Patents by Inventor Takeshi Ohura
Takeshi Ohura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10222348Abstract: The biological polymer analyzing equipment with nanopore includes a chamber part having a chamber having a sample introduction section and a sample outflow section separated by a substrate; a first electrode provided in the sample introduction section and a second electrode provided in the sample outflow section; a thin membrane formed on the substrate; a nanopore provided in the thin membrane of the substrate and communicating between the sample introduction section and the sample outflow section; a third electrode provided near the nanopore of the substrate; and a voltage applying member to electrodes, wherein the voltage applying member includes a member for applying voltages between the first electrode and the third electrode, between the first electrode and the second electrode, respectively, and between the third electrode and the second electrode, and relates to a method for analyzing a biological polymer using the biological polymer analyzing equipment with nanopore.Type: GrantFiled: July 25, 2012Date of Patent: March 5, 2019Assignee: Hitachi High-Technologies CorporationInventor: Takeshi Ohura
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Patent number: 9562809Abstract: The present invention provides a device and method for analyzing the characteristics of a biopolymer with excellent mechanical stability, high spatial resolution and sensitivity using a simple device construction. Specifically, the Raman scattered light of a biopolymer is measured and the properties of monomer units forming the biopolymer are analyzed by using a biopolymer property analysis chip (100a) characterized by comprising: a solid substrate (110); at least one nanopore (120) disposed in the solid substrate (110); and one or more electrically conductive thin films (130a, 130b) disposed on the solid substrate (110). The biopolymer property analysis chip (100a) is characterized in that the electrically conductive thin films (130a, 130b) are disposed partially on the solid substrate (110) where the nanopore (120) is formed and a biopolymer which has penetrated into the nanopore (120) is caused to generate Raman scattered light by means of irradiation with external light.Type: GrantFiled: July 21, 2011Date of Patent: February 7, 2017Assignee: Hitachi High-Technologies CorporationInventors: Satoshi Ozawa, Takashi Anazawa, Rena Akahori, Satoshi Takahashi, Takeshi Ohura, Masashi Kiguchi
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Publication number: 20140243214Abstract: In an FET configuration having a channel with a small thickness, transistor characteristics vary for different FETs in the same array, and therefore when the same gate voltage is applied, the sensitivities of DNA detection may be insufficient. To this end, the change in the channel current when DNA passes through the nanopore is detected while applying an optimum gate voltage for each nanopore FET to attain a predetermined channel current value to a plurality of nanopore FETs disposed on the same substrate, and four types of bases constituting DNA are distinguished.Type: ApplicationFiled: February 26, 2013Publication date: August 28, 2014Applicant: Hitachi, Ltd.Inventors: Takanobu Haga, Itaru Yanagi, Naoshi Itabashi, Yoshimitsu Yanagawa, Takeshi Ohura, Takashi Anazawa
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Publication number: 20140158540Abstract: The biological polymer analyzing equipment with nanopore includes a chamber part having a chamber having a sample introduction section and a sample outflow section separated by a substrate; a first electrode provided in the sample introduction section and a second electrode provided in the sample outflow section; a thin membrane formed on the substrate; a nanopore provided in the thin membrane of the substrate and communicating between the sample introduction section and the sample outflow section; a third electrode provided near the nanopore of the substrate; and a voltage applying member to electrodes, wherein the voltage applying member includes a member for applying voltages between the first electrode and the third electrode, between the first electrode and the second electrode, respectively, and between the third electrode and the second electrode, and relates to a method for analyzing a biological polymer using the biological polymer analyzing equipment with nanopore.Type: ApplicationFiled: July 25, 2012Publication date: June 12, 2014Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATIONInventor: Takeshi Ohura
