Patents by Inventor Takahiro SHIOYAMA
Takahiro SHIOYAMA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240008859Abstract: Provided is a porous body for capturing cancer cells, including a biocompatible inorganic material, the porous body for capturing cancer cells having biocompatibility and also having stability in a living body. The porous body for capturing cancer cells can be used for application related to cancer such as a treatment, a treatment assistance, a test, or a diagnosis.Type: ApplicationFiled: November 29, 2021Publication date: January 11, 2024Applicants: NIHON KOHDEN CORPORATION, NATIONAL UNIVERSITY CORPORATION TOKAI NATIONAL HIGHER EDUCATION AND RESEARCH SYSTEM, UNIVERSITY PUBLIC CORPORATION OSAKAInventors: Shinya NAGATA, Akane SUZUKI, Takahiro SHIOYAMA, Tadanori SUGIMOTO, Yukino SHINOZAKI, Masakazu SATSU, Chikara OHTSUKI, Jin NAKAMURA, Hideo AKIYOSHI, Hidetaka NISHIDA, Keiichiro MIE
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Patent number: 11553904Abstract: Provided is a cell isolation instrument capable of smoothly performing a plurality of steps associated with isolation of cells, including gripping of a biological tissue, transferring of the biological tissue, and isolating of the biological tissue. A container has an opening. A sealing member seals the opening. A pair of pinching parts is connected to the sealing member, and the pair of pinching parts opens or closes by being pressed.Type: GrantFiled: January 11, 2017Date of Patent: January 17, 2023Assignee: NIHON KOHDEN CORPORATIONInventors: Akane Suzuki, Takahiro Shioyama
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Patent number: 11313851Abstract: A device for measuring a tension of a cell structure containing muscle cells includes a first and a second gel adaptor holder. The first gel adaptor holder includes a frame member and a first gel holding portion which is disposed protrudingly from a part of an inner surface of the frame member for fixing one end of a gel. The second gel adaptor holder includes a second gel holding portion that fixes another end of the gel, and connecting members connected with the second gel holding portion. A kit including the device, a substrate and a gel forming cover. The substrate includes a pair of gel shaping convex parts fitted along the inner surface of the frame member. The gel forming cover includes a surface parallel to a gel contacting surface of the substrate, in order to form an upper surface of the gel.Type: GrantFiled: February 27, 2020Date of Patent: April 26, 2022Assignee: NIHON KOHDEN CORPORATIONInventors: Hirotsugu Kubo, Takahiro Shioyama, Yuki Kagawa, Yuto Hinata, Hodaka Makino
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Publication number: 20220002677Abstract: A transfer method includes a centrifugation step for applying centrifugal force in the direction of lamination to a laminate of a cell structure disposed on a temperature-responsive culture dish and a gel placed on the cell structure and a peeling step for maintaining the temperature at the lower critical solution temperature of the temperature-responsive culture dish or lower and peeling off the cell structure containing muscle cells from the temperature-responsive culture dish.Type: ApplicationFiled: July 2, 2021Publication date: January 6, 2022Applicant: NIHON KOHDEN CORPORATIONInventors: Hirotsugu KUBO, Takahiro SHIOYAMA, Yuki KAGAWA, Yuto HINATA, Tatsuya SHIMIZU, Daisuke SASAKI, Yuji HARAGUCHI
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Patent number: 11125670Abstract: A device for measuring a tension of a sheet-like tissue containing cardiomyocytes includes a first gel adapter holder having a frame member and a first gel holding member protruding toward a part of an inside face of the frame member for fixing one end of a film-like gel; and a second gel adapter holder having a second gel holding member for fixing the other end of the gel and a connection member connected to the second gel holding member. A kit includes the tension measuring device; a substrate having a pair of gel molding protruding members fitted along the inside face of the frame member; and a gel forming lid body having a face parallel to a gel contact face of the substrate so as to form an upper face of the gel. Further, a system for measuring the tension includes the tension measuring device.Type: GrantFiled: October 24, 2018Date of Patent: September 21, 2021Assignees: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITYInventors: Hirotsugu Kubo, Takahiro Shioyama, Yuki Kagawa, Tatsuya Shimizu, Daisuke Sasaki
