Abstract: The present invention provides a fluidic device, an exosome analysis method, a biomolecule analysis method, and a biomolecule detection method, which can analyze even the content of an exosome in a series of flows by introducing a sample into the device. A fluidic device of the present invention is a fluidic device which detects a biomolecule contained in an exosome in a sample, and includes: an exosome purification unit which has a layer modified with a compound having a hydrophobic chain and a hydrophilic chain; a biomolecule purification unit; a biomolecule detection unit; a first flow path which connects the exosome purification unit to the biomolecule purification unit; and a second flow path which connects the biomolecule purification unit to the biomolecule detection unit.

Abstract: A method for detecting a target nucleic acid, comprising: (a) contacting a nucleic acid sample comprising a target nucleic acid, comprising a first portion and a second portion, with: (i) a detection probe, wherein the detection probe is labeled with a labeling substance and comprises a nucleic acid sequence that forms a stem-loop structure and having a 5? protruding end or a 3? protruding end that is capable of hybridizing to the second portion, and (ii) a capture probe comprising a nucleic acid sequence capable of hybridizing to the first portion, wherein the capture probe is immobilized to a substrate, under conditions to form a target nucleic acid-detection probe-capture probe complex by hybridizing the second portion to the detection probe and hybridizing the first portion to the capture probe; (b) ligating a first end of the detection probe with an end of the target nucleic acid and ligating a second end of the detection probe with an end of the capture probe; and (c) detecting the labeling substance of

Abstract: A method for detecting a target nucleic acid, comprising: (a) contacting a nucleic acid sample comprising a target nucleic acid, comprising a first portion and a second portion, with: (i) a detection probe, wherein the detection probe is labeled with a labeling substance and comprises a nucleic acid sequence that forms a stem-loop structure and having a 5? protruding end or a 3? protruding end that is capable of hybridizing to the second portion, and (ii) a capture probe comprising a nucleic acid sequence capable of hybridizing to the first portion, wherein the capture probe is immobilized to a substrate, under conditions to form a target nucleic acid-detection probe-capture probe complex by hybridizing the second portion to the detection probe and hybridizing the first portion to the capture probe; (b) ligating a first end of the detection probe with an end of the target nucleic acid and ligating a second end of the detection probe with an end of the capture probe; and (c) detecting the labeling substance of

Abstract: A method for detecting a nucleic acid includes (a) bringing a solution including a nucleic acid and a detection probe labeled with a labeling substance into contact with a solid phase on which a capture probe is immobilized, the capture probe having a first portion including a sequence capable of hybridizing with the nucleic acid, a second portion including a sequence capable of hybridizing with the detection probe, and a stem portion for forming a double strand; (b) hybridizing the nucleic acid with the first portion, hybridizing the detection probe with the second portion, and forming a nucleic acid-detection probe-capture probe complex on the solid phase; (c) ligating the nucleic acid and the detection probe; (d) eliminating binding between the capture probe and the detection probe that has not been ligated with the nucleic acid; and (e) detecting the labeling substance in the complex.

Abstract: The present invention provides a fluidic device, an exosome analysis method, a biomolecule analysis method, and a biomolecule detection method, which can analyze even the content of an exosome in a series of flows by introducing a sample into the device. A fluidic device of the present invention is a fluidic device which detects a biomolecule contained in an exosome in a sample, and includes: an exosome purification unit which has a layer modified with a compound having a hydrophobic chain and a hydrophilic chain; a biomolecule purification unit; a biomolecule detection unit; a first flow path which connects the exosome purification unit to the biomolecule purification unit; and a second flow path which connects the biomolecule purification unit to the biomolecule detection unit.

Abstract: A method for detecting a target nucleic acid, comprising: (a) contacting a nucleic acid sample comprising a target nucleic acid, comprising a first portion and a second portion, with: (i) a detection probe, wherein the detection probe is labeled with a labeling substance and comprises a nucleic acid sequence that forms a stem-loop structure and having a 5? protruding end or a 3? protruding end that is capable of hybridizing to the second portion, and (ii) a capture probe comprising a nucleic acid sequence capable of hybridizing to the first portion, wherein the capture probe is immobilized to a substrate, under conditions to form a target nucleic acid-detection probe-capture probe complex by hybridizing the second portion to the detection probe and hybridizing the first portion to the capture probe; (b) ligating a first end of the detection probe with an end of the target nucleic acid and ligating a second end of the detection probe with an end of the capture probe; and (c) detecting the labeling substance of

Abstract: A cross member is arranged so as to extend in a width direction of a vehicle body. The cross member has both ends fixed to side framework structures of the vehicle body. The cross member includes a base frame extending in the width direction and reinforcing frame parts formed in integral with the base frame to cover the circumference of the base frame within its limited ranges in the width direction of the vehicle body. The base frame and the reinforcing frame parts are made from the same resinous material or plural resinous materials belonging to engineering plastic system.

Abstract: A method of simply controlling the flow of fluid in a micro system without using a complicated value structure, comprising the steps of adding the substance transformed from sol-gel by stimulation to the fluid flowing through the micro flow passage of the micro system, and adding the stimulation to the fluid at a desired position on the micro flow passage to transform the fluid into gel for flow control.

Abstract: A micro system capable of setting an appropriate amount of stimulation being applied in order to control liquid flow in a channel. The micro system comprises micro-heaters (5b, 5c) for applying stimulation to liquid flowing through liquid channels (2b, 2c) formed in a plate (1) and controlling liquid flow by the stimulation from the micro-heaters (5b, 5c), and a means for electrically controlling the amount of stimulation being applied to the liquid from the micro-heaters (5b, 5c). An appropriate amount of stimulation can be set by electrically controlling the amount of stimulation being applied to the liquid from the micro-heaters (5b, 5c) through the control means.

Abstract: A cross member is arranged so as to extend in a width direction of a vehicle body. The cross member has both ends fixed to side framework structures of the vehicle body. The cross member includes a base frame extending in the width direction and reinforcing frame parts formed in integral with the base frame to cover the circumference of the base frame within its limited ranges in the width direction of the vehicle body. The base frame and the reinforcing frame parts are made from the same resinous material or plural resinous materials belonging to engineering plastic system.

Abstract: A method of simply controlling the flow of fluid in a micro system without using a complicated value structure, comprising the steps of adding the substance transformed from sol-gel by stimulation to the fluid flowing through the micro flow passage of the micro system, and adding the stimulation to the fluid at a desired position on the micro flow passage to transform the fluid into gel for flow control.