Abstract: The present invention relates to a Thermus aquaticus (Taq) polymerase having a strand displacement activity in which an amino acid residue in a template DNA binding site of the DNA polymerase is substituted with an amino acid to increase a total charge in the site, a nucleic acid encoding the polymerase, a vector containing the nucleic acid, a transformant containing the vector containing the nucleic acid or the nucleic acid, a method for producing the polymerase, a method for amplifying nucleic acids utilizing the polymerase, and a kit containing the polymerase. According to the present invention, a DNA polymerase having a high thermostability, capable of efficiently replicating a long-strand of a template DNA, and having a strong strand displacement activity is provided.

Abstract: A polypeptide having a mismatch endonuclease activity of recognizing a mismatch and cleaving the mismatch; a mismatch-specific cleavage reaction using the polypeptide; a method for removing an error in a nucleic acid amplification reaction utilizing the polypeptide; a method for inhibiting the amplification of a nucleic acid comprising a specific nucleotide sequence during a nucleic acid amplification reaction; and a method for detecting a nucleic acid having a single-nucleotide polymorphism mutation utilizing the inhibition method.

Abstract: Provided are various novel DNA polymerases. Provided are: a DNA polymerase comprising: an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 12, which has a substitution of arginine at position 651 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid; and a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 14, which has a substitution of proline at position 653 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid.

Abstract: The present invention relates to a fusion polypeptide containing, in a direction of from an N-terminal side to a C-terminal side, one or more peptides which bind to a PCNA, and a polypeptide having a DNA polymerase activity; a method for amplifying nucleic acids using the polypeptide; and a composition and a kit, containing the polypeptide. According to the present invention, it is made possible to amplify a long-strand DNA in a short time in amplifying nucleic acids in the presence of PCNA even with a Pol I-type DNA polymerase.

Abstract: Provided are various novel DNA polymerases. Provided are: a DNA polymerase comprising: an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 12, which has a substitution of arginine at position 651 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid; and a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 14, which has a substitution of proline at position 653 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid.

Abstract: The present invention relates to a Thermus aquaticus (Taq) polymerase having a strand displacement activity in which an amino acid residue in a template DNA binding site of the DNA polymerase is substituted with an amino acid to increase a total charge in the site, a nucleic acid encoding the polymerase, a vector containing the nucleic acid, a transformant containing the vector containing the nucleic acid or the nucleic acid, a method for producing the polymerase, a method for amplifying nucleic acids utilizing the polymerase, and a kit containing the polymerase. According to the present invention, a DNA polymerase having a high thermostability, capable of efficiently replicating a long-strand of a template DNA, and having a strong strand displacement activity is provided.

Abstract: A polypeptide having a mismatch endonuclease activity of recognizing a mismatch and cleaving the mismatch; a mismatch-specific cleavage reaction using the polypeptide; a method for removing an error in a nucleic acid amplification reaction utilizing the polypeptide; a method for inhibiting the amplification of a nucleic acid comprising a specific nucleotide sequence during a nucleic acid amplification reaction; and a method for detecting a nucleic acid having a single-nucleotide polymorphism mutation utilizing the inhibition method.

Abstract: Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A737-A738-A739-A740-A741-A742-A743-A744-A745-” between the amino acid residue at position 736 and the amino acid residue at position 737, wherein: A737 is an amino acid residue having a non-polar aliphatic side chain; A738 is an amino acid residue having a non-polar aliphatic side chain; A739 is an amino acid residue having a positively charged side chain; A740 is an amino acid residue having a positively charged side chain; A741 is an amino acid residue having a non-polar aliphatic side chain; A742 is an amino acid residue having a non-polar aliphatic side chain; A743 is any given amino acid residue; A744 is an amino acid residue having a positively charged side chain; and A745 is an amino acid residue having a non-polar aliphatic side chain).

Abstract: A thermoelectric conversion module includes: a piping for flowing a compressible fluid; high temperature electrodes which are provided on a top surface and a bottom surface of the piping and electrically insulated from the piping; thermoelectric conversion elements which are provided on the respective high temperature electrodes, each element containing at least a pair of p-type thermoelectric semiconductor and n-type thermoelectric semiconductor which are electrically connected in series with one another; low temperature electrodes which are provided on the respective thermoelectric conversion elements and electrically connect the p-type thermoelectric semiconductor in series with the n-type thermoelectric semiconductor; and a first case member for accommodating the piping, the high temperature electrodes, the thermoelectric conversion elements and the low temperature electrodes so as to form a space for flowing a refrigerant for the low temperature electrodes.

Abstract: In an airtight container in which the flow tube is arranged inside of the housing, the housing includes the movable plate part in which the deformation part having flexibility is arranged around the inner rigid part, and the thermoelectric conversion module is sandwiched between the inner solid part and inner plate part of the flow tube in the airtight container. By reducing pressure in the airtight container, the deformation part of the movable plate part deforms and the inner plate part contacts the thermoelectric conversion module in a uniformly pressed condition. The inner solid part of the movable plate part is cooled by the cooling part and the inner plate part is heated by supplying the heating fluid in the flow tube, so that the temperature difference occurs in the thermoelectric conversion module, thereby generating electricity.

