Abstract: This invention provides a tool for recovering biological samples for realizing more simple and accurate clinical testing with the use of a trace amount of the blood sample, a kit comprising the same, and a method for recovering and separating biological samples using the same. Such tool comprises: a recovery container body composed of an upper container portion having a medium-accommodating portion, provided with an upper opening, for accommodating and conserving an absorption medium to be impregnated with the recovered biological samples and a lower container portion for accommodating the biological samples separated from the medium; and a lid for closing the upper opening of the medium-accommodating portion, the bottom of such medium-accommodating portion being provided with an open area in communication with the lower container portion.