Abstract: A means of simultaneously detecting the characteristics of a plurality of proteins is provided. The protein detection method is a method of simultaneously detecting a plurality of proteins contained in a sample and comprises the steps of: arranging, on a substrate, a plurality of substances to be arranged, which form specific complexes with analytes; forming a plurality of first specific conjugates by allowing a specific binding reaction to occur on the above substrate between the analytes contained in the sample and the above plurality of arranged substances; forming a plurality of second specific complexes by allowing a specific binding reaction to occur between substances, which form specific complexes with the analytes and are labeled with a fluorescence reagent or an RI reagent, and the above plurality of first specific conjugates; and identifying the analytes contained in the sample by detecting the labels contained in the above plurality of second specific complexes.