Patents by Inventor Takakazu Endo
Takakazu Endo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 6495716Abstract: Crystals of [S,S]-ethylenediamine-N,N′-disuccinic acid which have a bulk density of 0.45 to 1.2 g/cm3, and a process for obtaining these crystals comprising the steps of adjusting an aqueous solution of an [S,S]-ethylenediamine-N,N′-disuccinic acid metal salt to a temperature of 40 to 80° C. and a pH of 1.9 to 4.5, and cooling the solution to a temperature below 40° C. over a period of 0.2 to 10 hours while supplying a mineral acid so as to maintain the pH at 1.9 to 4.5 to precipitate [S,S]-ethylenediamine-N,N′-disuccinic acid in a high yield.Type: GrantFiled: February 27, 2002Date of Patent: December 17, 2002Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Hiroyasu Banba, Shigeho Tanaka, Kiyonobu Niwa, Takakazu Endo
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Patent number: 6414189Abstract: Crystals of [S,S]-ethylenediamine-N,N′-disuccinic acid which have a bulk density of 0.45 to 1.2 g/cm3, and a process for obtaining these crystals comprising the steps of adjusting an aqueous solution of an [S,S]-ethylenediamine-N,N′-disuccinic acid metal salt to a temperature of 40 to 80° C. and a pH of 1.9 to 4.5, and cooling the solution to a temperature below 40° C. over a period of 0.2 to 10 hours while supplying a mineral acid so as to maintain the pH at 1.9 to 4.5 to precipitate [S,S]-ethylenediamine-N,N′-disuccinic acid in a high yield.Type: GrantFiled: May 18, 2000Date of Patent: July 2, 2002Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Hiroyasu Banba, Shigeho Tanaka, Kiyonobu Niwa, Takakazu Endo
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Publication number: 20020082449Abstract: Crystals of [S,S]-ethylenediamine-N,N′-disuccinic acid which have a bulk density of 0.45 to 1.2 g/cm3, and a process for obtaining these crystals comprising the steps of adjusting an aqueous solution of an [S,S]-ethylenediamine-N,N′-disuccinic acid metal salt to a temperature of 40 to 80° C. and a pH of 1.9 to 4.5, and cooling the solution to a temperature below 40° C. over a period of 0.2 to 10 hours while supplying a mineral acid so as to maintain the pH at 1.9 to 4.5 to precipitate [S,S]-ethylenediamine-N,N′-disuccinic acid in a high yield.Type: ApplicationFiled: February 27, 2002Publication date: June 27, 2002Applicant: Mitsubishi Rayon co., Ltd.Inventors: Hiroyasu Banba, Shigeho Tanaka, Kiyonobu Niwa, Takakazu Endo
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Patent number: 6261798Abstract: The present invention relates to a method of producing [S,S]-ethylenediamine-N,N′-disuccinate wherein a microorganism having malate isomerase activity or matter processed therefrom and a microorganism having ethylenediamine-N,N′-disuccinate ethylenediamine lyase activity or matter processed therefrom are allowed to act on a substrate solution containing maleic acid, maleic anhydride, or a maleic acid salt, and ethylenediamine, in the presence of at least one metal ion selected from the group consisting of alkaline earth metals, iron, zinc, copper, nickel, aluminum, titanium and manganese. The present invention enables to accumulate [S,S]-ethylenediamine-N,N′-disuccinate in a higher yield and at a high concentration within a reaction system using maleic acid as a raw material.Type: GrantFiled: June 22, 2000Date of Patent: July 17, 2001Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Osamu Kato, Makoto Kaneko, Takakazu Endo
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Patent number: 6238896Abstract: The present invention relates to a method for preparing a malonic acid monoester represented by Formula (II): HOOCCH2COOR (II) wherein R is alkenyl, aryl, aralkyl or C1-20 alkyl, comprising treating a cyanoacetic acid ester represented by Formula (I): NCCH2COOR (I) wherein R is defined in Formula (II), with a culture, cells or a product from treated cells of a microorganism belonging to the genus Corynebacterium, Gordona or Rhodococcus and having nitrilase activity to thereby hydrolyze the cyanoacetic acid ester.Type: GrantFiled: August 20, 1999Date of Patent: May 29, 2001Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Eiji Ozaki, Kanehiko Enomoto, Takakazu Endo
