Patents by Inventor Takahide Yokoi

Takahide Yokoi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210247358
    Abstract: An electrophoresis method, system, and gel recover biological substances from the gel with high efficiency. The method uses an electrophoresis gel having an injection hole into which biological substances are injected and a recovery hole from which the biological substances are recovered. The electrophoresis method includes injecting the biological substances into the injection hole, and applying an electric field penetrating the injection and recovery holes. A vertical axis in a downward direction as a positive direction is set as an X-axis, an axis which is perpendicular to the X-axis is set as a Y-axis, and coordinates of a bottom of the recovery hole are set as (XC, YC). The X coordinate XC of the bottom of the recovery hole satisfies XC>X1 when the biological substance is electrophoresed to coordinates (X1, YC) in the recovery hole from a bottom of the injection hole in the applying the electric field.
    Type: Application
    Filed: May 28, 2019
    Publication date: August 12, 2021
    Applicant: HITACHI HIGH-TECH CORPORATION
    Inventors: Mima OGAWA, Takahide YOKOI, Chihiro UEMATSU
  • Publication number: 20210189467
    Abstract: The present disclosure proposes a probe including a sequence recognition portion that is capable of specifically binding to a desired DNA sequence, and a first probe portion that includes a plurality of 3-dimensional structures arranged downstream from an end of the sequence recognition portion, in which the plurality of 3-dimensional structures are arranged in an order so that the order corresponds one-to-one with the DNA sequence.
    Type: Application
    Filed: December 2, 2020
    Publication date: June 24, 2021
    Inventors: Itaru YANAGI, Takahide YOKOI
  • Publication number: 20210132034
    Abstract: Conventionally, only a pair of electrodes is provided and nanopores arranged in parallel are connected by an electrolyte solution, and therefore a change in anion current to be measured is a sum of changes in ion currents generated in the respective nanopores.
    Type: Application
    Filed: December 16, 2020
    Publication date: May 6, 2021
    Inventors: Takahide Yokoi, Takashi Anazawa
  • Patent number: 10996211
    Abstract: An object of the present invention is to solve inhibition of biopolymer measurement in a nanopore, which involves three-dimensional conformation of a biopolymer containing a nucleic acid. The present invention provides a device for analyzing a biopolymer containing a nucleic acid, the device including: an array device including a plurality of thin film parts having a nanopore; a single common container and a plurality of individual containers capable of storing a measurement solution which is brought into contact with the thin films; and individual electrodes respectively provided in the plurality of individual containers, wherein: the measurement solution is introduced into each of the individual containers and the common container so as to be brought into contact with the thin films; and the measurement solution has a pH equal to or greater than pKa of a guanine base and contains cesium ions as electrolyte cations.
    Type: Grant
    Filed: October 20, 2016
    Date of Patent: May 4, 2021
    Assignee: HITACHI HIGH-TECH CORPORATION
    Inventors: Yusuke Goto, Takahide Yokoi
  • Publication number: 20210108168
    Abstract: Provided is a technique for easily trapping microbiota in a sample. A microbiota trapping device of the present disclosure includes a first solution tank having a first filter adapted to trap residue in a sample, and a second solution tank having a second filter adapted to trap bacteria in the sample. The second solution tank has a vent above the second filter.
    Type: Application
    Filed: September 8, 2020
    Publication date: April 15, 2021
    Inventor: Takahide YOKOI
  • Patent number: 10908143
    Abstract: Conventionally, only a pair of electrodes is provided and nanopores arranged in parallel are connected by an electrolyte solution, and therefore a change in an ion current to be measured is a sum of changes in ion currents generated in the respective nanopores.
    Type: Grant
    Filed: November 27, 2013
    Date of Patent: February 2, 2021
    Assignee: Hitachi, Ltd.
    Inventors: Takahide Yokoi, Takashi Anazawa
  • Publication number: 20200102587
    Abstract: Provided are a method and apparatus for forming clusters of amplified nucleic acid fragments without amplification bias, in a regular arrangement on a substrate. In the method according to the present invention, droplets enclosing the template nucleic acid are formed on a substrate that has a plurality of first surfaces having hydrophilicity and a second surface surrounding each of the plurality of first surfaces and being less hydrophilic than the first surfaces. Then, after a nucleic acid amplification reaction is performed in the droplets on the substrate, the droplets are removed and a nucleic acid amplification reaction is further performed on the substrate.
    Type: Application
    Filed: April 5, 2017
    Publication date: April 2, 2020
    Inventors: Takahide YOKOI, Chihiro UEMATSU, Naoshi ITABASHI
  • Publication number: 20190353636
    Abstract: Provided are a first tank; a second tank; a thin film having a nanopore, which communicates the first tank to the second tank, and disposed between the first and second tanks; a first electrode provided in the first tank; and a second electrode provided in the second tank. A wall surface of the nanopore has an ion adsorption preventing structure to prevent desorption/adsorption of an ion contained in a solution filling the first tank and/or the second tank, and a voltage is applied between the first and second electrodes to measure an ion current flowing through the nanopore.
