Abstract: An object identification method capable of quickly and accurately identifying a virus or the like is provided. An object identification method according to an aspect of the present disclosure includes feeding an object dispersed in a solvent to a micro-channel, and applying an AC (Alternating Current) voltage to a measurement electrode provided at the micro-channel and measuring an AC characteristic of the object when the object passes through the micro-channel. Then, a combined impedance and a phase are determined by using the measured AC characteristic, and the object is identified by using the determined combined impedance and the phase.

Abstract: Provided are a method for etching a metal or metal oxide without using a reagent, etc., that affects the environment, a method for smoothing a surface of a metal or metal oxide on an atomic level, and a method for patterning on an atomic level. Etching of a metal or metal oxide, or smoothing of a surface of a metal or metal oxide is possible using ozone water in which only ozone is dissolved. Patterning can also be performed by providing a metal that does not dissolve in the ozone water as a resist on a metal or metal oxide that can be etched by ozone water in which only ozone is dissolved, and etching using the ozone water.

Abstract: A channel device including a nanosize channel through which single molecule flows, at least one electrode pair arranged near the nanosize channel, and an AC power source that applies an AC voltage to the electrodes. This channel device is useful for identifying molecules one by one. Furthermore, a channel device including a nanosize channel through which single molecule flows, a branching portion, and a plurality of branching channels, wherein (i) an electrode pair is arranged near the nanosize channel so as to sandwich the nanosize channel between the electrodes, or (ii) one electrode of the electrode pair is located near the nanosize channel, whereas the other is arranged near the branching channels. This channel device is useful for separating single molecule. The present channel device achieves identification or separation at an accuracy of 100% in principle. A sample treatment apparatus according to present invention includes a channel device, a measurement section, and an arithmetic processing section.

Abstract: Provided are a method for etching a metal or metal oxide without using a reagent, etc., that affects the environment, a method for smoothing a surface of a metal or metal oxide on an atomic level, and a method for patterning on an atomic level. Etching of a metal or metal oxide, or smoothing of a surface of a metal or metal oxide is possible using ozone water in which only ozone is dissolved. Patterning can also be performed by providing a metal that does not dissolve in the ozone water as a resist on a metal or metal oxide that can be etched by ozone water in which only ozone is dissolved, and etching using the ozone water.

Abstract: A method for adhering a major substrate to which nano sized concave and convex structures are formed without damaging the concave and convex structures can be used in a production method of a microfluidic device including nano sized fluidic channels. The substrates may be adhered under heat free and adhesive agent free environment by exciting the substrate surface including hard silicone resin with atmospheric plasma or vacuum ultraviolet light, then juxtaposing and pressurizing the surface of the hard silicone resin substrate and glass substrate. The silicone rubber composition is applied on the contact face of the cover substrate and then the silicone rubber composition is cured to form the silicone rubber layer and then the ultraviolet light is exposed under the condition that the surface of the major substrate being formed with concave and convex structures is contacted closely to the silicone rubber layer of the cover substrate.

Abstract: The invention is directed to methods for the propagation or cultivation of cells including preparing a cell culture substrate, wherein the cell culture substrate includes a substrate and a layer formed by surface modification. The layer includes a polymer containing an amino group. The polymer is produced by reacting a polymer represented by formula (II): with a polymer having at least one amino group, —NH2, capable of forming a Schiff base in a monomer of formula (II), thereby forming a polymer layer constituting the layer formed by surface modification. “n” in Formula (II) is 0 or a positive integer, and m is a positive integer. n and m represent the degree of polymerization. Formula (II) is formed by chemical vapor deposition of formyl[2.2]paracyclophane. The methods further include providing cells in a medium; inoculating the cells onto the cell culture substrate; and culturing the cells, wherein the cells adhere to the cell culture substrate.

Abstract: A method for adhering a major substrate to which nano sized concave and convex structures are formed without damaging the concave and convex structures can be used in a production method of a microfluidic device including nano sized fluidic channels. The substrates may be adhered under heat free and adhesive agent free environment by exciting the substrate surface including hard silicone resin with atmospheric plasma or vacuum ultraviolet light, then juxtaposing and pressurizing the surface of the hard silicone resin substrate and glass substrate. The silicone rubber composition is applied on the contact face of the cover substrate and then the silicone rubber composition is cured to form the silicone rubber layer and then the ultraviolet light is exposed under the condition that the surface of the major substrate being formed with concave and convex structures is contacted closely to the silicone rubber layer of the cover substrate.

Abstract: A channel device including a nanosize channel through which single molecule flows, at least one electrode pair arranged near the nanosize channel, and an AC power source that applies an AC voltage to the electrodes. This channel device is useful for identifying molecules one by one. Furthermore, a channel device including a nanosize channel through which single molecule flows, a branching portion, and a plurality of branching channels, wherein (i) an electrode pair is arranged near the nanosize channel so as to sandwich the nanosize channel between the electrodes, or (ii) one electrode of the electrode pair is located near the nanosize channel, whereas the other is arranged near the branching channels. This channel device is useful for separating single molecule. The present channel device achieves identification or separation at an accuracy of 100% in principle. A sample treatment apparatus according to present invention includes a channel device, a measurement section, and an arithmetic processing section.

Abstract: A cell culture substrate which is durable and which can be readily produced in commercial scale at a low cost, and its production method are provided. The cell culture substrate comprises a substrate and a layer formed by surface modification, which comprises a polymer containing amino group produced by reacting a polymer represented by the following formula (II): (wherein n is 0 or a positive integer, and m is a positive integer, the n and m representing degree of polymerization) formed by chemical vapor deposition of formyl[2.2]paracyclophane represented by the following formula (I): (wherein k is 0 or 1) with a polymer having at least one amino group (—NH2) capable of forming Schiff base in its monomer. The production method of the cell culture substrate comprises the step of synthesizing such polymer on the substrate.

Abstract: A cell culture substrate which is durable and which can be readily produced in commercial scale at a low cost, and its production method are provided. The cell culture substrate comprises a substrate and a layer formed by surface modification, which comprises a polymer containing amino group produced by reacting a polymer represented by the following formula (II): (wherein n is 0 or a positive integer, and m is a positive integer, the n and m representing degree of polymerization) formed by chemical vapor deposition of formyl[2.2]paracyclophane represented by the following formula (I): (wherein k is 0 or 1) with a polymer having at least one amino group (—NH2) capable of forming Schiff base in its monomer. The production method of the cell culture substrate comprises the step of synthesizing such polymer on the substrate.

Abstract: In order to be capable of a chemical reaction, analysis or the like wherein a small amount of samples is used, a reactor comprises a flat plate-like first substrate the inside of which is provided with a heating means; and a flat plate-like second substrate, which is placed on the top of the above-described first substrate, and on a surface thereof to be placed on the top of the above-described first substrate a flow channel having a predetermined contour has been defined.

Abstract: In order to be capable of a chemical reaction, analysis or the like wherein a small amount of samples is used, a reactor comprises a flat plate-like first substrate the inside of which is provided with a heating means; and a flat plate-like second substrate, which is placed on the top of the above-described first substrate, and on a surface thereof to be placed on the top of the above-described first substrate a flow channel having a predetermined contour has been defined.