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Patent number: 6136573Abstract: A process for producing an [S,S]-ethylenediamine-N,N'-disuccinic acid alkali metal salt which comprises reacting fumaric acid with ethylenediamine in an aqueous medium in the presence of an ion of at least one metal selected from the group consisting of alkaline earth metals and transition metals by the action of ethylenediamine-disuccinic acid ethylenediamine lyase of microorganism origin, adding an alkali hydroxide to a reaction product mixture thereby to separate and recover the metal ion as an insoluble precipitate, and obtaining an [S,S]-ethylenediamine-N,N'-disuccinic acid alkali metal salt.Type: GrantFiled: December 17, 1999Date of Patent: October 24, 2000Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Makoto Kaneko, Takakazu Endo, Toshihiko Fukuda, Mami Kato
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Patent number: 6103508Abstract: This invention relates to a method for removing fumarase activity from a microorganism or processed product thereof having ethylenediamine-N,N'-disuccinic acid ethylenediamine lyase activity, which includes treating the microorganism or processed product thereof with an aqueous alkaline solution at a pH of 8.0 to 10.5 in the presence of at least one salt with a concentration of 5 mM to 1000 mM. The salt is preferably selected from the group consisting of sodium, potassium, ammonium and C.sub.2-6 alkanediamine salts of boric acid, phosphoric acid, hydrochloric acid, sulfuric acid, acetic acid, oxalic acid, fumaric acid, maleic acid and ethylenediamine-N,N'-disuccinic acid, and mixtures thereof.Type: GrantFiled: October 28, 1998Date of Patent: August 15, 2000Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Mami Kato, Makoto Kaneko, Takakazu Endo
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Patent number: 5981238Abstract: A process for producing an optically active aminopolycarboxylic acid, such as S,S-ethylenediamine-N,N'-disuccinic acid, from a mixture of a diamine, such as ethylenediamine, with fumaric acid using a microorganism having a lyase activity, wherein at least one metal ion selected from the group consisting of an alkaline earth metal, iron, zinc, copper, nickel, aluminum, titanium and manganese is added to the reaction system. According to this process, aminopolycarboxylic acids, such as S,S-ethylenediamine-N,N'-disuccinic acid, or metal complexes thereof, can be appropriately and efficiently produced while improving the reaction yield.Type: GrantFiled: April 30, 1997Date of Patent: November 9, 1999Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Makoto Kaneko, Yoshihiro Hashimoto, Takakazu Endo, Mami Kato, Wataru Mizunashi
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Patent number: 5714357Abstract: A biological process for predominantly producing an optically active .alpha.-hydroxycarboxylic acid having a phenyl group directly from a racemic .alpha.-hydroxynitrile or a mixture of an aldehyde corresponding to the nitrile and prussic acid as a substrate is disclosed, comprising reacting a microorganism belonging to the genus Rhodococcus, Alcaligenes, Brevibacterium or Pseudomonas with the substrate in a neutral to basic aqueous medium. A desired optically active .alpha.-hydroxycarboxylic acid having a phenyl group can be obtained quantitatively at a high optical purity.Type: GrantFiled: December 12, 1996Date of Patent: February 3, 1998Assignee: Nitto Chemical Industry Co., Ltd.Inventors: Yoshihiro Hashimoto, Takakazu Endo, Koji Tamura, Yuji Hirata, Etsuko Kobayashi
