Patents by Inventor Takaji Wakita

Takaji Wakita has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Nucleic Acid and Gene Derived from Novel HCV Strain and Replicon-Replicating Cell Using Said Gene
    Publication number: 20090035747
    Abstract: The present invention relates to a gene derived from a novel fulminant hepatitis C virus strain, an HCV replicon RNA with a high replication efficiency obtained using the gene, and an HCV replicon-replicating cell transfected with the replicon RNA. When the HCV replicon RNA and the HCV replicon-replicating cell of the present invention are used, HCV proteins can be continuously produced in a large amount.
    Type: Application
    Filed: September 16, 2004
    Publication date: February 5, 2009
    Inventors: Takaji Wakita, Takanobu Kato, Tomoko Date, Michiko Miyamoto
  • Nucleic Acid Construct Containing Fulllength Genome of Human Hepatitis C Virus, Recombinant Fulllength Virus Genome-Replicating Cells Having the Nucleic Acid Construct Transferred Thereinto and Method of Producing Hepatitis C Virus Particle
    Publication number: 20080220019
    Abstract: The present invention provides a method for replicating efficiently an RNA containing fulllength HCV genomic sequence and a method for producing HCV virus particles containing fulllength HCV replicon RNA or fulllength HCV genomic RNA by using a cell culture system. Further, the present invention relates to a method for producing hepatitis C virus particles which comprises culturing a cell, into which a replicon RNA comprising a nucleotide sequence comprising a fulllength genomic RNA sequence of hepatitis C virus of the genotype 2a, at least one selectable marker gene and/or at least one reporter gene and at least one IRES sequence or the fulllength genomic RNA of hepatitis C virus of the genotype 2a is introduced, and generating virus particles in the culture medium. Still further the present invention relates also to a hepatitis C vaccine and an antibody against hepatitis C virus particles.
    Type: Application
    Filed: February 21, 2005
    Publication date: September 11, 2008
    Applicants: Tokyo Metropolitan Organization for Medical Research, Toray Industries, Inc.
    Inventors: Takaji Wakita, Takanobu Kato, Tomoko Date, Michiko Miyamoto, Jun-ichi Tanabe, Saburo Sone
  • Nucleic Acid Construct Containing a Nucleic Acid Derived From the Genome of Hepatitis C Virus (Hcv) of Genotype 2A, and a Cell Having Such Nucleic Acid Construct Introduced Therein
    Publication number: 20080032323
    Abstract: The present invention relates to a replicon RNA comprising a nucleotide sequence at least containing the 5? untranslated region, the nucleotide sequence encoding NS3 protein, NS4A protein, NS4B protein, NS5A protein and NS5B protein, and the 3? untranslated region on the genomic RNA of hepatitis C virus of genotype 2a.
    Type: Application
    Filed: November 25, 2003
    Publication date: February 7, 2008
    Applicants: TORAY INDUSTRIES, INC, TOKYO METROPOLITAN ORGANIZATION FOR MEDICAL RESEARCH
    Inventors: Takaji Wakita, Takanobu Kato, Tomoko Date
  • Hepatitis C virus vaccine
    Patent number: 6831169
    Abstract: Nucleic acid molecule that comprise an incomplete hepatitis C viral genome are provided. Pharmaceutical compositions that contain nucleic acid molecules comprising an incomplete hepatitis C viral genome including a nucleotide sequence encoding a complete hepatitis C core protein operably linked to regulatory elements functional in human cells are provided. Methods of immunizing individuals susceptible to or infected by hepatitis C virus comprising the step of administering such pharmaceutical compositions are provided.
