Abstract: An image forming system includes an applying unit that applies a crimping toner having pressure-responsiveness onto at least a portion of a surface of a recording medium to form a net-dot structure having 300 or more of screen lines, a heating unit that heats the crimping toner applied to the recording medium, and a pressurizing unit that pressurizes, in a thickness direction, a stacked body in which the recording medium is folded with the heated crimping toner interposed or a stacked body in which the recording medium and another medium are stacked with the heated crimping toner interposed.

Abstract: An image forming system includes an image forming unit that forms an image on a recording medium by using a color toner, an applying unit that applies a crimping toner having pressure-responsiveness onto at least a portion of a surface of the recording medium on which the image is formed, a heating unit that heats the crimping toner applied to the recording medium, and a pressurizing unit that pressurizes, in a thickness direction, a stacked body in which the recording medium is folded with the heated crimping toner interposed or a stacked body in which the recording medium and another medium are stacked with the heated crimping toner interposed, in which a volume average particle diameter of the crimping toner is more than a volume average particle diameter of the color toner.

Abstract: A pressure-bonding device that pressure-bonds pressure-bondable toner surfaces to each other which are surfaces of a printing medium on which pressure-bondable toners are formed includes: a pair of rollers that applies a pressure to pressure-bondable surfaces, on which the pressure-bondable toner surfaces of the printing medium face each other, by sandwiching the printing medium in a nip portion; and a heating control mechanism that heats the nip portion.

Abstract: A plate data forming device includes: a processor configured to: form plate data of a pressure-bondable toner that indicate at least one of an amount of the pressure-bondable toner, which is formed on a printing medium, or a region, in which the pressure-bondable toner is formed on the printing medium, on the basis of a pressure-bondable toner formation condition indicating a condition for forming the pressure-bondable toner on the printing medium.

Abstract: The present disclosure relates to a device including a reagent portion in which a chemiluminescent indicator and a chemiluminescent substrate for the indicator are disposed, and a base on which the reagent portion is formed. The chemiluminescent indicator and the chemiluminescent substrate are disposed independently from each other in the reagent portion in such a manner that the chemiluminescent indicator and the chemiluminescent substrate can react with each other when a sample is supplied to the reagent portion. The present disclosure also relates to a remote diagnosis system including an imaging terminal for detecting a luminescent signal generated when a reagent is supplied to the device and an information processing unit for processing luminescent signal data obtained by the imaging terminal. The imaging terminal and the information processing unit can bi-directionally communicate with each other via a network.

Abstract: Provided is a method of detecting a biological material, by which quantitative measurement can be performed easily. The method of detecting a biological material in a sample includes: mixing, with the sample, a fusion protein (C) in which a protein (A) capable of binding the biological material and a chemiluminescent protein (B) are fused together and a substrate for the chemiluminescent protein (B); and observing a luminescent signal from the sample, wherein the protein (A) and the protein (B) are linked in such a manner that resonance energy transfer can occur, the protein (A) is either a protein (A1) that can emit fluorescence in a state where the biological material is bound thereto or a protein (A2) capable of binding an autofluorescent molecule as the biological material, and the protein (B) can excite fluorescence or autofluorescence of the protein (A) with its luminescence energy.

Abstract: A biological specimen containing a chemiluminescence substance that generates chemiluminescence is observed in a living state under a microscope. The microscope includes a light source that outputs control light that changes the state of the chemiluminescence, a defining unit that defines the radiation pattern of the control light with which an observation surface of the biological specimen is irradiated, and a detector that detects the chemiluminescence from the biological specimen.

Abstract: Provided is a method for causing a subject including a luminescent protein to emit light. The method provided is a method for causing a subject to emit light, wherein the subject is a plant body that has a fusion luminescent protein in a cell wall thereof, or a processed product of the same; the method includes the step of bringing a substrate composition into contact with the fusion luminescent protein; the fusion luminescent protein is a protein that includes a chemiluminescent protein moiety, a fluorescent protein moiety, and a moiety that connects the chemiluminescent protein moiety and the fluorescent protein moiety so that resonance energy transfer can occur from the chemiluminescent protein moiety to the fluorescent protein moiety; and the substrate composition contains a substrate of the chemiluminescent protein.

Abstract: Provided is a green-yellow fluorescent protein with high pH stability (with reduced pH sensitivity). In one or more embodiments, the fluorescent protein is a fluorescent protein comprising an amino acid sequence from position 2 to 225 of the amino acid sequence of SEQ ID NO: 1, at least a mutation being introduced into the sequence, wherein the above-mentioned mutation is selected from the group consisting of F149T or S or A, L158T or S or A, H160T or S or A, Y174T or S or A, and Y192T or S or A as well as combinations of 2 to 5 thereof.

Abstract: Provided are a device with excellent operability and portability and a determination system that uses the device. The present disclosure relates to a device including a reagent portion in which a chemiluminescent indicator and a chemiluminescent substrate for the indicator are disposed, and a base on which the reagent portion is formed. The chemiluminescent indicator and the chemiluminescent substrate are disposed independently from each other in the reagent portion in such a manner that the chemiluminescent indicator and the chemiluminescent substrate can react with each other when a sample is supplied to the reagent portion. The present disclosure also relates to a remote diagnosis system including an imaging terminal for detecting a luminescent signal generated when a reagent is supplied to the device and an information processing unit for processing luminescent signal data obtained by the imaging terminal.