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Patent number: 5707836Abstract: A method of producing an optically active amino acid is disclosed which comprises converting a mixture of an amino group-containing compound, e.g., an alkanediamine, and fumaric acid into an optically active amino acid by the action of a microorganism. The method is useful in industrially producing an optically active amino acid from the inexpensive starting materials, i.e., fumaric acid and an amino compound, under mild conditions of ordinary temperature and ordinary pressure.Type: GrantFiled: March 7, 1996Date of Patent: January 13, 1998Assignee: Nitto Chemical Industry Co., Ltd.Inventors: Takakazu Endo, Yoshihiro Hashimoto, Rikiya Takahashi
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Patent number: 5705382Abstract: Cells having enzyme activity, and the enzyme activity thereof, are preserved for a prolonged period of time, as a suspension of microbial cells or as a suspension of immobilized cells in particles, in an aqueous medium that is a neutral or weakly basic aqueous solution of inorganic salts, having a molarity ranging from 100 mM to the saturation concentration of the inorganic salts. Preferably, the microbial cells are cells containing the enzyme, nitrile hydratase or nitrilase, such as Gordona terrae or Rhodococcus rhodochrous, and the inorganic salts are phosphates, borates, sulfates, sulfites or hydrochlorides. The present invention provides an industrially useful method for preserving a large quantity of cells or immobilized cells in particles having nitrile hydratase or nitrilase enzyme activity for a prolonged period of time (e.g., 300 days) without cell lysis or enzyme deterioration even at room temperature.Type: GrantFiled: October 12, 1995Date of Patent: January 6, 1998Assignee: Nitto Chemical Industry Co., Ltd.Inventors: Takakazu Endo, Toshiaki Doi, Koji Tamura, Yuji Hirata, Kouzo Murao
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Patent number: 5580765Abstract: A biological process for predominantly producing an optically active .alpha.-hydroxycarboxylic acid having a phenyl group directly from a racemic .alpha.-hydroxynitrile or a mixture of an aldehyde corresponding to the nitrile and prussic acid as a substrate is disclosed, comprising reacting a microorganism belonging to the genus Gordona with the substrate in a neutral to basic aqueous medium. A desired optically active .alpha.-hydroxycarboxylic acid having a phenyl group can be obtained quantitatively at a high optical purity.Type: GrantFiled: February 3, 1994Date of Patent: December 3, 1996Assignee: Nitto Chemical Industry Co., Ltd.Inventors: Yoshihiro Hashimoto, Takakazu Endo, Koji Tamura, Yuji Hirata
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Patent number: 5326702Abstract: A process for biologically producing an .alpha.-hydroxyamide or an .alpha.-hydroxy acid represented by formula (III) ##STR1## wherein R represents a substituted or unsubstituted alkyl group, a substituted or unsubstituted alkenyl group, a substituted or unsubstituted cycloalkyl group, a substituted or unsubstituted alkoxy group, a substituted or unsubstituted aryl group, a substituted or unsubstituted aryloxy group or a substituted or unsubstituted and saturated or unsaturated heterocyclic group; and X represents an amido group or a carboxyl group, comprising reacting an .alpha.-hydroxynitrile represented by formula (I): ##STR2## wherein R is as defined above, or a mixture of an aldehyde represented by formula (II):R--CHO (II)wherein R is as defined above, and hydrogen cyanide with a microorganism capable of producing such an amide or acid from the corresponding .alpha.-hydroxynitrile is disclosed, in which the reaction system contains a sulfite ion, a disulfite ion or a dithionite ion.Type: GrantFiled: November 14, 1991Date of Patent: July 5, 1994Assignee: Nitto Chemical Industry Co., Ltd.Inventors: Takakazu Endo, Tomohide Yamagami, Koji Tamura
