Abstract: Provided are a novel DNA which encodes a selection marker appropriate for the transformation system of filamentous fungi of the genus Monascus and for expression of a recombinant DNA, and a novel DNA which functions as a promoter and a terminator. There is also provided a novel transformation system of filamentous fungi of the genus Monascus, in which filamentous fungi of the genus Monascus is transformed with a vector comprising the DNA encoding the above selection marker and the DNA encoding a desired protein in addition to promoter and terminator sequences to obtain a transformant; culturing the transformant; and isolating at high levels the expression product of the recombinant DNA.