SEQ ID NO: 1 and all of the subsequent amino acid residues, as well as variants thereof and related DNA, vectors, transformants, and methods of use thereof.Type: GrantFiled: May 26, 2017Date of Patent: September 26, 2023Assignee: TOYOBO CO., LTD.Inventors: Yosuke Sumida, Hiroaki Kitazawa, Takahide Kishimoto
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Patent number: 11591631Abstract: A glucose monitoring method and a glucose sensor, both of which use glucose dehydrogenase having a Michaelis constant (Km) for xylose of 600 mM or more and 3000 mM or less, and a Km for glucose of 0.1 mM or more and 100 mM or less, which provide for evaluating FADGDH in an aqueous system while reducing the practical influence of FADGDH on D-xylose.Type: GrantFiled: June 22, 2018Date of Patent: February 28, 2023Assignee: TOYOBO CO., LTD.Inventors: Yasuhiro Yamazaki, Atsushi Kawai, Takahide Kishimoto
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Patent number: 10913971Abstract: Provided is GDH with increased applicability to glucose sensors. A composition contains FADGDH, wherein when 0.1 mL of the composition is added to 2.9 mL of a solution containing 10 mM of trehalose and 1 mmol/L of potassium ferricyanide to give a glucose dehydrogenase activity of 500 U/mL and incubated at 37° C., the decrease in absorbance at 405 nm resulting from reduction of the potassium ferricyanide is less than 20 mAbs per minute.Type: GrantFiled: April 7, 2016Date of Patent: February 9, 2021Assignee: TOYOBO CO., LTD.Inventors: Yuu Utashima, Takahide Kishimoto
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Publication number: 20200325186Abstract: The invention provides streptolysin O containing a polypeptide comprising an amino acid sequence having deletion of a polypeptide extending from a lysine residue at position 2 of SEQ ID NO: 1 to any of an alanine residue at position 31, a glutamate residue at position 32, a serine residue at position 33, and an asparagine residue at position 34, and also having deletion of any of an isoleucine residue at position 465 to an alanine residue at position 472 of SEQ ID NO: 1 and all of the subsequent amino acid residues, as well as variants thereof and related DNA, vectors, transformants, and methods of use thereof.Type: ApplicationFiled: May 26, 2017Publication date: October 15, 2020Applicant: TOYOBO CO., LTD.Inventors: Yosuke SUMIDA, Hiroaki KITAZAWA, Takahide KISHIMOTO
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Publication number: 20200172950Abstract: A glucose monitoring method and a glucose sensor, both of which use glucose dehydrogenase having a Michaelis constant (Km) for xylose of 600 mM or more and 3000 mM or less, and a Km for glucose of 0.1 mM or more and 100 mM or less, which provide for evaluating FADGDH in an aqueous system while reducing the practical influence of FADGDH on D-xylose.Type: ApplicationFiled: June 22, 2018Publication date: June 4, 2020Applicant: TOYOBO CO., LTD.Inventors: Yasuhiro YAMAZAKI, Atsushi KAWAI, Takahide KISHIMOTO
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Patent number: 9994827Abstract: Object An object of the present invention is to provide a highly stable FVHO preparation and a low-hygroscopicity dried FVHO preparation. Means for achieving the object A method for producing a FVHO preparation comprising a step of allowing at least one member selected from phosphoric acid, casein peptone, D-glucosamine hydrochloride, melibiose, sorbose, lactose, fructose, melezitose, glucono-1,5-lactone, and ribitol; and a method for producing a dried FVHO preparation, comprising a step of allowing Bicine to coexist.Type: GrantFiled: January 10, 2013Date of Patent: June 12, 2018Assignee: TOYOBO CO., LTD.Inventors: Rie Hirao, Takahide Kishimoto, Shusaku Yanagidani
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Publication number: 20180066302Abstract: Provided is GDH with increased applicability to glucose sensors. A composition contains FADGDH, wherein when 0.1 mL of the composition is added to 2.9 mL of a solution containing 10 mM of trehalose and 1 mmol/L of potassium ferricyanide to give a glucose dehydrogenase activity of 500 U/mL and incubated at 37° C., the decrease in absorbance at 405 nm resulting from reduction of the potassium ferricyanide is less than 20 mAbs per minute.Type: ApplicationFiled: April 7, 2016Publication date: March 8, 2018Applicant: TOYOBO CO., LTD.Inventors: Yuu UTASHIMA, Takahide KISHIMOTO