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Publication number: 20200278342Abstract: A device for measuring a tension of a cell structure containing muscle cells includes a first and a second gel adaptor holder. The first gel adaptor holder includes a frame member and a first gel holding portion which is disposed protrudingly from a part of an inner surface of the frame member for fixing one end of a gel. The second gel adaptor holder includes a second gel holding portion that fixes another end of the gel, and connecting members connected with the second gel holding portion. A kit including the device, a substrate and a gel forming cover. The substrate includes a pair of gel shaping convex parts fitted along the inner surface of the frame member. The gel forming cover includes a surface parallel to a gel contacting surface of the substrate, in order to form an upper surface of the gel.Type: ApplicationFiled: February 27, 2020Publication date: September 3, 2020Applicant: NIHON KOHDEN CORPORATIONInventors: Hirotsugu Kubo, Takahiro Shioyama, Yuki Kagawa, Yuto Hinata, Hodaka Makino
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Patent number: 10633709Abstract: An apparatus and a method for diagnosing a tumor are provided. A histogram generating section generates a histogram from a result of a measurement of fluorescence intensity of a suspension produced from a target sample. The histogram indicates a relation between the fluorescence intensity and the number of cells. A determination section detects an S phase fluorescence intensity range corresponding to S phase cells based on the fluorescence intensity corresponding to a peak of the number of cells in the histogram, detects an abnormal fluorescence intensity range corresponding to tumor cells, based on a change in the number of cells in the S phase fluorescence intensity range, and performs a diagnosis of a tumor in the target sample based on an index of the number of cells in a range defined by excluding the abnormal fluorescence intensity range from the S phase fluorescence intensity range.Type: GrantFiled: April 19, 2017Date of Patent: April 28, 2020Assignee: NIHON KOHDEN CORPORATIONInventors: Takahiro Shioyama, Akane Suzuki, Yoshitaka Amano
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Publication number: 20190120742Abstract: A device for measuring a tension of a sheet-like tissue containing cardiomyocytes includes a first gel adapter holder having a frame member and a first gel holding member protruding toward a part of an inside face of the frame member for fixing one end of a film-like gel; and a second gel adapter holder having a second gel holding member for fixing the other end of the gel and a connection member connected to the second gel holding member. A kit includes the tension measuring device; a substrate having a pair of gel molding protruding members fitted along the inside face of the frame member; and a gel forming lid body having a face parallel to a gel contact face of the substrate so as to form an upper face of the gel. Further, a system for measuring the tension includes the tension measuring device.Type: ApplicationFiled: October 24, 2018Publication date: April 25, 2019Applicants: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITYInventors: Hirotsugu KUBO, Takahiro SHIOYAMA, Yuki KAGAWA, Tatsuya SHIMIZU, Daisuke SASAKI
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Patent number: 9970872Abstract: A cell analyzer includes: a measuring portion which measures cells that are nuclear stained; a displaying portion which displays a histogram of a fluorescence intensity by using a result of the measurement by the measuring portion; and a determining unit which obtains a number of strong-area cells that are distributed in an area where the fluorescence intensity is stronger than normal cells, and which determines a malignancy grade of cancer based on the number of strong-area cells and the histogram.Type: GrantFiled: August 25, 2011Date of Patent: May 15, 2018Assignee: Nihon Kohden CorporationInventors: Takahiro Shioyama, Akane Suzuki, Sunao Takeda