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Patent number: 8702949Abstract: The solution reservoir apparatus of the capillary electrophoresis apparatus securely affixes an evaporation-preventing membrane to a container when a capillary is inserted or withdrawn, without extending the cathode end of the capillary. The solution reservoir apparatus comprises a container for reserving a sample or solution, a cover having a bore through which the capillary is passed and covering the container, an evaporation-preventing membrane having a capillary hole through which the capillary is passed, and a container holder for holding the container. The evaporation-preventing membrane has a projection provided at the periphery of the capillary hole, the projection of the evaporation-preventing membrane is engaged with the bore on the cover when the evaporation-preventing membrane is positioned on the cover, and the evaporation-preventing membrane is supported by the cover.Type: GrantFiled: April 8, 2010Date of Patent: April 22, 2014Assignee: Hitachi High-Technologies CorporationInventors: Takeshi Ohura, Ryoji Inaba, Manabu Akiba, Takashi Gomi, Mari Kotoura
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Patent number: 8685220Abstract: The invention provides a capillary electrophoresis apparatus which can improve the operability and measuring speed. According to the invention, a sensor for identifying the type of sample containers is fixed at the position away from a capillary anode electrode. The sensor is made to be closer to the sample containers by moving a moving stage so that the sample containers disposed on the moving stage can be identified by the sensor. A fixing apparatus for fixing at least a pair of sample containers is provided on the moving stage.Type: GrantFiled: July 26, 2012Date of Patent: April 1, 2014Assignee: Hitachi High-Technologies CorporationInventors: Takeshi Ohura, Ryoji Inaba, Yoshitaka Kodama, Hiromi Yamashita, Manabu Akiba, Tomohiro Shoji
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Publication number: 20130176563Abstract: The present invention provides a device and method for analyzing the characteristics of a biopolymer with excellent mechanical stability, high spatial resolution and sensitivity using a simple device construction. Specifically, the Raman scattered light of a biopolymer is measured and the properties of monomer units forming the biopolymer are analyzed by using a biopolymer property analysis chip (100a) characterized by comprising: a solid substrate (110); at least one nanopore (120) disposed in the solid substrate (110); and one or more electrically conductive thin films (130a, 130b) disposed on the solid substrate (110). The biopolymer property analysis chip (100a) is characterized in that the electrically conductive thin films (130a, 130b) are disposed partially on the solid substrate (110) where the nanopore (120) is formed and a biopolymer which has penetrated into the nanopore (120) is caused to generate Raman scattered light by means of irradiation with external light.Type: ApplicationFiled: July 21, 2011Publication date: July 11, 2013Inventors: Satoshi Ozawa, Takashi Anazawa, Rena Akahori, Satoshi Takahashi, Takeshi Ohura, Masashi Kiguchi
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Patent number: 8349161Abstract: Bubbles can be removed regardless of an individual difference of a pump to fill an electrophoresis medium into a capillary. Of flow passages formed between an inner side surface of a container for accommodating the electrophoresis medium and a side surface of a plunger, one of the flow passages causing an electrophoresis medium to be easily stagnant is formed to have the cross-sectional area larger than the cross-sectional area of the other flow passage on the opposite side. In other words, the flow passage portion causing the electrophoresis medium to be easily stagnant is formed in such a manner as to increase a flow amount of the electrophoresis medium. This can eliminate a region having an extremely small amount of electrophoresis medium flow in the pump.Type: GrantFiled: April 6, 2010Date of Patent: January 8, 2013Assignee: Hitachi High-Technologies CorporationInventors: Takashi Gomi, Ryoji Inaba, Taro Nakazawa, Takeshi Ohura, Mari Kotoura
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Publication number: 20120292189Abstract: The invention provides a capillary electrophoresis apparatus which can improve the operability and measuring speed. According to the invention, a sensor for identifying the type of sample containers is fixed at the position away from a capillary anode electrode. The sensor is made to be closer to the sample containers by moving a moving stage so that the sample containers disposed on the moving stage can be identified by the sensor. A fixing apparatus for fixing at least a pair of sample containers is provided on the moving stage.Type: ApplicationFiled: July 26, 2012Publication date: November 22, 2012Applicant: Hitachi High-Technologies CorporationInventors: Takeshi OHURA, Ryoji Inaba, Yoshitaka Kodama, Hiromi Yamashita, Manabu Akiba, Tomohiro Shoji