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Patent number: 9796963Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity and that is suitable for use in SMBG. The present invention provides a flavin-bound glucose dehydrogenase having the following characteristics (1) to (5): (1) Temperature stability: stable at a temperature of 45° C. or lower; (2) stable at a pH range of 4.5 to 7.5; (3) substrate specificity: the reactivity to D-xylose, maltose, or D-galactose is 2% or less, based on the reactivity to D-glucose taken as 100%; (4) optimal activity temperature: 34 to 47° C.; and (5) optimal activity pH: 6.3 to 6.7.Type: GrantFiled: September 6, 2013Date of Patent: October 24, 2017Assignee: Toyobo Co., Ltd.Inventors: Yasuhiro Yamazaki, Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
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Patent number: 9657325Abstract: Provided is a flavin adenine dinucleotide-dependent glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 78% or more identity to the amino acid sequence of SEQ ID NO: 3, and having glucose dehydrogenase activity.Type: GrantFiled: April 21, 2016Date of Patent: May 23, 2017Assignee: Toyobo Co., Ltd.Inventors: Hiroshi Aiba, Yosuke Sumida, Yasuhiro Yamazaki, Yuu Utashima, Takahide Kishimoto
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Patent number: 9506043Abstract: A novel diaphorase; a method for producing the diaphorase; and use of the diaphorase are provided. The diaphorase comprises any one of the following polypeptides (a) to (c): (a) a polypeptide having the amino acid sequence of SEQ ID NO: 1, (b) a polypeptide having the amino acid sequence of SEQ ID NO: 1 in which one or several amino acid residues are substituted, deleted, inserted, added, and/or inverted, and having diaphorase activity, and (c) a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having diaphorase activity.Type: GrantFiled: December 19, 2012Date of Patent: November 29, 2016Assignee: TOYOBO CO., LTD.Inventors: Sachio Iwai, Takahide Kishimoto, Seiji Takeshima, Shusaku Yanagidani
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Patent number: 9506042Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity, specific activity, thermal stability, and the like, and that is suitable for use in SMBG sensors. The present invention provides a purified polypeptide comprising an amino acid sequence having at least 80% identity to the sequence of SEQ ID NO: 1 and having glucose dehydrogenase activity.Type: GrantFiled: December 29, 2014Date of Patent: November 29, 2016Assignee: Toyobo Co., Ltd.Inventors: Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroaki Kitazawa, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
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Patent number: 9487758Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.Type: GrantFiled: February 10, 2014Date of Patent: November 8, 2016Assignee: Toyobo Co., Ltd.Inventors: Yosuke Sumida, Hiroshi Aiba, Hiroshi Kawaminami, Rie Hirao, Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani
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Publication number: 20160265021Abstract: Provided is a flavin adenine dinucleotide-dependent glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 78% or more identity to the amino acid sequence of SEQ ID NO: 3, and having glucose dehydrogenase activity.Type: ApplicationFiled: April 21, 2016Publication date: September 15, 2016Applicant: TOYOBO CO., LTD.Inventors: Hiroshi AIBA, Yosuke SUMIDA, Yasuhiro YAMAZAKI, Yuu UTASHIMA, Takahide KISHIMOTO
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Patent number: 9404144Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. The flavin-binding glucose dehydrogenase of the invention has the following characteristics (1) and (4): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 68 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 15 mM or less; (3) Temperature stability: stable at a temperature of 55° C. or less; and (4) pH stability: stable at a pH range of 3.0 to 8.5.Type: GrantFiled: February 7, 2013Date of Patent: August 2, 2016Assignee: TOYOBO CO., LTD.Inventors: Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroshi Kawaminami, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
