Patents by Inventor Takashi Uemori
Takashi Uemori has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20180163263Abstract: Provided are: a method with which it is possible to suppress nucleic-acid amplification of non-target nucleic acids and to selectively nucleic-acid amplify a target nucleic acid in a sample in which a large amount of non-target nucleic acids and a rare target nucleic acid are present in mixture; a method for detecting at high sensitivity rare mutant genes, the method combining this nucleic-acid amplification method and a specific real-time method for detecting the target nucleic acid; and a composition and kit for this method.Type: ApplicationFiled: March 18, 2016Publication date: June 14, 2018Applicant: TAKARA BIO INC.Inventors: Takashi UEMORI, Takehiro SAGARA, Koichi INOUE
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Publication number: 20170260578Abstract: Provided are the following: a method, for improving reactivity of an acid synthesis reaction, comprising a step for adding an ?-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleoside triphosphate, and an ?-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ?-amino acid.Type: ApplicationFiled: May 23, 2017Publication date: September 14, 2017Applicant: TAKARA BIO INC.Inventors: Kiyoyuki MATSUMURA, Takashi UEMORI, Hiroyuki MUKAI
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Publication number: 20170253909Abstract: A polypeptide having a mismatch endonuclease activity of recognizing a mismatch and cleaving the mismatch; a mismatch-specific cleavage reaction using the polypeptide; a method for removing an error in a nucleic acid amplification reaction utilizing the polypeptide; a method for inhibiting the amplification of a nucleic acid comprising a specific nucleotide sequence during a nucleic acid amplification reaction; and a method for detecting a nucleic acid having a single-nucleotide polymorphism mutation utilizing the inhibition method.Type: ApplicationFiled: September 9, 2015Publication date: September 7, 2017Applicants: TAKARA BIO INC., KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, EDUCATIONAL CORPORATION KANSAI BUNRI SOUGOUGAKUENInventors: Takashi UEMORI, Yoshizumi ISHINO, Takehiro SAGARA, Sonoko ISHINO, Takeshi YAMAGAMI, Tsuyoshi SHIRAI
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Patent number: 9689013Abstract: Provided are the following: a method, for improving reactivity of a nucleic acid synthesis reaction, comprising a step for adding an ?-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleotide triphosphate, and an ?-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ?-amino acid.Type: GrantFiled: January 24, 2013Date of Patent: June 27, 2017Assignee: TAKARA BIO INC.Inventors: Kiyoyuki Matsumura, Takashi Uemori, Hiroyuki Mukai
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Patent number: 9670478Abstract: The present invention pertains to a method for modifying a nucleic acid contained in a sample comprising a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, comprising the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.Type: GrantFiled: August 31, 2016Date of Patent: June 6, 2017Assignee: TAKARA BIO INC.Inventors: Junko Yamamoto, Keiko Kubo, Takashi Uemori, Hiroyuki Mukai, Kiyozo Asada
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Publication number: 20160362676Abstract: The present invention pertains to: a method for modifying a nucleic acid contained in a sample, said method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, said method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.Type: ApplicationFiled: August 31, 2016Publication date: December 15, 2016Inventors: Junko YAMAMOTO, Keiko KUBO, Takashi UEMORI, Hiroyuki MUKAI, Kiyozo ASADA
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Patent number: 9458498Abstract: The present invention pertains to: a method for modifying a nucleic acid contained in a sample, the method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, the method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.Type: GrantFiled: January 20, 2012Date of Patent: October 4, 2016Assignee: TAKARA BIO INC.Inventors: Junko Yamamoto, Keiko Kubo, Takashi Uemori, Hiroyuki Mukai, Kiyozo Asada
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Patent number: 9447388Abstract: Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A737-A738-A739-A740-A741-A742-A743-A744-A745-” between the amino acid residue at position 736 and the amino acid residue at position 737, wherein: A737 is an amino acid residue having a non-polar aliphatic side chain; A738 is an amino acid residue having a non-polar aliphatic side chain; A739 is an amino acid residue having a positively charged side chain; A740 is an amino acid residue having a positively charged side chain; A741 is an amino acid residue having a non-polar aliphatic side chain; A742 is an amino acid residue having a non-polar aliphatic side chain; A743 is any given amino acid residue; A744 is an amino acid residue having a positively charged side chain; and A745 is an amino acid residue having a non-polar aliphatic side chain).Type: GrantFiled: July 12, 2012Date of Patent: September 20, 2016Assignees: KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, TAKARA BIO INC.Inventors: Yoshizumi Ishino, Takeshi Yamagami, Hiroaki Matsukawa, Takashi Uemori, Takehiro Sagara
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Patent number: 9422599Abstract: The present invention provides cold shock protein-containing compositions for improved DNA synthesis reactions with improved reactivity, methods for synthesizing DNA using such compositions, kits for use in such methods, and DNA compositions yielded by such methods. The present invention further provides cold shock protein-containing compositions for the identification of endoribonuclease cleavage sites, methods for identifying endoribonuclease cleavage sites using such compositions, and kits for use in such methods.Type: GrantFiled: March 2, 2009Date of Patent: August 23, 2016Assignee: Rutgers, The State University of New JerseyInventors: Masayori Inouye, Sangita Phadtare, Ikunoshin Kato, Ling Zhu, Hiroyuki Mukai, Takashi Uemori, Kazue Nishiwaki
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Publication number: 20160017300Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: ApplicationFiled: March 13, 2014Publication date: January 21, 2016Applicant: TAKARA BIO INC.Inventors: Kiyoyuki MATSUMURA, Nariaki TAKATSU, Takashi UEMORI, Hiroyuki MUKAI
