Abstract: An operation method of a hollow fiber membrane module is described, the operation method including: a filtration step of performing cross-flow filtration by supplying a feed to an outer surface side of a hollow fiber membrane, in which in the filtration step, a ratio of a viscosity ?f of the feed to a viscosity ?p of a filtrate satisfies a relation of ?f/?p?1.5, and a flow rate ratio of a flow rate vp of the filtrate to a flow rate vf of the feed satisfies a relation of 0.02?vp/vf?0.3.

Abstract: A system having ring generation device being configured for phase inverted synchronous operation with a primary converter device. The primary converter device is operable to supply power to at least one device. The primary converter device produces a first electromagnetic interference during operation. The ring generation device produces a second electromagnetic interference that cancels the first electromagnetic interference. The primary converter device and the ring generator device are synchronized and operate in an inverse phase with each other to generally cancel the electro-magnetic interference signal created by the primary converter device. A ring generation charging switch and a ring generation discharging switch generally turns on and off alternately which matches a charging and discharging of a primary switching node.

Abstract: The invention provides a method of producing a protein multimer-nucleic acid complex comprising a protein multimer and any one target component of the protein multimer. A nucleic acid encoding the target component is subjected to in vitro translation to provide a translation product containing a target component-nucleic acid complex of the target component and the nucleic acid encoding the target component. A nucleic acid encoding a non-target component constituting the protein multimer together with the target component is translated into the non-target component by adding the nucleic acid encoding the non-target component to the previously provided translation product to provide the non-target component. The non-target component then is associated with the target component contained in the target component-nucleic acid complex to form a protein multimer, thus affording the protein multimer-nucleic acid complex of the protein multimer and the nucleic acid encoding the target component.

Abstract: The polynucleotide construct of (1) or (2) below is used to perform ribosome display, CIS display and/or mRNA display in order to screen a Fab against an antigen of interest: (1) a polynucleotide construct which monocistronically comprises a ribosome-binding sequence, Fab first chain-coding sequence, linker peptide-coding sequence, Fab second chain-coding sequence and scaffold-coding sequence in this order, and further comprises at its 3?-end a structure necessary for maintaining a complex with the Fab encoded by itself; and (2) a polynucleotide construct which comprises a Fab first chain-expressing cistron and a Fab second chain-expressing cistron each containing a ribosome-binding sequence, a Fab first chain-coding sequence or Fab second chain-coding sequence, and a scaffold-coding sequence in this order, the first Fab-expressing cistron further comprising at its 3?-end a ribosome stall sequence, said Fab second chain-expressing cistron further comprising at its 3?-end a structure necessary for maintaining

Abstract: A system having ring generation device being configured for phase inverted synchronous operation with a primary converter device. The primary converter device is operable to supply power to at least one device. The primary converter device produces a first electromagnetic interference during operation. The ring generation device produces a second electromagnetic interference that cancels the first electromagnetic interference. The primary converter device and the ring generator device are synchronized and operate in an inverse phase with each other to generally cancel the electro-magnetic interference signal created by the primary converter device. A ring generation charging switch and a ring generation discharging switch generally turns on and off alternately which matches a charging and discharging of a primary switching node.

Abstract: The polynucleotide construct of (1) or (2) below is used to perform ribosome display, CIS display and/or mRNA display in order to screen a Fab against an antigen of interest: (1) a polynucleotide construct which monocistronically comprises a ribosome-binding sequence, Fab first chain-coding sequence, linker peptide-coding sequence, Fab second chain-coding sequence and scaffold-coding sequence in this order, and further comprises at its 3?-end a structure necessary for maintaining a complex with the Fab encoded by itself; and (2) a polynucleotide construct which comprises a Fab first chain-expressing cistron and a Fab second chain-expressing cistron each containing a ribosome-binding sequence, a Fab first chain-coding sequence or Fab second chain-coding sequence, and a scaffold-coding sequence in this order, the first Fab-expressing cistron further comprising at its 3?-end a ribosome stall sequence, said Fab second chain-expressing cistron further comprising at its 3?-end a structure necessary for maintaining

Abstract: A system having a noise cancellation converter being configured for phase inverted synchronous operation with a primary converter. The primary converter is operable to supply power to at least one device. The primary converter produces a first electromagnetic interference during operation. The noise cancellation converter is further configured with parasitic components matching parasitic components of the primary converter. The noise cancellation converter produces a second electromagnetic interference that is coupleable to the device. The second electromagnetic interference comprises frequency components having an inverted phase relative to frequency components of the first electromagnetic interference for reducing a sum of the first electromagnetic interference and the second electromagnetic interference during coupling to the device.

Abstract: According to the present invention, a composition possessing cell-free protein synthesis activity with reduced contaminating lipopolysaccharide, and a method for producing a protein using the same are provided. When ribosome display is performed using the composition and method for protein production of the present invention, the background that is caused by non-specific binding is reduced, so that a nucleic acid that encodes the desired polypeptide can be selected with high accuracy and high efficiency.

Abstract: A charging connector connecting device includes a cover that is movable between a closed position at which a connecting portion is closed and an open position at which the connecting portion is opened so that a second charging connector can be connected to the connecting portion; an elastic member that applies a biasing force to the cover so that the cover is positioned at an initial position between the closed position and the open position; and a pressing member configured to move with insertion of a first charging connector into the connecting portion so as to press the cover positioned at the initial position to thereby move the cover to the closed position.

