Patents by Inventor Taku Demura

Taku Demura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200318125
    Abstract: It is an object of the present invention to provide a method for introducing a protein into a plant, which is simple and extensively applicable to various types of plant cells and proteins. The above object is achieved by the present invention to provide a complex comprising a protein of interest to be introduced into a target plant cell and a carrier peptide, a method for introducing a protein of interest into a target plant cell using the complex, and a kit comprising a protein of interest to be introduced into a target plant cell and a carrier peptide.
    Type: Application
    Filed: January 19, 2017
    Publication date: October 8, 2020
    Applicant: RIKEN
    Inventors: Keiji NUMATA, Yoko HORII, Takeshi YOSHIZUMI, Taku DEMURA, Yutaka KODAMA, Takashi YAMAMOTO, Tetsushi SAKUMA, Kenji MIURA, Hiroshi EZURA
  • Publication number: 20200255797
    Abstract: This invention relates to an agent for inducing a callus comprising a compound represented by Formula (I) or a hydrolysis product of an amide bond thereof: wherein Ar1 represents phenyl substituted with substituent or substituents selected from alkoxy and methylenedioxy; Ar2 represents phenyl substituted with halogen; R1 and R2 each represent hydrogen, alkyl, cyano, or carboxyl; R1 and R2 may together form oxo; R3 to R10 each represent hydrogen or methyl; and R3 and R4, R5 and R6, R7 and R8, and/or R9 and R10 may together form oxo; a method for inducing a callus and a method for plant transformation using such agent for inducing a callus.
    Type: Application
    Filed: April 28, 2020
    Publication date: August 13, 2020
    Applicant: RIKEN
    Inventors: Takeshi Nakano, Tadao ASAMI, Ayumi YAMAGAMI, Hiroyuki OSADA, Misato OHTANI, Taku DEMURA
  • Patent number: 10723806
    Abstract: The object of the present invention is to provide a method of introducing a nucleic acid into plant cells, which is simple and widely applicable to various types of plant cells and nucleic acids. The present invention relates to a method of introducing a nucleic acid into a target plant cell, comprising a step of forming a complex by bringing a carrier peptide, wherein the carrier peptide comprises a cell-penetrating sequence and a polycationic sequence, into contact with a nucleic acid and a step of bringing the obtained complex into contact with a target plant cell.
    Type: Grant
    Filed: February 27, 2013
    Date of Patent: July 28, 2020
    Assignee: Riken
    Inventors: Keiji Numata, Takeshi Yoshizumi, Yutaka Kodama, Misato Ohtani, Taku Demura
  • Patent number: 10676711
    Abstract: This invention relates to an agent for inducing a callus comprising a compound represented by Formula (I) or a hydrolysis product of an amide bond thereof: wherein Ar1 represents phenyl substituted with substituent or substituents selected from alkoxy and methylenedioxy; Ar2 represents phenyl substituted with halogen; R1 and R2 each represent hydrogen, alkyl, cyano, or carboxyl; R1 and R2 may together form oxo; R3 to R10 each represent hydrogen or methyl; and R3 and R4, R5 and R6, R7 and R8, and/or R9 and R10 may together form oxo; a method for inducing a callus and a method for plant transformation using such agent for inducing a callus.
    Type: Grant
    Filed: May 28, 2015
    Date of Patent: June 9, 2020
    Assignee: Riken
    Inventors: Takeshi Nakano, Tadao Asami, Ayumi Yamagami, Hiroyuki Osada, Misato Ohtani, Taku Demura
  • Publication number: 20170105414
    Abstract: This invention relates to an agent for inducing a callus comprising a compound represented by Formula (I) or a hydrolysis product of an amide bond thereof: wherein Ar1 represents phenyl substituted with substituent or substituents selected from alkoxy and methylenedioxy; Ar2 represents phenyl substituted with halogen; R1 and R2 each represent hydrogen, alkyl, cyano, or carboxyl; R1 and R2 may together form oxo; R3 to R10 each represent hydrogen or methyl; and R3 and R4, R5 and R6, R7 and R8, and/or R9 and R10 may together form oxo; a method for inducing a callus and a method for plant transformation using such agent for inducing a callus.