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Patent number: 9200314Abstract: A method for accurately and easily detecting a synthetic siRNA, for example, a siRNA in which the 3? end is DNA, and a kit used for the method are provided. The present invention relates to a method for detecting a siRNA in which the 3? end is DNA, comprising: (a) adding polydeoxyadenosine to the 3? DNA end of at least one strand of the siRNA to be detected to produce a polydeoxyadenosine-added RNA; (b) annealing a polydeoxythymidine primer having a tag sequence at its 5? side to the polydeoxyadenosine-added RNA and synthesizing DNA from the primer by a reverse transcription; and (c) detecting the DNA synthesized in (b).Type: GrantFiled: July 20, 2011Date of Patent: December 1, 2015Assignee: TAKARA BIO INC.Inventors: Satoshi Yanagi, Eiji Kobayashi, Takashi Uemori, Hiroyuki Mukai
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Patent number: 9169482Abstract: A method for synthesizing cDNA characterized by preparing a reaction solution that does not allow an endodeoxyribonuclease to show its activity, without thermal deactivation of the endodeoxyribonuclease or removal of the endodeoxyribonuclease, and carrying out a reverse transcription reaction, wherein the reaction solution contains a treated sample and a reverse transcriptase, the treated sample being formed by treating a sample comprising RNA and DNA with the endodeoxyribonuclease to degrade DNA in the sample. The method and the kit for synthesizing cDNA of the present invention are widely useful in genetic engineering fields.Type: GrantFiled: May 9, 2011Date of Patent: October 27, 2015Assignee: TAKARA BIO INC.Inventors: Yuko Nakabayashi, Takashi Uemori, Hiroyuki Mukai, Kiyozo Asada
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Publication number: 20140363849Abstract: Provided are the following: a method, for improving reactivity of a nucleic acid synthesis reaction, comprising a step for adding an ?-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleotide triphosphate, and an ?-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ?-amino acid.Type: ApplicationFiled: January 24, 2013Publication date: December 11, 2014Applicant: Takara Bio Inc.Inventors: Kiyoyuki Matsumura, Takashi Uemori, Hiroyuki Mukai
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Publication number: 20140322793Abstract: Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A737-A738-A739-A740-A741-A742-A743-A744-A745-” between the amino acid residue at position 736 and the amino acid residue at position 737, wherein: A737 is an amino acid residue having a non-polar aliphatic side chain; A738 is an amino acid residue having a non-polar aliphatic side chain; A739 is an amino acid residue having a positively charged side chain; A740 is an amino acid residue having a positively charged side chain; A741 is an amino acid residue having a non-polar aliphatic side chain; A742 is an amino acid residue having a non-polar aliphatic side chain; A743 is any given amino acid residue; A744 is an amino acid residue having a positively charged side chain; and A745 is an amino acid residue having a non-polar aliphatic side chain).Type: ApplicationFiled: July 12, 2012Publication date: October 30, 2014Applicants: TAKARA BIO INC., KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATIONInventors: Yoshizumi Ishino, Takeshi Yamagami, Hiroaki Matsukawa, Takashi Uemori, Takehiro Sagara
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Publication number: 20130295576Abstract: The present invention pertains to: a method for modifying a nucleic acid contained in a sample, said method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, said method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.Type: ApplicationFiled: January 20, 2012Publication date: November 7, 2013Inventors: Junko Yamamoto, Keiko Kubo, Takashi Uemori, Hiroyuki Mukai, Kiyozo Asada
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Publication number: 20130065281Abstract: A method for synthesizing cDNA characterized by preparing a reaction solution that does not allow an endodeoxyribonuclease to show its activity, without thermal deactivation of the endodeoxyribonuclease or removal of the endodeoxyribonuclease, and carrying out a reverse transcription reaction, wherein the reaction solution contains a treated sample and a reverse transcriptase, the treated sample being formed by treating a sample comprising RNA and DNA with the endodeoxyribonuclease to degrade DNA in the sample. The method and the kit for synthesizing cDNA of the present invention are widely useful in genetic engineering fields.Type: ApplicationFiled: May 9, 2011Publication date: March 14, 2013Applicant: TAKARA BIO INC.Inventors: Yuko Nakabayashi, Takashi Uemori, Hiroyuki Mukai, Kiyozo Asada
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Patent number: 8367328Abstract: A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3??5? exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase.Type: GrantFiled: October 15, 2008Date of Patent: February 5, 2013Assignee: Takara Bio Inc.Inventors: Kiyozo Asada, Takashi Uemori, Yoshimi Sato, Tomoko Fujita, Kazue Miyake, Osamu Takeda, Hiroyuki Mukai, Ikunoshin Kato
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Patent number: 8344105Abstract: A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.Type: GrantFiled: September 22, 2011Date of Patent: January 1, 2013Assignee: Takara Bio Inc.Inventors: Yoshimi Sato, Kazue Nishiwaki, Nana Shimada, Shigekazu Hokazono, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
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Publication number: 20120164654Abstract: A composition for a reverse transcription polymerase chain reaction, which comprises a thermostable DNA polymerase, a reverse transcriptase, a dye marker and a specific gravity-increasing agent; and a premix reagent for a one-step RT-PCR, which comprises the composition, is not frozen under usual storage conditions at ?20 to ?30° C. and has excellent handleability.Type: ApplicationFiled: August 27, 2010Publication date: June 28, 2012Applicant: TAKARA BIO INC.Inventors: Yuko Nakabayashi, Yoshimi Sato, Takashi Uemori, Hiroyuki Mukai
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Publication number: 20120083590Abstract: A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.Type: ApplicationFiled: September 22, 2011Publication date: April 5, 2012Applicant: Takara Bio Inc.Inventors: Yoshimi Sato, Kazue Nishiwaki, Nana Shimada, Shigekazu Hokazono, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato