Patents by Inventor Taku Demura
Taku Demura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11345885Abstract: This invention relates to an agent for inducing a callus comprising a compound represented by Formula (I) or a hydrolysis product of an amide bond thereof: wherein Ar1 represents phenyl substituted with substituent or substituents selected from alkoxy and methylenedioxy; Ar2 represents phenyl substituted with halogen; R1 and R2 each represent hydrogen, alkyl, cyano, or carboxyl; R1 and R2 may together form oxo; R3 to R10 each represent hydrogen or methyl; and R3 and R4, R5 and R6, R7 and R8, and/or R9 and R10 may together form oxo; a method for inducing a callus and a method for plant transformation using such agent for inducing a callus.Type: GrantFiled: April 28, 2020Date of Patent: May 31, 2022Assignee: RIKENInventors: Takeshi Nakano, Tadao Asami, Ayumi Yamagami, Hiroyuki Osada, Misato Ohtani, Taku Demura
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Patent number: 11279941Abstract: It is an object of the present invention to provide a method for introducing a protein into a plant, which is simple and extensively applicable to various types of plant cells and proteins. The above object is achieved by the present invention to provide a complex comprising a protein of interest to be introduced into a target plant cell and a carrier peptide, a method for introducing a protein of interest into a target plant cell using the complex, and a kit comprising a protein of interest to be introduced into a target plant cell and a carrier peptide.Type: GrantFiled: January 19, 2017Date of Patent: March 22, 2022Assignee: RIKENInventors: Keiji Numata, Yoko Horii, Takeshi Yoshizumi, Taku Demura, Yutaka Kodama, Takashi Yamamoto, Tetsushi Sakuma, Kenji Miura, Hiroshi Ezura
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Publication number: 20200318125Abstract: It is an object of the present invention to provide a method for introducing a protein into a plant, which is simple and extensively applicable to various types of plant cells and proteins. The above object is achieved by the present invention to provide a complex comprising a protein of interest to be introduced into a target plant cell and a carrier peptide, a method for introducing a protein of interest into a target plant cell using the complex, and a kit comprising a protein of interest to be introduced into a target plant cell and a carrier peptide.Type: ApplicationFiled: January 19, 2017Publication date: October 8, 2020Applicant: RIKENInventors: Keiji NUMATA, Yoko HORII, Takeshi YOSHIZUMI, Taku DEMURA, Yutaka KODAMA, Takashi YAMAMOTO, Tetsushi SAKUMA, Kenji MIURA, Hiroshi EZURA
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Publication number: 20200255797Abstract: This invention relates to an agent for inducing a callus comprising a compound represented by Formula (I) or a hydrolysis product of an amide bond thereof: wherein Ar1 represents phenyl substituted with substituent or substituents selected from alkoxy and methylenedioxy; Ar2 represents phenyl substituted with halogen; R1 and R2 each represent hydrogen, alkyl, cyano, or carboxyl; R1 and R2 may together form oxo; R3 to R10 each represent hydrogen or methyl; and R3 and R4, R5 and R6, R7 and R8, and/or R9 and R10 may together form oxo; a method for inducing a callus and a method for plant transformation using such agent for inducing a callus.Type: ApplicationFiled: April 28, 2020Publication date: August 13, 2020Applicant: RIKENInventors: Takeshi Nakano, Tadao ASAMI, Ayumi YAMAGAMI, Hiroyuki OSADA, Misato OHTANI, Taku DEMURA
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Patent number: 10723806Abstract: The object of the present invention is to provide a method of introducing a nucleic acid into plant cells, which is simple and widely applicable to various types of plant cells and nucleic acids. The present invention relates to a method of introducing a nucleic acid into a target plant cell, comprising a step of forming a complex by bringing a carrier peptide, wherein the carrier peptide comprises a cell-penetrating sequence and a polycationic sequence, into contact with a nucleic acid and a step of bringing the obtained complex into contact with a target plant cell.Type: GrantFiled: February 27, 2013Date of Patent: July 28, 2020Assignee: RikenInventors: Keiji Numata, Takeshi Yoshizumi, Yutaka Kodama, Misato Ohtani, Taku Demura
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Patent number: 10676711Abstract: This invention relates to an agent for inducing a callus comprising a compound represented by Formula (I) or a hydrolysis product of an amide bond thereof: wherein Ar1 represents phenyl substituted with substituent or substituents selected from alkoxy and methylenedioxy; Ar2 represents phenyl substituted with halogen; R1 and R2 each represent hydrogen, alkyl, cyano, or carboxyl; R1 and R2 may together form oxo; R3 to R10 each represent hydrogen or methyl; and R3 and R4, R5 and R6, R7 and R8, and/or R9 and R10 may together form oxo; a method for inducing a callus and a method for plant transformation using such agent for inducing a callus.Type: GrantFiled: May 28, 2015Date of Patent: June 9, 2020Assignee: RikenInventors: Takeshi Nakano, Tadao Asami, Ayumi Yamagami, Hiroyuki Osada, Misato Ohtani, Taku Demura
