Abstract: Using a reversed-phase high-performance liquid chromatographic (HPLC) technique, a mixture of antimycins A was separated into eight hitherto unreported subcomponents, Ala, Alb, A2a, A2b, A3a, A3b, Ma, and A4b. Although a base-line resolution of the known four major antimycins Al, A2, A3, and A4 was readily achieved with mobile phases containing acetate buffers, the separation of the new antibiotic subcomponents was highly sensitive to variation in mobile phase conditions. The type and composition of organic modifiers, the nature of buffer salts, and the concentration of added electrolytes had profound effects on capacity factors, separation factors, and peak resolution values. Of the numerous chromatographic systems examined, a mobile phase consisting of methanol-water (70:30) and 0.005 M tetrabutylammonium phosphate at pH 3.0 yielded the most satisfactory results for the separation of the subcomponents.

Abstract: Provided is a method of producing an osteoblast construct from iPS cells, the method including the steps of: (1) inducing formation of an embryoid body by subjecting undifferentiated iPS cells to non-adherent culture; (2) inducing differentiation of the iPS cells into mesodermal cells by subjecting the embryoid body of the iPS cells obtained in the step (1) to non-adherent culture; and (3) inducing differentiation into osteoblasts by subjecting the mesodermal cells of the iPS cells obtained in the step (2) to non-adherent culture, wherein the steps (1) and (2) are each performed using a culture vessel comprising a bottom surface and a circular side wall arranged upright on the bottom surface, the bottom surface having a plurality of depressed portions arranged independently of each other.

Abstract: In a bending tube for endoscope on which torsion preventing tab mechanisms are provided on the respective middle parts of first and second slots for bending located in an area from the proximal end of a bending tube body to a predetermined position on the distal end side (the torsion suppressing area), and the bending tube body being permitted to bend by the first and second slots for bending, the torsion preventing tab mechanism of at least any one of the first and second slots for bending arranged on the proximal end side of each of arrays (first and second arrays), other than the first and second slots for bending located at the most proximal end, among the first and second slots for bending arrayed in a same line, respectively, along a longitudinal direction of the bending tube body, is removed.

Abstract: In a bending tube for endoscope on which torsion preventing tab mechanisms are provided on the respective middle parts of first and second slots for bending located in an area from the proximal end of a bending tube body to a predetermined position on the distal end side (the torsion suppressing area), and the bending tube body being permitted to bend by the first and second slots for bending, the torsion preventing tab mechanism of at least any one of the first and second slots for bending arranged on the proximal end side of each of arrays (first and second arrays), other than the first and second slots for bending located at the most proximal end, among the first and second slots for bending arrayed in a same line, respectively, along a longitudinal direction of the bending tube body, is removed.