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Patent number: 8262888Abstract: There is provided a capillary electrophoresis apparatus wherein coupling efficiency of exciting irradiation light to a capillary array does not decline even if any one capillary array of two capillary arrays is removed. Light emission generated by capillaries of each capillary array of 2n (n is a positive integer) capillary arrays radiating exciting light from one side is detected by one optical detection system.Type: GrantFiled: March 4, 2008Date of Patent: September 11, 2012Assignee: Hitachi High-Technologies CorporationInventors: Ryoji Inaba, Motohiro Yamazaki, Tomohiro Shoji, Takeshi Ohura, Takashi Gomi
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Patent number: 8252162Abstract: The invention provides a capillary electrophoresis apparatus which can improve the operability and measuring speed. According to the invention, a sensor for identifying the type of sample containers is fixed at the position away from a capillary anode electrode. The sensor is made to be closer to the sample containers by moving a moving stage so that the sample containers disposed on the moving stage can be identified by the sensor. A fixing apparatus for fixing at least a pair of sample containers is provided on the moving stage.Type: GrantFiled: December 24, 2008Date of Patent: August 28, 2012Assignee: Hitachi High-Technologies CorporationInventors: Takeshi Ohura, Ryoji Inaba, Yoshitaka Kodama, Hiromi Yamashita, Manabu Akiba, Tomohiro Shoji
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Publication number: 20120043211Abstract: Provided is a capillary electrophoresis apparatus capable of avoiding electric discharge even when a conductive component is arranged near a cathode end of a capillary. The capillary electrophoresis apparatus of the present invention has a structure in which a spatial distance and a creeping distance between an electrode attached to the cathode end of the capillary and the conductive component are large even when the conductive component is arranged near the cathode end of the capillary. That is to say, the capillary electrode, which protrudes from a lower surface of the load header, penetrates through a space between the load header and an anti-evaporation film and further penetrates through a capillary hole formed on the anti-evaporation film to extend into a container. At least a portion exposed to the space between the load header and the anti-evaporation film of the capillary electrode is covered with an insulating member.Type: ApplicationFiled: April 8, 2010Publication date: February 23, 2012Inventors: Yasutaka Otsuka, Toshiyuki Sakurai, Tomohiro Shoji, Takeshi Ohura
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Publication number: 20120031762Abstract: Bubbles can be removed regardless of an individual difference of a pump to fill an electrophoresis medium into a capillary. Of flow passages formed between an inner side surface of a container for accommodating the electrophoresis medium and a side surface of a plunger, one of the flow passages causing an electrophoresis medium to be easily stagnant is formed to have the cross-sectional area larger than the cross-sectional area of the other flow passage on the opposite side. In other words, the flow passage portion causing the electrophoresis medium to be easily stagnant is formed in such a manner as to increase a flow amount of the electrophoresis medium. This can eliminate a region having an extremely small amount of electrophoresis medium flow in the pump.Type: ApplicationFiled: April 6, 2010Publication date: February 9, 2012Inventors: Takashi Gomi, Ryoji Inaba, Taro Nakazawa, Takeshi Ohura, Mari Kotoura
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Publication number: 20120024707Abstract: The solution reservoir apparatus of the capillary electrophoresis apparatus securely affixes an evaporation-preventing membrane to a container when a capillary is inserted or withdrawn, without extending the cathode end of the capillary. The solution reservoir apparatus comprises a container for reserving a sample or solution, a cover having a bore through which the capillary is passed and covering the container, an evaporation-preventing membrane having a capillary hole through which the capillary is passed, and a container holder for holding the container. The evaporation-preventing membrane has a projection provided at the periphery of the capillary hole, the projection of the evaporation-preventing membrane is engaged with the bore on the cover when the evaporation-preventing membrane is positioned on the cover, and the evaporation-preventing membrane is supported by the cover.Type: ApplicationFiled: April 8, 2010Publication date: February 2, 2012Inventors: Takeshi Ohura, Ryoji Inaba, Manabu Akiba, Takashi Gomi, Mari Kotoura
