Patents by Inventor Takashi NISHIKAZE

Takashi NISHIKAZE has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210391035
    Abstract: In a glycan structure analyzer according to one mode, a plurality of tabs for setting analysis conditions includes a dedicated tab for the setting of the conditions concerning a chemical modification specific to the linkage type of sialic acids, allowing a user to select major chemical modification agents. When one of those agents is selected, the mass change for each linkage type is automatically set. When using a chemical modification other than the preset ones, the user can input a numerical value of the amount of mass change for each linkage type. In an analysis, theoretical m/z values of a plurality of glycan structure candidates having different m/z values depending on the linkage type corresponding to one chemical modification are simultaneously compared with the m/z values of measured ion peaks.
    Type: Application
    Filed: May 23, 2019
    Publication date: December 16, 2021
    Applicant: SHIMADZU CORPORATION
    Inventors: Masaki MURASE, Takashi NISHIKAZE
  • Patent number: 11187630
    Abstract: A method for preparing an analytical sample for analyzing a glycan contained in a sample includes: performing a first reaction so that when sialic acid is linked to the glycan, sialic acid of a first linkage type is lactonized and modification different from lactonization is performed on sialic acid of a second linkage type different from the first linkage type; performing a second reaction to ring-open a lactone formed in the first reaction; and performing the first reaction again.
    Type: Grant
    Filed: August 22, 2019
    Date of Patent: November 30, 2021
    Assignee: SHIMADZU CORPORATION
    Inventor: Takashi Nishikaze
  • Patent number: 11105717
    Abstract: A method for preparing an analytical sample for analysis of a glycan that includes a lactone structure and is contained in a sample, includes: performing a first amidation reaction that amidates a sialic acid including the lactone structure through addition of a first amidation reaction solution to the sample, the first amidation reaction solution containing ammonia, an amine, or a salt thereof as a first nucleophilic agent that is reacted with the sialic acid including the lactone structure; and performing a second reaction that modifies at least a part of sialic acids not amidated in the first amidation reaction through a method different from permethylation.
    Type: Grant
    Filed: August 23, 2019
    Date of Patent: August 31, 2021
    Assignee: SHIMADZU CORPORATION
    Inventor: Takashi Nishikaze
  • Publication number: 20210208117
    Abstract: A glycopeptide analyzer that performs a structural analysis on glycoforms of a glycoprotein, including: a spectrum creator creating an MS/MS spectrum for each elution time based on data acquired by an LC/MS analysis of a sample containing glycopeptides originating from a target glycoprotein; a peptide mass calculator selecting a glycopeptide-related spectrum from a plurality of MS/MS spectra and calculating the mass of a peptide from the selected spectrum; a similarity determiner determining a similarity between the glycopeptide-related spectrum and each of the other MS/MS spectra; an elution-time range estimator estimating an elution-time range based on a distribution of the frequency of occurrence of an MS/MS spectrum for which a high level of similarity has been determined on a time axis; and a glycan composition estimator selecting an ion peak corresponding to a mass equal to or greater than a peptide mass and estimating a glycan composition based on the peak.
    Type: Application
    Filed: November 11, 2020
    Publication date: July 8, 2021
    Applicant: SHIMADZU CORPORATION
    Inventors: Masaki Murase, Takashi Nishikaze
  • Publication number: 20210164989
    Abstract: A method for preparing an analysis sample from a sample containing a glycan, includes: performing esterification reaction that subjects at least a part of a sialic acid included in the glycan to esterification other than lactonization; and performing amidation reaction that converts an esterified form of a sialic acid modified through the esterification into an amidated form through contacting the sample with an amidation reaction solution containing at least one compound selected from the group consisting of ammonia, amines, hydrazine, hydrazine derivatives, and hydroxyamine, and salts thereof to be reacted with the sialic acid modified through the esterification.