Abstract: Provided are various novel DNA polymerases. Provided are: a DNA polymerase comprising: an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 12, which has a substitution of arginine at position 651 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid; and a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 14, which has a substitution of proline at position 653 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid.

Abstract: Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A737-A738-A739-A740-A741-A742-A743-A744-A745-” between the amino acid residue at position 736 and the amino acid residue at position 737, wherein: A737 is an amino acid residue having a non-polar aliphatic side chain; A738 is an amino acid residue having a non-polar aliphatic side chain; A739 is an amino acid residue having a positively charged side chain; A740 is an amino acid residue having a positively charged side chain; A741 is an amino acid residue having a non-polar aliphatic side chain; A742 is an amino acid residue having a non-polar aliphatic side chain; A743 is any given amino acid residue; A744 is an amino acid residue having a positively charged side chain; and A745 is an amino acid residue having a non-polar aliphatic side chain).

Abstract: To produce a temperature difference in the thermoelectric conversion module, a tabular member of the cooling side arranged side of the thermoelectric conversion module is fitted in a uniform pressed condition on the thermoelectric conversion module. In the airtight container in which the flow tube penetrates the housing and the thermoelectric conversion module is arranged in the reduced pressure space between the housing and the flow tube, a tabular member of the cooling side of the housing corresponding to the thermoelectric conversion module is formed by the thin plate that is flexible, and the thermoelectric conversion module is sandwiched between the thin plate and the flow tube. The thin plate contacts the thermoelectric conversion module in a pressed condition by reducing pressure in the reduced pressure space, and the thin plate deforms by following the shape of the thermoelectric conversion module and fits due to its flexibility.

Abstract: A fuel modification apparatus is equipped with evaporators for evaporating a raw fuel as a fuel to be modified, a super heater for elevating the temperature of the above evaporated raw fuel to a temperature required for the modification, a modification vessel for modifying the above raw fuel having an elevated temperature to form a modified gas, and a preheater for preheating a heating fluid for evaporating the above raw fuel in the evaporator. The evaporators are arranged on a concentric circle, surrounding the superheater.

Abstract: The control system includes a welding robot which moves a welding torch along a work for welding the work. A robot controller controls driving of the welding robot. A laser sensor head detects a shape change in the work during welding by the welding torch, and a personal computer obtains instruction information for the welding robot in accordance with the shape change, based on a result of detection by the laser sensor head. The robot controller moves the welding torch by controlling the driving of the working robot, based on the instruction information obtained by the personal computer.

Abstract: The present invention provides a three-piece rectangular can which can overcome drawbacks (joint defect) of a can body joint portion of a three-piece can, and is of a new type which overcomes shortage of a can body strength which a two-piece can possesses, and exhibits excellent liquid leakage resistance, excellent can body strength and excellent heat radiation property or the like even when the can is used as a casing of a battery or electric equipment.

Abstract: A fuel modification apparatus is equipped with evaporators for evaporating a raw fuel as a fuel to be modified, a super heater for elevating the temperature of the above evaporated raw fuel to a temperature required for the modification, a modification vessel for modifying the above raw fuel having an elevated temperature to form a modified gas, and a pre-heater for preheating a heating fluid for evaporating the above raw fuel in the evaporator. The evaporators are arranged on a concentric circle, surrounding the superheater.

Abstract: A heat exchanger includes: a casing; an external cylinder which is arranged inside the casing with a clearance formed between the external cylinder and an inner surface of the casing and is supported in a cantilevered manner, has a closed end portion, and forms a first fluid passage between the external cylinder and the casing; and an internal cylinder which is arranged inside the external cylinder with a clearance formed between the internal cylinder and an inner surface of the external cylinder and is supported in a cantilevered manner, is open at both ends, and forms a second fluid passage, which is turned around at the closed end portion of the external cylinder, between the internal cylinder and the external cylinder.

Abstract: The control system includes a welding robot (10) which moves a welding torch (16) along a work (W) for welding the work (W), a robot controller (40) for controlling driving of the welding robot (10), a laser sensor head (17) for detecting a shape change in the work (W) during welding by the welding torch (16), and a personal computer (30) for obtaining instruction information for the welding robot (10) in accordance with the shape change, based on a result of detection by the laser sensor head (17). The robot controller (40) moves the welding torch (16) by controlling the driving of the working robot, based on the instruction information obtained by the personal computer (30).

Abstract: A heat exchanger includes: a casing; an external cylinder which is arranged inside the casing with a clearance formed between the external cylinder and an inner surface of the casing and is supported in a cantilevered manner, has a closed end portion, and forms a first fluid passage between the external cylinder and the casing; and an internal cylinder which is arranged inside the external cylinder with a clearance formed between the internal cylinder and an inner surface of the external cylinder and is supported in a cantilevered manner, is open at both ends, and forms a second fluid passage, which is turned around at the closed end portion of the external cylinder, between the internal cylinder and the external cylinder.