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Patent number: 5296373Abstract: A process for treating R,S-mandelonitrile or a derivative thereof or a mixture of benzaldehyde or a derivative thereof, and; hydrogen cyanide, with a microorganism belonging to the genus Rhodococcus sp. HT29-7 (FERM BP-3857) in an almost neutral or basic aqueous medium to thereby directly produce a predominant amount of R(-)-mandelic acid or a derivative thereof, whereby the racemic R,S-mandelonitrile or a derivative thereof or benzaldehyde or a derivative thereof and hydrogen cyanide can be directly converted into R(-)-mandelic acid or a derivative thereof at a ratio of about 80 to 100% and at an optical purity of almost 100%.sub.ee without performing any optical resolution.Type: GrantFiled: June 25, 1992Date of Patent: March 22, 1994Assignee: Nitto Chemical Industry Co., Ltd.Inventors: Takakazu Endo, Koji Tamura
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Patent number: 5238827Abstract: A process for preparing glycine from glycinonitrile by reacting glycinonitrile with a hydrolase produced by a microorganism, wherein the microorganism is a member of a genus selected from the group consisting of Rhodococcus, Arthrobacter, Caseobacter, Pseudomonas, Enterobacter, Acinetobacter, Alcaliqenes, and Streptomyces. The process involves no by-production, and the microorganism has high hydrolizing activity to convert glycinonitrile to glycine.Type: GrantFiled: March 29, 1991Date of Patent: August 24, 1993Assignee: Nitto Chemical Industry Co., Ltd.Inventors: Hitoshi Shimizu, Chiharu Fujita, Takakazu Endo, Ichiro Watanabe
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Patent number: 5234826Abstract: A process for preparing an optically active lactic acid comprising subjecting DL-lactonitrile or acetaldehyde and prussic acid to the action of a microorganism belonging to the genus Enterobacter, Arthrobacter, Caseobacter, Brevibacterium, Aureobacterium, Escherichia, Micrococcus, Streptomyces, Flavobacterium, Aeromonas, Nocardia, Mycoplana, Cellulomonas, Erwinia, Candida, Pseudomonas, Rhodococcus, Bacillus, Alcaligenes, Corynebacterium, Microbacterium or Obsumbacterium, or treated microbial cells thereof, which the microorganism is capable of stereospecifically hydrolyzing a nitrile in a polar solvent. D-Lactic acid or L-lactic acid can be produced directly in a predominant proportion from the starting materials.Type: GrantFiled: August 15, 1991Date of Patent: August 10, 1993Assignee: Nitto Chemical Industry Co., Ltd.Inventors: Tomohide Yamagami, Etsuko Kobayashi, Takakazu Endo
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Patent number: 5223416Abstract: A process for the predominantly producing R(-)-mandelic acid or a derivative thereof which comprises subjecting (i) R,S-mandelonitrile or a derivative thereof, or (ii) a mixture of prussic acid and benzaldehyde or a derivative of benzaldehyde to the action of a microorganism selected from the group consisting of the genus Aureobacterium, Pseudomonas, Caseobacter, Alcaligenes, Acinetobacter, Brevibacterium, Nocardia, and Bacillus or treated cells thereof, which the microorganism is capable of stereospecifically hydrolyzing a nitrile group of the R,S-mandelonitrile or a derivative thereof, in a neutral or basic aqueous reaction system to produce the R(-)-mandelic acid.Type: GrantFiled: March 29, 1991Date of Patent: June 29, 1993Assignee: Nitto Chemical Industry Co., Ltd.Inventors: Takakazu Endo, Koji Tamura
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Patent number: 5130235Abstract: A DNA which encodes polypeptide carrying two specific amino acid sequences and having nitrile hydratase activity; a method for producing nitrile hydratase by incubating a transformant made by the transformation with a recombinant DNA prepared by integrating the foregoing DNA into a vector in a medium and harvesting nitrile hydratase accumulated in the medium; and a method for producing amides by incubating the foregoing transformant and then converting nitriles into corresponding amides by the action of the obtained nitrile hydratase or by making a culture solution, isolates, treated cells or their immobilized products act upon nitriles to produce corresponding amides.Type: GrantFiled: June 28, 1989Date of Patent: July 14, 1992Assignee: Nitto Chemical Industry Co., Ltd.Inventors: Teruhiko Beppu, Sueharu Horinouchi, Osamu Ikehata, Takakazu Endo