Abstract: Provided is a method of detecting a biological material, by which quantitative measurement can be performed easily. The method of detecting a biological material in a sample includes: mixing, with the sample, a fusion protein (C) in which a protein (A) capable of binding the biological material and a chemiluminescent protein (B) are fused together and a substrate for the chemiluminescent protein (B); and observing a luminescent signal from the sample, wherein the protein (A) and the protein (B) are linked in such a manner that resonance energy transfer can occur, the protein (A) is either a protein (A1) that can emit fluorescence in a state where the biological material is bound thereto or a protein (A2) capable of binding an autofluorescent molecule as the biological material, and the protein (B) can excite fluorescence or autofluorescence of the protein (A) with its luminescence energy.

Abstract: Provided is a green-yellow fluorescent protein with high pH stability (with reduced pH sensitivity). In one or more embodiments, the fluorescent protein is a fluorescent protein comprising an amino acid sequence from position 2 to 225 of the amino acid sequence of SEQ ID NO: 1, at least a mutation being introduced into the sequence, wherein the above-mentioned mutation is selected from the group consisting of F149T or S or A, L158T or S or A, H160T or S or A, Y174T or S or A, and Y192T or S or A as well as combinations of 2 to 5 thereof.

Abstract: A biological specimen containing a chemiluminescence substance that generates chemiluminescence is observed in a living state under a microscope. The microscope includes a light source that outputs control light that changes the state of the chemiluminescence, a defining unit that defines the radiation pattern of the control light with which an observation surface of the biological specimen is irradiated, and a detector that detects the chemiluminescence from the biological specimen.

Abstract: A photo-switching fluorescent protein of a type in which wavelengths for switching fluorescence on and off and wavelength for fluorescence excitation are all independent of one another is provided, which has an amino acid sequence in which at least an S208G mutation is introduced into SEQ ID NO: 1. The fluorescent protein may have an amino acid sequence in which at least three mutations, I47V, M153T, and S208G, are introduced into SEQ ID NO: 1. The fluorescent protein may have an amino acid sequence in which at least five mutations, I47V, T59S, M153T, S208G, and M233T, are introduced into SEQ ID NO: 1. The fluorescent protein may have an amino acid sequence in which at least six mutations, I47V, T59S, M69Q, M153T, S208G, and M233T, are introduced into SEQ ID NO: 1.

Abstract: Provided is a fluorescent protein that has a fast photo-switching speed and a high photostability and that switches from a non-fluorescent state to a fluorescent state by being irradiated with light for fluorescence excitation and switches from a fluorescent state to a non-fluorescent state by being irradiated with light having a specific wavelength that does not cause fluorescence excitation. The present invention relates to (a) A protein having an amino acid sequence of Sequence ID No. 1. (b) A protein that has an amino acid sequence of Sequence ID No. 1 in which one to several amino acids are deleted, substituted, and/or added.

Abstract: Provided is an optical microscope capable of maintaining an objective lens in a focus state without adversely affecting a captured image even when a substance with a weak emission is used as an observation target object. The optical microscope includes an observation optical system capable of capturing, with an image pickup device (10), an optical image of an observation target object (1) that has been obtained with an objective lens (5), and sending out the optical image as an image signal; and an autofocus device that adjusts a focal position of the objective lens based on autofocus light emitted from a focusing light source (11) so as to focus on the observation target object, wherein the autofocus light is emitted from the focusing light source during a non-capturing period during which the image pickup device is not capturing an image of the observation target object.

Abstract: Provided is a fluorescent protein that has a fast photo-switching speed and a high photostability and that switches from a non-fluorescent state to a fluorescent state by being irradiated with light for fluorescence excitation and switches from a fluorescent state to a non-fluorescent state by being irradiated with light having a specific wavelength that does not cause fluorescence excitation. The present invention relates to (a) A protein having an amino acid sequence of Sequence ID No. 1. (b) A protein that has an amino acid sequence of Sequence ID No. 1 in which one to several amino acids are deleted, substituted, and/or added.

Abstract: A fluorescence observation method of the present invention for detecting plural types of fluorescence emitted from two or more kinds of fluorescent molecules includes: subjecting each of the two or more kinds of fluorescent molecules to multi-photon excitation by exciting light having an exciting wavelength equal to or shorter than 700 nm in a visible region, to generate fluorescence upon making use of an absorption wavelength band in a deep ultraviolet region of each of the two or more kinds of fluorescent molecules; and simultaneously detecting plural types of fluorescence generated on a shorter-wavelength side or on both of the shorter-wavelength side and a longer-wavelength side of the exciting wavelength of the exciting light.

Abstract: Provided is an optical microscope capable of maintaining an objective lens in a focus state without adversely affecting a captured image even when a substance with a weak emission is used as an observation target object. The optical microscope includes an observation optical system capable of capturing, with an image pickup device (10), an optical image of an observation target object (1) that has been obtained with an objective lens (5), and sending out the optical image as an image signal; and an autofocus device that adjusts a focal position of the objective lens based on autofocus light emitted from a focusing light source (11) so as to focus on the observation target object, wherein the autofocus light is emitted from the focusing light source during a non-capturing period during which the image pickup device is not capturing an image of the observation target object.

Abstract: A fluorescence observation method of the present invention for detecting plural types of fluorescence emitted from two or more kinds of fluorescent molecules includes: subjecting each of the two or more kinds of fluorescent molecules to multi-photon excitation by exciting light having an exciting wavelength equal to or shorter than 700 nm in a visible region, to generate fluorescence upon making use of an absorption wavelength band in a deep ultraviolet region of each of the two or more kinds of fluorescent molecules; and simultaneously detecting plural types of fluorescence generated on a shorter-wavelength side or on both of the shorter-wavelength side and a longer-wavelength side of the exciting wavelength of the exciting light.