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Patent number: 9260699Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided.Type: GrantFiled: April 30, 2014Date of Patent: February 16, 2016Assignee: Toyobo Co., Ltd.Inventors: Yosuke Sumida, Hiroshi Aiba, Hiroshi Kawaminami, Rie Hirao, Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani
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Publication number: 20150344852Abstract: [Object] To provide: a novel diaphorase; a method for producing the diaphorase; and use of the diaphorase. [Solution] A diaphorase comprising any one of the following polypeptides (a) to (c): (a) a polypeptide having the amino acid sequence of SEQ ID NO: 1, (b) a polypeptide having the amino acid sequence of SEQ ID NO: 1 in which one or several amino acid residues are substituted, deleted, inserted, added, and/or inverted, and having diaphorase activity, and (c) a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having diaphorase activity.Type: ApplicationFiled: December 19, 2012Publication date: December 3, 2015Applicants: TOYOBO CO., LTD., TOYOBO CO., LTD.Inventors: Sachio IWAI, Takahide KISHIMOTO, Seiji TAKESHIMA, Shusaku YANAGIDANI
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Patent number: 9133446Abstract: The present invention provides AP that has a high specific activity and preferably has superior reactivity with respect to various luminescent substrates generally used for high-sensitivity immunoassay analysis. Further preferably, the present invention provides AP having a thermal stability higher than that of CIAP. The alkaline phosphatase of the present invention is derived from the genus Shewanella and has the following characteristics: (A) molecular weight: about 104,000; (B) optimum reaction pH: about 9.5; (C) stable pH range: 5.5 to 10.4; (D) thermal stability: 65° C., and (E) specific activity: 5,000 U/mg or more.Type: GrantFiled: August 22, 2013Date of Patent: September 15, 2015Assignee: TOYOBO CO., LTD.Inventors: Hiroshi Aiba, Takahide Kishimoto, Yoshiaki Nishiya
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Publication number: 20150240216Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity and that is suitable for use in SMBG. The present invention provides a flavin-bound glucose dehydrogenase having the following characteristics (1) to (5): (1) Temperature stability: stable at a temperature of 45° C. or lower; (2) stable at a pH range of 4.5 to 7.5; (3) substrate specificity: the reactivity to D-xylose, maltose, or D-galactose is 2% or less, based on the reactivity to D-glucose taken as 100%; (4) optimal activity temperature: 34 to 47° C.; and (5) optimal activity pH: 6.3 to 6.7.Type: ApplicationFiled: September 6, 2013Publication date: August 27, 2015Inventors: Yasuhiro Yamazaki, Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
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Patent number: 9040257Abstract: The present invention provides a host for genetic recombination useful in the production of heterologous proteins in a large scale, by suppressing the production of extracellular polysaccharides in the basidiomycetous yeasts. Cryptococcus sp. S-2 D11 strain (FERM BP-11482) which is characterized in that the production of extracellular polysaccharides is suppressed as compared with the parent strain.Type: GrantFiled: April 23, 2013Date of Patent: May 26, 2015Assignees: TOYOBO CO., LTD., NATIONAL RESEARCH INSTITUTE OF BREWINGInventors: Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani, Kazuo Masaki
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Publication number: 20150111280Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity, specific activity, thermal stability, and the like, and that is suitable for use in SMBG sensors. The present invention provides a purified polypeptide comprising an amino acid sequence having at least 80% identity to the sequence of SEQ ID NO: 1 and having glucose dehydrogenase activity.Type: ApplicationFiled: December 29, 2014Publication date: April 23, 2015Inventors: Yosuke SUMIDA, Rie HIRAO, Yuu UTASHIMA, Hiroaki KITAZAWA, Hiroshi AIBA, Takahide KISHIMOTO, Shusaku YANAGIDANI