    Type: Application
    Filed: January 10, 2017
    Publication date: November 21, 2019
    Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
    Inventors: Kazuma MATSUI, Yusuke GOTO, Rena AKAHORI, Takahide YOKOI, Michiru FUJIOKA
  • Publication number: 20190352699
    Abstract: The present invention is directed to provide new PCR measuring method and device. As one embodiment of the present invention, a DNA detection method for detecting DNA in a droplet being present in oil, the droplet containing the DNA and a fluorescent labeled probe, the fluorescent labeled probe being hybridized to the DNA, the method including: a first step of amplifying the DNA in the the droplet by a nucleic acid amplification reaction; and a second step of measuring a melting temperature of the fluorescent labeled probe and the DNA in the droplet is provided.
    Type: Application
    Filed: November 16, 2017
    Publication date: November 21, 2019
    Inventors: Junko TANAKA, Takahide YOKOI, Masao KAMAHORI, Yoshinobu KOHARA
  • Patent number: 10451584
    Abstract: Provided is a biomolecule measuring device capable of effectively reducing measurement noise occurring when measuring a biomolecule sample using a semiconductor sensor. This biomolecule measuring device generates a trigger to react a sample with a reagent after starting to send the reagent onto the semiconductor sensor that detects ion concentration (see FIG. 7).
    Type: Grant
    Filed: November 18, 2013
    Date of Patent: October 22, 2019
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Yoshimitsu Yanagawa, Takahide Yokoi, Naoshi Itabashi, Takayuki Kawahara, Sonoko Migitaka, Makiko Yoshida, Takamichi Muramatsu
  • Publication number: 20190292589
    Abstract: The purpose of the invention is to provide a method for analyzing biomolecules with which it is possible to easily suppress the occlusion of nanopores. The first embodiment of the invention is a method for analyzing biomolecules including a step for preparing a substrate having nanopores, a step for placing a sample solution including biomolecules and at least one compound selected from the group consisting of primary amines, secondary amines, guanidine compounds, and salts thereof on the substrate, and a step for detecting the changes in light or electrical signal generated when the biomolecules pass through the nanopores.
    Type: Application
    Filed: June 10, 2016
    Publication date: September 26, 2019
    Inventors: Michiru FUJIOKA, Yusuke GOTO, Takahide YOKOI
  • Patent number: 10274459
    Abstract: An aspect of the present invention includes performing electrophoresis of a nucleic acid sample to be analyzed labeled for each base type, generating waveform data of detected intensity by detecting a label signal for the each base type, selecting another peak position for each peak position of the waveform data for each base type, calculating relative signal intensity of signal intensity in each position relative to the signal intensity in the other selected position, and analyzing existence of each base type in a base sequence coordinate position of the nucleic acid sample by comparing the relative signal intensity of the nucleic acid sample to be analyzed and the relative signal intensity of a known nucleic acid sample in each peak position. Accordingly, acquiring information about a gene mutation in trace amounts existing in a target gene region highly sensitively with high precision is realized.
    Type: Grant
    Filed: July 31, 2013
    Date of Patent: April 30, 2019
    Assignee: Hitachi, Ltd.
    Inventors: Makiko Yoshida, Takahide Yokoi, Takashi Anazawa
  • Publication number: 20190120792
    Abstract: The invention provides a technique for retaining hydrophilicity of a surface of a substrate over a long period of time with a simple maintenance without changing the structure and characteristics of the substrate. A component includes a hydrophilic component having a hydrophilic surface, a protective layer that is formed on the surface and contains a soluble sub stance.
    Type: Application
    Filed: September 5, 2018
    Publication date: April 25, 2019
    Inventors: Takahide YOKOI, Yusuke GOTO, Kazuma MATSUI
  • Publication number: 20190086362
    Abstract: A traditional nanopore DNA sequencing method has a problem in that a signal analysis error may occur when a signal variation reflecting fluctuation in a base current is contained in a signal variation in a signal analysis. An electrolyte solution for biomolecule assays, containing D2O as a solvent, and/or containing an electrolyte that has Cs and Na, Na alone, Na and Li, or Li alone as a cation species in the electrolyte solution, or trishydroxyaminomethane, or a combination thereof is used in formation of a nanopore or in measurement.
    Type: Application
    Filed: March 7, 2017
    Publication date: March 21, 2019
    Applicant: Hitachi, Ltd.