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Patent number: 7981267Abstract: An object of the present invention is to prevent a variation in heat dissipating effect of a capillary between a holder part and an oven, to improve reproducibility of migration time, and to reduce a variation of migration time among capillaries in a single electrophoresis run. A cylindrical wall is formed in an upper part of a septa that covers a container holding a solution, and the cylindrical wall surrounds a capillary hole through which a capillary penetrates. Accordingly, the capillary is prevented from being directly affected by wind generated between the septa and the oven.Type: GrantFiled: January 13, 2009Date of Patent: July 19, 2011Assignee: Hitachi High-Technologies CorporationInventors: Toshiyuki Sakurai, Takeshi Ohura
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Patent number: 7943027Abstract: The object of the present invention is that a dynamic range is extended in an electrophoresis unit and concentration differences among a plurality of samples measured simultaneously are increased. An irradiation time to the samples is adjusted during analysis without changing a sampling time. By shortening the irradiation time, a fluorescence amount of the samples is reduced to cause signal intensity detected by a detector to physically decrease. If the irradiation time is very short (several 100 msec), the irradiation time and fluorescence intensity are in a direct proportional relationship. It is known that, if the irradiation time is reduced to 1/n, the fluorescence intensity, that is, signal intensity to be detected will be 1/n. Thus, for data whose irradiation time is reduced to 1/n during analysis, data obtained by multiplying a substantially measured value by n is used for data analysis as a true value to be originally acquired.Type: GrantFiled: November 6, 2007Date of Patent: May 17, 2011Assignee: Hitachi High-Technologies CorporationInventors: Motohiro Yamazaki, Ryoji Inaba, Satoshi Takahashi, Tomohiro Shoji, Takeshi Ohura, Takashi Gomi
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Publication number: 20090211911Abstract: The invention provides a capillary electrophoresis apparatus which can improve the operability and measuring speed. According to the invention, a sensor for identifying the type of sample containers is fixed at the position away from a capillary anode electrode. The sensor is made to be closer to the sample containers by moving a moving stage so that the sample containers disposed on the moving stage can be identified by the sensor. A fixing apparatus for fixing at least a pair of sample containers is provided on the moving stage.Type: ApplicationFiled: December 24, 2008Publication date: August 27, 2009Inventors: Takeshi Ohura, Ryoji Inaba, Yoshitaka Kodama, Hiromi Yamashita, Manabu Akiba, Tomohiro Shoji
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Publication number: 20090200169Abstract: An object of the present invention is to prevent a variation in heat dissipating effect of a capillary between a holder part and an oven, to improve reproducibility of migration time, and to reduce a variation of migration time among capillaries in a single electrophoresis run. A cylindrical wall is formed in an upper part of a septa that covers a container holding a solution, and the cylindrical wall surrounds a capillary hole through which a capillary penetrates. Accordingly, the capillary is prevented from being directly affected by wind generated between the septa and the oven.Type: ApplicationFiled: January 13, 2009Publication date: August 13, 2009Inventors: Toshiyuki Sakurai, Takeshi Ohura
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Publication number: 20080245716Abstract: A monolithic separation column and a liquid chromatography system using the same are provided by which the pressure tightness is improved, and the manufacturing is enabled at a low temperature, thus preventing the degradation of the separation performance. A monolithic rod 118 is made of a porous body and has a cylindrical shape. A coat material 116 coats an outer circumference of the monolithic rod 118 and includes an uneven portion 116R on an outer face thereof A holder 112, into which the monolithic rod 118 coated with the coat material 116 is inserted, includes a taper portion 112T on an inner face thereof. A potting media 114 is fitted or filled between the coat material 116 and the holder 112.Type: ApplicationFiled: March 21, 2008Publication date: October 9, 2008Inventors: Takeshi Ohura, Tomohiro Shoji, Daizo Tokinaga, Tomonari Morioka, Yoshihiro Nagaoka, Yoshitaka Kodama
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Publication number: 20080217177Abstract: There is provided a capillary electrophoresis apparatus wherein coupling efficiency of exciting irradiation light to a capillary array does not decline even if any one capillary array of two capillary arrays is removed. Light emission generated by capillaries of each capillary array of 2n (n is a positive integer) capillary arrays radiating exciting light from one side is detected by one optical detection system.Type: ApplicationFiled: March 4, 2008Publication date: September 11, 2008Inventors: Ryoji Inaba, Motohiro Yamazaki, Tomohiro Shoji, Takeshi Ohura, Takashi Gomi