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Publication number: 20170306412Abstract: An apparatus and a method for diagnosing a tumor are provided. A histogram generating section generates a histogram from a result of a measurement of fluorescence intensity of a suspension produced from a target sample. The histogram indicates a relation between the fluorescence intensity and the number of cells. A determination section detects an S phase fluorescence intensity range corresponding to S phase cells based on the fluorescence intensity corresponding to a peak of the number of cells in the histogram, detects an abnormal fluorescence intensity range corresponding to tumor cells, based on a change in the number of cells in the S phase fluorescence intensity range, and performs a diagnosis of a tumor in the target sample based on an index of the number of cells in a range defined by excluding the abnormal fluorescence intensity range from the S phase fluorescence intensity range.Type: ApplicationFiled: April 19, 2017Publication date: October 26, 2017Inventors: Takahiro Shioyama, Akane Suzuki, Yoshitaka Amano
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Publication number: 20170196544Abstract: Provided is a cell isolation instrument capable of smoothly performing a plurality of steps associated with isolation of cells, including gripping of a biological tissue, transferring of the biological tissue, and isolating of the biological tissue. A container has an opening. A sealing member seals the opening. A pair of pinching parts is connected to the sealing member, and the pair of pinching parts opens or closes by being pressed.Type: ApplicationFiled: January 11, 2017Publication date: July 13, 2017Applicant: NIHON KOHDEN CORPORATIONInventors: Akane Suzuki, Takahiro Shioyama
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Patent number: 9658214Abstract: A method of analyzing cells, the method comprising: measuring a number of cells which are nuclear stained, in a to-be-determined tissue, and acquiring a histogram showing a fluorescence intensity based on a result of the measurement; analyzing the histogram, and acquiring data of predetermined parameters; comparing the data of the parameters with first thresholds predetermined for the parameters, to perform a first cancer determination for each of the parameters on the to-be-determined tissue; performing a scoring process for each of the parameters, on a result of the first cancer determination for each of the parameters, to calculate scores of the parameters; and combining the scores of the parameters with one another, thereby performing a second cancer determination on the to-be-determined tissue.Type: GrantFiled: July 19, 2013Date of Patent: May 23, 2017Assignee: NIHON KOHDEN CORPORATIONInventors: Takahiro Shioyama, Akane Suzuki, Hirotsugu Kubo, Sunao Takeda
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Patent number: 9448228Abstract: A cell analyzing apparatus includes: a histogram acquirer which is configured to perform measurement of a number of nuclear stained cells, and which, by using a result of the measurement, is configured to acquire a histogram indicating a fluorescence intensity; and an analysis controller which is configured to apply frequency analysis on data of the histogram acquired by the histogram acquirer, and which is configured to determine existence/nonexistence of cancer cells based on a result of the frequency analysis.Type: GrantFiled: June 13, 2013Date of Patent: September 20, 2016Assignee: NIHON KOHDEN CORPORATIONInventors: Sunao Takeda, Takahiro Shioyama, Akane Suzuki, Yo Kato, Nae Hinata
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Patent number: 9443127Abstract: A cell analyzing apparatus includes: a first histogram acquiring section which is configured to acquire a first histogram of fluorescence intensities by using a result of a measurement of a number of nuclear stained cells; a second histogram acquiring section which is configured to acquire a second histogram that is normalized based on a fluorescence intensity value indicating a maximum value of the first histogram; a determining section which is configured to determine whether cancer cells exist or not, based on one of the first histogram and the second histogram; and an outputting section which is configured to output a result of the determination performed by the determining section.Type: GrantFiled: September 17, 2014Date of Patent: September 13, 2016Assignee: Nihon Kohden CorporationInventors: Sunao Takeda, Hirotsugu Kubo, Takahiro Shioyama, Akane Suzuki, Kenichi Nomura, Yo Kato, Nae Hinata
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Patent number: 9163209Abstract: A pipette member to be attachable to a container capable of accommodating a tissue and a liquid, is used for isolating cells from the tissue by stirring, and includes: a cylinder member which includes a first opening that is to be accommodated in the container, and a second opening that is to be connected to a cell isolation apparatus, and in which a passage for communicating the first opening with the second opening is defined; and a filter member which is disposed inside the cylinder member, and which separates the passage into a first part that includes the first opening, and a second part that includes the second opening.Type: GrantFiled: July 3, 2012Date of Patent: October 20, 2015Assignee: NIHON KOHDEN CORPORATIONInventors: Takahiro Shioyama, Akane Suzuki