    Inventors: Rena AKAHORI, Yusuke GOTO, Kazuma MATSUI, Takahide YOKOI
  • Publication number: 20190032126
    Abstract: A base sequence determination apparatus includes (1) a mobility correction unit that outputs a mobility correction signal obtained by mobility correction of a time-series signal of a wavelength spectrum corresponding to each base, (2) a deconvolution unit that executes processes for calculating a deconvoluted signal of the mobility correction signal respectively for a plurality of parameter candidates of point spread function, calculating variance of peak intervals for the calculated deconvoluted signal, specifying a parameter of the point spread function using the calculated variance, and outputting the deconvoluted signal corresponding to the point spread function having the specified parameter as an updated deconvoluted signal, (3) a peak extracting unit that extracts a peak waveform from the updated deconvoluted signal and outputs an updated peak-extracted signal, and (4) a sequence specifying unit that inputs the updated peak-extracted signal and determines a base sequence.
    Type: Application
    Filed: January 28, 2016
    Publication date: January 31, 2019
    Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
    Inventors: Yohei KAWAGUCHI, Tomohiro YASUDA, George CHALKIDIS, Takahide YOKOI
  • Publication number: 20180335417
    Abstract: An object of the present invention is to solve inhibition of biopolymer measurement in a nanopore, which involves three-dimensional conformation of a biopolymer containing a nucleic acid. The present invention provides a device for analyzing a biopolymer containing a nucleic acid, the device including: an array device including a plurality of thin film parts having a nanopore; a single common container and a plurality of individual containers capable of storing a measurement solution which is brought into contact with the thin films; and individual electrodes respectively provided in the plurality of individual containers, wherein: the measurement solution is introduced into each of the individual containers and the common container so as to be brought into contact with the thin films; and the measurement solution has a pH equal to or greater than pKa of a guanine base and contains cesium ions as electrolyte cations.
    Type: Application
    Filed: October 20, 2016
    Publication date: November 22, 2018
    Inventors: Yusuke GOTO, Takahide YOKOI
  • Patent number: 10030266
    Abstract: In the field of the next generation DNA sequencer, a method for integrating very high sensitive FET sensors having side gates and nanopores as devices used for identifying four kinds of base and for mapping the base sequence of DNA without using reagents, and a semiconductor device having selection transistors and amplifier transistors respectively corresponding to the FET sensors having side gates and nanopores respectively so as to be able to read the variation of a detection current based on the differences among the charges of the four kinds of base without deteriorating the detection sensitivity of the FET sensor, are presented.
    Type: Grant
    Filed: June 27, 2013
    Date of Patent: July 24, 2018
    Assignee: HITACHI, LTD.
    Inventors: Itaru Yanagi, Riichiro Takemura, Yoshimitsu Yanagawa, Takahide Yokoi, Takashi Anazawa
  • Publication number: 20180195105
    Abstract: The present invention optically obtains characteristic values of a plurality of biological samples from a specimen; compares the characteristic values with examination information associated with the same specimen obtained in advance; and calculates an indicator to determine, based on a result of the comparison, whether a process is continued to genetic analysis of the biological samples or a re-preparation of samples from the same specimen is performed. By this method, it is possible to achieve more reliable biomarker detection by ensuring validity through appropriate selection of test samples while reducing cost, effort, and time for analysis in single cell analysis or analysis of a group of a small number of cells.
    Type: Application
    Filed: June 24, 2015
    Publication date: July 12, 2018
    Inventors: Takahide YOKOI, Masataka SHIRAI
  • Publication number: 20180074006
    Abstract: A first tank 102a and a second tank 102b that can contain a solution containing an electrolyte; a thin membrane 103 that has a nanopore 104 and separates the first tank and the second tank as a partition; a first electrode 105 provided in the first tank; a second electrode 106 provided in the second tank; and a measurement system 109 that is connected to the first electrode and the second electrode and measures an ion current flowing through the nanopore, are provided. At least one electrode of the first electrode 105 and the second electrode 106 is made of a material containing a 1st group element, silver, and a 17th group element at least in an electrode surface part that is in contact with the solution.
    Type: Application
    Filed: May 11, 2015
    Publication date: March 15, 2018
    Inventors: Yusuke GOTO, Takahide YOKOI
  • Patent number: 9714449
    Abstract: A nucleic acid amplification method includes ligating a double-stranded adapter (20) containing adapter DNA strands capable of forming a folded structure to a double-stranded DNA (1, 2) containing a target DNA sequence (1) to prepare a cyclic DNA template composed of double-stranded DNA containing a nick (5). A 3?-end elongation reaction is performed using a strand-displacement DNA polymerase from the nick (5) as an origin, thereby producing a concatemer (29) in which a plurality of the target DNA sequences (1) and the adapter DNA strands capable of forming the folded structure are linked in series as a single-stranded DNA. The concatemer (29) contains a plurality of the target DNA sequences (1) suitable for nucleotide sequence analysis and has a folded shape such that it takes the form of a ball due to its folded structure.
    Type: Grant
    Filed: November 22, 2012
    Date of Patent: July 25, 2017
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Yoshiaki Yazawa, Takahide Yokoi, Chihiro Uematsu