Abstract: A charging connector connecting device includes a cover that is movable between a closed position at which a connecting portion is closed and an open position at which the connecting portion is opened so that a second charging connector can be connected to the connecting portion; an elastic member that applies a biasing force to the cover so that the cover is positioned at an initial position between the closed position and the open position; and a pressing member configured to move with insertion of a first charging connector into the connecting portion so as to press the cover positioned at the initial position to thereby move the cover to the closed position.

Abstract: The invention provides a method of producing a protein multimer-nucleic acid complex comprising a protein multimer and any one target component of the protein multimer. A nucleic acid encoding the target component is subjected to in vitro translation to provide a translation product containing a target component-nucleic acid complex of the target component and the nucleic acid encoding the target component. A nucleic acid encoding a non-target component constituting the protein multimer together with the target component is translated into the non-target component by adding the nucleic acid encoding the non-target component to the previously provided translation product to provide the non-target component. The non-target component then is associated with the target component contained in the target component-nucleic acid complex to form a protein multimer, thus affording the protein multimer-nucleic acid complex of the protein multimer and the nucleic acid encoding the target component.

Abstract: The invention provides a method of producing a protein multimer-nucleic acid complex comprising a protein multimer and any one target component of the protein multimer. A nucleic acid encoding the target component is subjected to in vitro translation to provide a translation product containing a target component-nucleic acid complex of the target component and the nucleic acid encoding the target component. A nucleic acid encoding a non-target component constituting the protein multimer together with the target component is translated into the non-target component by adding the nucleic acid encoding the non-target component to the previously provided translation product to provide the non-target component. The non-target component then is associated with the target component contained in the target component-nucleic acid complex to form a protein multimer, thus affording the protein multimer-nucleic acid complex of the protein multimer and the nucleic acid encoding the target component.

Abstract: A system having a noise cancelation converter being configured for phase inverted synchronous operation with a primary converter. The primary converter is operable to supply power to at least one device. The primary converter produces a first electromagnetic interference during operation. The noise cancelation converter is further configured with parasitic components matching parasitic components of the primary converter. The noise cancelation converter produces a second electromagnetic interference that is coupleable to the device. The second electromagnetic interference comprises frequency components having an inverted phase relative to frequency components of the first electromagnetic interference for reducing a sum of the first electromagnetic interference and the second electromagnetic interference during coupling to the device.

Abstract: According to the present invention, a composition possessing cell-free protein synthesis activity with reduced contaminating lipopolysaccharide, and a method for producing a protein using the same are provided. When ribosome display is performed using the composition and method for protein production of the present invention, the background that is caused by non-specific binding is reduced, so that a nucleic acid that encodes the desired polypeptide can be selected with high accuracy and high efficiency.

Abstract: An apparatus and system comprise a noise cancelation power converter being configured for phase inverted synchronous operation with respect to a primary power converter. The primary power converter is operable to supply power to at least one device. The primary power converter produces a first electromagnetic interference during operation to supply the power. The first electromagnetic interference is coupleable to the device. The noise cancelation power converter further is configured with parasitic components substantially matching parasitic components of the primary power converter. The noise cancelation power converter further produces a second electromagnetic interference that is coupleable to the device.

Abstract: According to the present invention, a composition possessing cell-free protein synthesis activity with reduced contaminating lipopolysaccharide, and a method for producing a protein using the same are provided. When ribosome display is performed using the composition and method for protein production of the present invention, the background that is caused by non-specific binding is reduced, so that a nucleic acid that encodes the desired polypeptide can be selected with high accuracy and high efficiency.

Abstract: A charge cable device includes: a cable having one end detachable from a power source or another charge cable device and an electric power line for supplying electricity to a vehicle from the electric power source or another charge cable device; a charge connector on the other end of the cable detachable from a charge inlet of the vehicle; a cable connector detachable from further another charge cable device to connect between further another charge cable device and the electric power line; and a controller. When the cable connector is not attached to further another charge cable device, the controller controls to supply electricity from the one end of the cable to the charge connector. When the cable connector is attached to further another charge cable device, the controller controls an electric power supply amount of electricity to the charge connector and to further another charge cable device.

Abstract: The present invention provides a method of producing a maturated oligonucleotide library, including a step of obtaining a terminal-modified product of a maturation target oligonucleotide library, including adding a tag sequence to the 5? terminus of the maturation target oligonucleotide library and an arrest sequence, which stalls translation elongation on a ribosome, to the 3? terminus of the maturation target oligonucleotide library, a step of transcribing the terminal-modified sequence product to give a transcript, and a step of in vitro translation for translating the transcript in vitro, wherein the maturation target oligonucleotide library is a random oligonucleotide library.

Abstract: A computing device including a power connector to interface with a power source, logic and power components on a current path from the power connector; the power connector providing electrical power to the logic and power components, and an inrush protection circuit on the current path between the power connector and the logic and power components, the inrush protection circuit including: a first power dissipation device and a second power dissipation device arranged in parallel on the current path, a feedback loop to detect an amount of current in the current path and to control the first power dissipation device to operate in a manner to dissipate detected inrush current, and a first time delay network in communication with the second power dissipation device and causing a delay for the second power dissipation device to transition to an on state, wherein during steady state.

Abstract: An apparatus and system comprise a noise cancelation power converter being configured for phase inverted synchronous operation with respect to a primary power converter. The primary power converter is operable to supply power to at least one device. The primary power converter produces a first electromagnetic interference during operation to supply the power. The first electromagnetic interference is coupleable to the device. The noise cancelation power converter further is configured with parasitic components substantially matching parasitic components of the primary power converter. The noise cancelation power converter further produces a second electromagnetic interference that is coupleable to the device.