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Publication number: 20150078649Abstract: A cell analyzing apparatus includes: a first histogram acquiring section which is configured to acquire a first histogram of fluorescence intensities by using a result of a measurement of a number of nuclear stained cells; a second histogram acquiring section which is configured to acquire a second histogram that is normalized based on a fluorescence intensity value indicating a maximum value of the first histogram; a determining section which is configured to determine whether cancer cells exist or not, based on one of the first histogram and the second histogram; and an outputting section which is configured to output a result of the determination performed by the determining section.Type: ApplicationFiled: September 17, 2014Publication date: March 19, 2015Applicant: Nihon Kohden CorporationInventors: Sunao TAKEDA, Hirotsugu KUBO, Takahiro SHIOYAMA, Akane SUZUKI, Kenichi NOMURA, Yo KATO, Nae HINATA
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Patent number: 8882012Abstract: A cell isolation apparatus includes: a chamber body which includes a chamber into which tissue is to be introduced; an isolation member which is moved in the chamber and which is to collide with the tissue to isolate a cell; and a controlling portion which controls an operation of the isolation member in the chamber body.Type: GrantFiled: August 25, 2011Date of Patent: November 11, 2014Assignees: Nihon Kohden Corporation, Tokyo Women's Medical UniversityInventors: Yuji Haraguchi, Tatsuya Shimizu, Teruo Okano, Takahiro Shioyama, Akane Suzuki, Sunao Takeda
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Patent number: 8821815Abstract: A cell isolation instrument includes: a first container which has an opening in an upper portion; a filtration member which defines at least a part of a lower portion of the first container; an isolation member which is movably housed in the first container to collide with a tissue, thereby isolating cells; and a second container which houses the first container in a manner that the first container can be taken out.Type: GrantFiled: July 5, 2012Date of Patent: September 2, 2014Assignee: Nihon Kohden CorporationInventors: Takahiro Shioyama, Akane Suzuki, Teruo Okano, Tatsuya Shimizu, Yuji Haraguchi
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Publication number: 20140030728Abstract: A method of analyzing cells, the method comprising: measuring a number of cells which are nuclear stained, in a to-be-determined tissue, and acquiring a histogram showing a fluorescence intensity based on a result of the measurement; analyzing the histogram, and acquiring data of predetermined parameters; comparing the data of the parameters with first thresholds predetermined for the parameters, to perform a first cancer determination for each of the parameters on the to-be-determined tissue; performing a scoring process for each of the parameters, on a result of the first cancer determination for each of the parameters, to calculate scores of the parameters; and combining the scores of the parameters with one another, thereby performing a second cancer determination on the to-be-determined tissue.Type: ApplicationFiled: July 19, 2013Publication date: January 30, 2014Applicant: NIHON KOHDEN CORPORATIONInventors: Takahiro Shioyama, Akane Suzuki, Hirotsugu Kubo, Sunao Takeda
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Publication number: 20130337501Abstract: A cell analyzing apparatus includes: a histogram acquirer which is configured to perform measurement of a number of nuclear stained cells, and which, by using a result of the measurement, is configured to acquire a histogram indicating a fluorescence intensity; and an analysis controller which is configured to apply frequency analysis on data of the histogram acquired by the histogram acquirer, and which is configured to determine existence/nonexistence of cancer cells based on a result of the frequency analysis.Type: ApplicationFiled: June 13, 2013Publication date: December 19, 2013Inventors: Sunao Takeda, Takahiro Shioyama, Akane Suzuki, Yo Kato, Nae Hinata