    Type: Application
    Filed: October 19, 2020
    Publication date: June 3, 2021
    Applicant: SHIMADZU CORPORATION
    Inventors: Takashi NISHIKAZE, Jun-ichi FURUKAWA, Hisatoshi HANAMATSU, Ikuko YOKOTA
  • Patent number: 10877043
    Abstract: Disclosed is a method of preparing a sample that is suitably used for analysis of a glycoprotein or glycopeptide. A first reaction is performed to modify or remove at least one primary amino group contained in a peptide moiety of a glycopeptide or glycoprotein. Thereafter, a second reaction is performed. The second reaction is a reaction capable of modifying a carboxy group of sialic acid of a sugar chain. After the second reaction, a derivative from ?2,3-linked sialic acid a derivative from ?2,6-linked sialic acid have different masses. Determination of the presence or absence of sialic acid in the sugar chain of a glycoprotein or glycopeptide and identification of the linkage type of sialic acid can be simply performed by mass spectrometry.
    Type: Grant
    Filed: December 19, 2017
    Date of Patent: December 29, 2020
    Assignee: SHIMADZU CORPORATION
    Inventor: Takashi Nishikaze
  • Patent number: 10712338
    Abstract: In the method of preparing a sample for analysis, a reaction is performed that produces different modified product depending on the sialic acid linkage type when a sialic acid is bound to a sugar chain of an analyte. In this reaction, an analyte containing a sugar chain, an amine containing two or more carbon atoms, and a dehydration-condensation agent are used. The sialic acid linkage type can be identified by analyzing the resulting sample with mass spectrometry etc. This method is applicable not only to free sugar chains but also to glycopeptides and glycoproteins.
    Type: Grant
    Filed: March 31, 2016
    Date of Patent: July 14, 2020
    Assignee: SHIMADZU CORPORATION
    Inventor: Takashi Nishikaze
  • Publication number: 20200109159
    Abstract: A method for preparing an analytical sample for analysis of a glycan contained in a sample includes: performing an amidation reaction that amidates a lactone structure included in the glycan through contacting the sample with a reaction solution that is basic; adding an acidic solution to the reaction solution after the reaction solution is subjected to the amidation reaction; and purifying the sample contained in the reaction solution after the acidic solution is added to the reaction solution by using a carrier for hydrophilic interaction chromatography.
    Type: Application
    Filed: October 2, 2019
    Publication date: April 9, 2020
    Applicant: SHIMADZU CORPORATION
    Inventor: Takashi NISHIKAZE
  • Publication number: 20200064234
    Abstract: A method for preparing an analytical sample for analyzing a glycan contained in a sample includes: performing a first reaction so that when sialic acid is linked to the glycan, sialic acid of a first linkage type is lactonized and modification different from lactonization is performed on sialic acid of a second linkage type different from the first linkage type; performing a second reaction to ring-open a lactone formed in the first reaction; and performing the first reaction again.
    Type: Application
    Filed: August 22, 2019
    Publication date: February 27, 2020
    Applicant: SHIMADZU CORPORATION
    Inventor: Takashi NISHIKAZE
  • Publication number: 20200064235
    Abstract: A method for preparing an analytical sample for analysis of a glycan that includes a lactone structure and is contained in a sample, includes: performing a first amidation reaction that amidates a sialic acid including the lactone structure through addition of a first amidation reaction solution to the sample, the first amidation reaction solution containing ammonia, an amine, or a salt thereof as a first nucleophilic agent that is reacted with the sialic acid including the lactone structure; and performing a second reaction that modifies at least a part of sialic acids not amidated in the first amidation reaction through a method different from permethylation.