    Type: Application
    Filed: May 28, 2015
    Publication date: April 20, 2017
    Applicant: RIKEN
    Inventors: Takeshi Nakano, Tadao ASAMI, Ayumi YAMAGAMI, Hiroyuki OSADA, Misato OHTANI, Taku DEMURA
  • Publication number: 20150218569
    Abstract: The object of the present invention is to provide a method of introducing a nucleic acid into plant cells, which is simple and widely applicable to various types of plant cells and nucleic acids. The present invention relates to a method of introducing a nucleic acid into a target plant cell, comprising a step of forming a complex by bringing a carrier peptide, wherein the carrier peptide comprises a cell-penetrating sequence and a polycationic sequence, into contact with a nucleic acid and a step of bringing the obtained complex into contact with a target plant cell.
    Type: Application
    Filed: February 27, 2013
    Publication date: August 6, 2015
    Applicant: RIKEN
    Inventors: Keiji Numata, Takeshi Yoshizumi, Yutaka Kodama, Misato Ohtani, Taku Demura
  • Patent number: 8878005
    Abstract: Provided is a method for increasing the thickness of the secondary cell wall of a plant. A method for increasing the thickness of the secondary cell wall of a plant by using a tracheary element differentiation (TED)-associated protein or a C-terminal fragment of the same, which comprises constructing a transgenic (Tg) plant containing, in an expressible manner, a DNA encoding protein(s) selected from the group consisting of a combination of TED6 with TED7, a C-terminal fragment of TED6, a C-terminal fragment of TED7, and a combination of a C-terminal fragment of TED6 with a C-terminal fragment of TED7, and expressing said DNA in said plant; the Tg plant; and a progeny, a cell, a tissue or a seed of the same.
    Type: Grant
    Filed: March 31, 2010
    Date of Patent: November 4, 2014
    Assignee: Riken
    Inventors: Taku Demura, Satoshi Endo, Shigeru Sato, Nobuyuki Nishikubo
  • Publication number: 20120110699
    Abstract: Provided is a method for increasing the thickness of the secondary cell wall of a plant. A method for increasing the thickness of the secondary cell wall of a plant by using a tracheary element differentiation (TED)-associated protein or a C-terminal fragment of the same, which comprises constructing a transgenic (Tg) plant containing, in an expressible manner, a DNA encoding protein(s) selected from the group consisting of a combination of TED6 with TED7, a C-terminal fragment of TED6, a C-terminal fragment of TED7, and a combination of a C-terminal fragment of TED6 with a C-terminal fragment of TED7, and expressing said DNA in said plant; the Tg plant; and a progeny, a cell, a tissue or a seed of the same.
    Type: Application
    Filed: March 31, 2010
    Publication date: May 3, 2012
    Applicant: Riken
    Inventors: Taku Demura, Satoshi Endo, Shigeru Sato, Nobuyuki Nishikubo
  • Publication number: 20090004739
    Abstract: Conventional techniques for preparing a chimera gene having an inverted repeat sequence of a target sequence suffer from complications since a target sequence is inserted into 2 sites on a vector in sense and antisense orientations, and restriction enzyme recognition sequences must be independently provided at an insertion site of a vector and both ends of the target sequence. In this invention, a cassette construct comprising an arbitrary adaptor sequence and an inverted adaptor sequence separated by an arbitrary spacer sequence or a plasmid vector comprising such cassette construct incorporated therein is prepared, a target sequence is ligated to either or both ends of the cassette construct, or a target sequence is inserted into one end of the cassette construct on the plasmid vector, followed by PCR. Thus, a chimera gene having an inverted repeat sequence is prepared.
    Type: Application
    Filed: September 22, 2004
    Publication date: January 1, 2009
    Inventors: Taku Demura, Hiroo Fukuda