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Publication number: 20170105414Abstract: This invention relates to an agent for inducing a callus comprising a compound represented by Formula (I) or a hydrolysis product of an amide bond thereof: wherein Ar1 represents phenyl substituted with substituent or substituents selected from alkoxy and methylenedioxy; Ar2 represents phenyl substituted with halogen; R1 and R2 each represent hydrogen, alkyl, cyano, or carboxyl; R1 and R2 may together form oxo; R3 to R10 each represent hydrogen or methyl; and R3 and R4, R5 and R6, R7 and R8, and/or R9 and R10 may together form oxo; a method for inducing a callus and a method for plant transformation using such agent for inducing a callus.Type: ApplicationFiled: May 28, 2015Publication date: April 20, 2017Applicant: RIKENInventors: Takeshi Nakano, Tadao ASAMI, Ayumi YAMAGAMI, Hiroyuki OSADA, Misato OHTANI, Taku DEMURA
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Publication number: 20150218569Abstract: The object of the present invention is to provide a method of introducing a nucleic acid into plant cells, which is simple and widely applicable to various types of plant cells and nucleic acids. The present invention relates to a method of introducing a nucleic acid into a target plant cell, comprising a step of forming a complex by bringing a carrier peptide, wherein the carrier peptide comprises a cell-penetrating sequence and a polycationic sequence, into contact with a nucleic acid and a step of bringing the obtained complex into contact with a target plant cell.Type: ApplicationFiled: February 27, 2013Publication date: August 6, 2015Applicant: RIKENInventors: Keiji Numata, Takeshi Yoshizumi, Yutaka Kodama, Misato Ohtani, Taku Demura
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Patent number: 8878005Abstract: Provided is a method for increasing the thickness of the secondary cell wall of a plant. A method for increasing the thickness of the secondary cell wall of a plant by using a tracheary element differentiation (TED)-associated protein or a C-terminal fragment of the same, which comprises constructing a transgenic (Tg) plant containing, in an expressible manner, a DNA encoding protein(s) selected from the group consisting of a combination of TED6 with TED7, a C-terminal fragment of TED6, a C-terminal fragment of TED7, and a combination of a C-terminal fragment of TED6 with a C-terminal fragment of TED7, and expressing said DNA in said plant; the Tg plant; and a progeny, a cell, a tissue or a seed of the same.Type: GrantFiled: March 31, 2010Date of Patent: November 4, 2014Assignee: RikenInventors: Taku Demura, Satoshi Endo, Shigeru Sato, Nobuyuki Nishikubo
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Publication number: 20120110699Abstract: Provided is a method for increasing the thickness of the secondary cell wall of a plant. A method for increasing the thickness of the secondary cell wall of a plant by using a tracheary element differentiation (TED)-associated protein or a C-terminal fragment of the same, which comprises constructing a transgenic (Tg) plant containing, in an expressible manner, a DNA encoding protein(s) selected from the group consisting of a combination of TED6 with TED7, a C-terminal fragment of TED6, a C-terminal fragment of TED7, and a combination of a C-terminal fragment of TED6 with a C-terminal fragment of TED7, and expressing said DNA in said plant; the Tg plant; and a progeny, a cell, a tissue or a seed of the same.Type: ApplicationFiled: March 31, 2010Publication date: May 3, 2012Applicant: RikenInventors: Taku Demura, Satoshi Endo, Shigeru Sato, Nobuyuki Nishikubo
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Publication number: 20090004739Abstract: Conventional techniques for preparing a chimera gene having an inverted repeat sequence of a target sequence suffer from complications since a target sequence is inserted into 2 sites on a vector in sense and antisense orientations, and restriction enzyme recognition sequences must be independently provided at an insertion site of a vector and both ends of the target sequence. In this invention, a cassette construct comprising an arbitrary adaptor sequence and an inverted adaptor sequence separated by an arbitrary spacer sequence or a plasmid vector comprising such cassette construct incorporated therein is prepared, a target sequence is ligated to either or both ends of the cassette construct, or a target sequence is inserted into one end of the cassette construct on the plasmid vector, followed by PCR. Thus, a chimera gene having an inverted repeat sequence is prepared.Type: ApplicationFiled: September 22, 2004Publication date: January 1, 2009Inventors: Taku Demura, Hiroo Fukuda