Patents by Inventor Takayuki ARAZOE

Takayuki ARAZOE has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHOD FOR CONVERTING NUCLEIC ACID SEQUENCE OF CELL SPECIFICALLY CONVERTING NUCLEIC ACID BASE OF TARGETED DNA USING CELL ENDOGENOUS DNA MODIFYING ENZYME, AND MOLECULAR COMPLEX USED THEREIN
    Publication number: 20240117384
    Abstract: Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.
    Type: Application
    Filed: December 14, 2023
    Publication date: April 11, 2024
    Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Keiji NISHIDA, Akihiko KONDO, Takayuki ARAZOE, Shin YOSHIOKA
  • Method for converting nucleic acid sequence of cell specifically converting nucleic acid base of targeted DNA using cell endogenous DNA modifying enzyme, and molecular complex used therein
    Patent number: 11845953
    Abstract: Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.
    Type: Grant
    Filed: March 20, 2018
    Date of Patent: December 19, 2023
    Assignee: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Keiji Nishida, Akihiko Kondo, Takayuki Arazoe, Shin Yoshioka
  • METHOD FOR INCREASING MUTATION INTRODUCTION EFFICIENCY IN GENOME SEQUENCE MODIFICATION TECHNIQUE, AND MOLECULAR COMPLEX TO BE USED THEREFOR
    Publication number: 20200377910
    Abstract: The present invention provides a method of modifying a targeted site of a double-stranded DNA, comprising a step of introducing a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a double-stranded DNA and PmCDA1 are bonded, into a cell containing the double-stranded DNA, and culturing the cell at a low temperature at least temporarily to convert the targeted site, i.e., the target nucleotide sequence and nucleotides in the vicinity thereof, to other nucleotides, or delete the targeted site, or insert nucleotide into the site.
    Type: Application
    Filed: April 21, 2017
    Publication date: December 3, 2020
    Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Keiji NISHIDA, Akihiko KONDO, Takayuki ARAZOE, Zenpei SHIMATANI
  • METHOD FOR CONVERTING NUCLEIC ACID SEQUENCE OF CELL SPECIFICALLY CONVERTING NUCLEIC ACID BASE OF TARGETED DNA USING CELL ENDOGENOUS DNA MODIFYING ENZYME, AND MOLECULAR COMPLEX USED THEREIN
    Publication number: 20200010856
    Abstract: Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.
    Type: Application
    Filed: March 20, 2018
    Publication date: January 9, 2020
    Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Keiji NISHIDA, Akihiko KONDO, Takayuki ARAZOE, Shin YOSHIOKA