Abstract: To provide a method and kit for measurement of a target substance with high detection sensitivity, provided is a method of measuring at least any one of the presence or absence, and a concentration, of a target substance in a sample liquid, the method including the steps of: (a) preparing a first particle that specifically binds to the target substance and contains a rare earth complex, and a second particle that has a larger average particle diameter than that of the first particle and specifically binds to the target substance; (b) mixing the sample liquid, the first particle, and the second particle to provide a mixed liquid; and (c) determining at least any one of the presence or absence, and the concentration, of the target substance from polarization anisotropy of the mixed liquid obtained in the step (b).

Abstract: Provided is a particle having high detection sensitivity in the detection of a substance to be measured, such as an antigen or an antibody, from a body. The particle includes a rare earth complex, wherein the particle has a particle size distribution of 0.1 or less in terms of polydispersity index (pdi) measured by dynamic light scattering, and wherein the particle has a hydrophilic polymer on a surface thereof.

Abstract: A measuring method includes a first sequence for acquiring a first result about the anisotropy by performing a dispensing step and a measuring step and for measuring concentration of the measurement object from the first result based on a relationship between the anisotropy and a dispensing amount of the fluorescent reagent dispensed in the dispensing step, and a second sequence for acquiring a second result about the anisotropy by performing the dispensing step and the measuring step one or more times after the first sequence and for measuring the concentration of the measurement object from the second result based on the relationship between the anisotropy measured in the measuring step and the dispensing amount of the fluorescent reagent dispensed in the dispensing step.

Abstract: There is provided a culture method and a culture device for efficiently producing cells and/or cell tissue suitable for medication applications. The culture method may include a first step of adhering the cells onto the electrode layer and culturing the cells thereon. The electrode layer may be coated with an oligopeptide including a terminal amino acid, a cell adhesive sequence, and an alternating sequence to be bound to the one end side of the cell-adhesive sequence. The alternating sequence may include a plurality of acidic amino acids and a plurality of basic amino acids that are alternately bound to each other one by one. The culture method may include a second step of applying, to the electrode layer having the cells adhered thereonto, an electrical potential inducing reductive desorption of the oligopeptide, to thereby detach the cells from the electrode layer.

Abstract: Provided are a culture method and a culture device for efficiently producing cells and/or cell tissue suitable for medication applications. A culture method of culturing cells adhered onto an electrode layer, the method including: a first step of adhering the cells onto the electrode layer and culturing the cells thereon, the electrode layer being coated with an oligopeptide including: a terminal amino acid; a cell adhesive sequence; and an alternating sequence to be bound to the one end side of the cell-adhesive sequence, the alternating sequence including a plurality of acidic amino acids and a plurality of basic amino acids, being alternately bound to each other one by one; and a second step of applying, to the electrode layer having the cells adhered thereonto, an electrical potential inducing reductive desorption of the oligopeptide, to thereby detach the cells from the electrode layer.