Abstract: An enzyme solution for separating cell(s) which can lessen damage in separating a single cell or a mass of cells from a tissue or an organ, a method for separating cell(s) using the enzyme solution, and a method for separating pancreatic islet(s) using the enzyme solution. The enzyme solution for separating cell(s) is an enzyme solution containing a chloride ion channel inhibitor or having a chloride ion concentration of 10 mM or lower. By an enzymatic treatment using the enzyme solution for separating cell(s), a single cell or a mass of cells can be separated from a tissue or an organ. To separate pancreatic islet(s) from the pancreas, the enzyme is injected through the pancreatic duct to digest the pancreas.