    Type: Application
    Filed: August 23, 2019
    Publication date: February 27, 2020
    Applicant: SHIMADZU CORPORATION
    Inventor: Takashi NISHIKAZE
  • Publication number: 20190317101
    Abstract: Glycans having a branched structure are labeled with a labeling agent, such as 2-aminobenzoic acid, having one site that is easily negatively charged. At this time, reduction which is usually performed in labeling by reduction amination is not performed. A sample for mass spectrometry containing the labeled form of glycans thus obtained is prepared, and is subjected to MS/MS analysis in negative ion mode. In the MS/MS spectra obtained by the MS/MS analysis, peaks of E ions, D ions and the like which reflect the branched structure clearly appear. As a result, structural analysis of an entirety of the glycan including the branched structure can be easily performed.
    Type: Application
    Filed: November 7, 2016
    Publication date: October 17, 2019
    Applicant: SHIMADZU CORPORATION
    Inventor: Takashi NISHIKAZE
  • Publication number: 20190271617
    Abstract: A method for preparing a sample comprising a glycan includes: performing a lactonization reaction in which at least a part of sialic acids included in the glycan is lactonized; and performing an amidation reaction in which lactones of lactonized sialic acids are amidated through addition of an amidation reaction solution to the sample, the amidation reaction solution comprising at least one selected from the group consisting of ammonia, an amine, and a salt thereof that is reacted with the lactonized sialic acids.
    Type: Application
    Filed: February 28, 2019
    Publication date: September 5, 2019
    Applicant: SHIMADZU CORPORATION
    Inventors: Takashi NISHIKAZE, Hisatoshi HANAMATSU, Jun-ichi FURUKAWA
  • Publication number: 20190120793
    Abstract: Provided is a method for efficiently analyzing the structure of a sialylated glycan, including the linkage type of a sialic acid linked to a terminal of the glycan. After the sialic acid residues in a sample (glycan) to be analyzed are modified in a linkage-type-specific way (S2), a mass spectrum for the sample is obtained by mass spectrometry (S3). Based on the masses of the modified glycans estimated from the m/z values of the peaks observed on the mass spectrum, all possible monosaccharide compositions are exhaustively estimated under specific conditions including the kinds of monosaccharides and the range of the number of occurrences of each monosaccharide (S4). Monosaccharide composition candidates listed for each peak are narrowed down by using the mass difference between different modifications of a sialic acid residue generated by the linkage-type-specific modification (S5).
    Type: Application
    Filed: December 9, 2016
    Publication date: April 25, 2019
    Applicant: SHIMADZU CORPORATION
    Inventor: Takashi NISHIKAZE
  • Publication number: 20180180627
    Abstract: Disclosed is a method of preparing a sample that is suitably used for analysis of a glycoprotein or glycopeptide. A first reaction is performed to modify or remove at least one primary amino group contained in a peptide moiety of a glycopeptide or glycoprotein. Thereafter, a second reaction is performed. The second reaction is a reaction capable of modifying a carboxy group of sialic acid of a sugar chain. After the second reaction, a derivative from ?2,3-linked sialic acid a derivative from ?2,6-linked sialic acid have different masses. Determination of the presence or absence of sialic acid in the sugar chain of a glycoprotein or glycopeptide and identification of the linkage type of sialic acid can be simply performed by mass spectrometry.
    Type: Application
    Filed: December 19, 2017
    Publication date: June 28, 2018
    Applicant: SHIMADZU CORPORATION
    Inventor: Takashi NISHIKAZE
  • Publication number: 20180059094
    Abstract: In the method of preparing a sample for analysis, a reaction is performed that produces different modified product depending on the sialic acid linkage type when a sialic acid is bound to a sugar chain of an analyte. In this reaction, an analyte containing a sugar chain, an amine containing two or more carbon atoms, and a dehydration-condensation agent are used. The sialic acid linkage type can be identified by analyzing the resulting sample with mass spectrometry etc. This method is applicable not only to free sugar chains but also to glycopeptides and glycoproteins.
    Type: Application
    Filed: March 31, 2016
    Publication date: March 1, 2018
    Applicant: SHIMADZU CORPORATION
    Inventor: Takashi NISHIKAZE