    Type: Grant
    Filed: May 1, 2001
    Date of Patent: December 14, 2004
    Assignee: The General Hospital Corporation
    Inventors: Catherine J. Pachuk, Jack Wands, Takaji Wakita, Vincent R. Zurawski, Jr., Leslie R. Coney
  • Hepatitis C virus vaccine
    Publication number: 20020161218
    Abstract: Nucleic acid molecule that comprise an incomplete hepatitis C viral genome including specifically disclosed DNA sequences are disclosed. Pharmaceutical compositions that contain nucleic acid molecules comprising an incomplete hepatitis C viral genome including a nucleotide sequence encoding a complete hepatitis C core protein operably linked to regulatory elements functional in human cells are disclosed.
    Type: Application
    Filed: May 1, 2001
    Publication date: October 31, 2002
    Applicant: Apollon, Inc.
    Inventors: Catherine J. Pachuk, Jack Wands, Takaji Wakita, Vincent R. Zurawski, Leslie R. Coney
  • Hepatitis C animal model
    Patent number: 6429355
    Abstract: A hepatitis type C animal model into which cDNA derived from hepatitis C virus has been introduced. This animal model is useful for clarification of an onset mechanism of hepatitis C and as well as for development of means for treating the disease.
    Type: Grant
    Filed: September 14, 2000
    Date of Patent: August 6, 2002
    Assignees: Tokyo Metropolitan Institute of Medical Science, Chugai Seiyaku Kabushiki Kaisha
    Inventors: Michinori Kohara, Takaji Wakita, Hiromichi Yonekawa, Choji Taya, Izumu Saito
  • Hepatitis C virus vaccine
    Patent number: 6235888
    Abstract: Nucleic acid molecule that comprise an incomplete hepatitis C viral genome including specifically disclosed DNA sequences are disclosed. Pharmaceutical compositions that contain nucleic acid molecules comprising an incomplete hepatitis C viral genome including a nucleotide sequence encoding a complete hepatitis C core protein operably linked to regulatory elements functional in human cells are disclosed. Methods of immunizing individuals susceptible to or infected by hepatitis C virus comprising the step of administering such pharmaceutical compositions are disclosed.
    Type: Grant
    Filed: June 5, 1997
    Date of Patent: May 22, 2001
    Assignees: The General Hospital Corporation, Apollon, Inc.
    Inventors: Catherine J. Pachuk, Jack Wands, Takaji Wakita, Vincent R. Zurawski, Jr., Leslie R. Coney
  • Transgenic mouse hepatitis C virus model comprising a hepatitis C viral fragment
    Patent number: 6201166
    Abstract: Transgenic mice are described which serve as a model for Hepatitis C infection. The transgenic mice contain, in their germline and somatic cells, the Hepatitis C viral fragment CN2, N24 or CR under the control of a Cre-loxP switch-expression system. Administration of Cre to the mice results in a phenotype of increased serum GTP levels, emergence of acidophilic bodies in the liver, exfoliation of hepatic cells, hypertrophy and hyperplasia of Kupffer's cells, and conglomeration of lymphocytes.
    Type: Grant
    Filed: January 21, 1999
    Date of Patent: March 13, 2001
    Assignee: Tokyo Metropolitan Institute of Medical Science
    Inventors: Michinori Kohara, Takaji Wakita, Hiromichi Yonekawa, Choji Taya, Izumu Saito
  • Chimeric hepatitis B/hepatitis C virus vaccine
    Patent number: 6025341
    Abstract: Nucleic acid molecule that comprise an incomplete hepatitis C and hepatitis B viral genome including specifically disclosed DNA sequences are disclosed. Pharmaceutical compositions that contain nucleic acid molecules comprising an incomplete hepatitis C and hepatitis B viral genome including a nucleotide sequence encoding a complete hepatitis C core protein and hepatitis B S gene protein operably linked to regulatory elements functional in human cells are disclosed. Methods of immunizing individuals susceptible to or infected by hepatitis B virus and/or hepatitis C virus comprising the step of administering such pharmaceutical compositions are disclosed.
    Type: Grant
    Filed: May 12, 1997
    Date of Patent: February 15, 2000
    Assignee: The General Hospital Corporation
    Inventors: Jack R. Wands, Katsutoshi Tokushige, Takaji Wakita