Abstract: A gut organoid includes a tissue structure that encloses a cavity. A method of using the gut organoid includes providing a test drug to the gut organoid in the presence of a buffer solution so that liquid comprising the test drug enters into the cavity, and collecting and analyzing the liquid in the cavity. A method for producing the gut organoid includes plating cells on a cell culture substrate including a surface on which a cell-adhesive region and a non-cell-adhesive region surrounding the cell-adhesive region are formed, and culturing the cells plated on the cell culture substrate so as to induce differentiation of the cells.

Abstract: A gut organoid includes a tissue structure that encloses a cavity. A method of using the gut organoid includes providing a test drug to the gut organoid in the presence of a buffer solution so that liquid comprising the test drug enters into the cavity, and collecting and analyzing the liquid in the cavity. A method for producing the gut organoid includes plating cells on a cell culture substrate including a surface on which a cell-adhesive region and a non-cell-adhesive region surrounding the cell-adhesive region are formed, and culturing the cells plated on the cell culture substrate so as to induce differentiation of the cells.

Abstract: A gut organoid includes a tissue structure that encloses a cavity. A method of using the gut organoid includes providing a test drug to the gut organoid in the presence of a buffer solution so that liquid comprising the test drug enters into the cavity, and collecting and analyzing the liquid in the cavity. A method for producing the gut organoid includes plating cells on a cell culture substrate including a surface on which a cell-adhesive region and a non-cell-adhesive region surrounding the cell-adhesive region are formed, and culturing the cells plated on the cell culture substrate so as to induce differentiation of the cells.

Abstract: A cavity vibrates a sample and is combined with the sample to be equivalent to an LC circuit. An insulator electrically insulates the sample from the cavity. The insulator is placed between the sample and the cavity. A first output unit outputs, to the cavity, a first frequency signal including a frequency which causes the cavity combined with the sample to function as an equivalent circuit of an LC circuit. A second output unit outputs, to the sample and the cavity, a second frequency signal including a frequency which vibrates the sample. An amplitude detection unit detects, based on the second frequency signal, the amplitude of a reflected signal reflected from the cavity, the reflected signal being caused by the first frequency signal.

Abstract: A carcass rubber composition for tires comprising a polymer blend of diene synthetic elastomer and natural rubber. Said diene synthetic elastomer contains a solution polymerization star shaped SBR, preferably, in the ratio of 20-50 percent. 1, 2 structure in the butadiene unit is preferably less than 50 percent. The styrene content is preferably 10-20 percent.

Abstract: An automotive tire tread having a low rolling resistance including a cap tread rubber and a base tread rubber wherein the value of the loss tangent of the cap tread rubber is not more than 0.24 and the value of the resilience of the cap tread rubber is more than 60%. In addition, the value of the loss tangent of the base tread rubber should be less than that of the cap tread rubber.

Abstract: A rubber composition for a side wall of tires comprising a polymer blend of 20-100 parts by weight of solution polymerization star shaped SBR and 80-0 parts by weight of natural rubber and/or diene synthetic rubber. The glass transition temperature (Tg) of said star shaped SBR is preferably less than -50.degree. C. Styrene content in said star shaped SBR is preferably less than 20% parts by weight.

Abstract: A pneumatic radial-ply tire having a tread constructed of at least two kinds of rubber which is capable of attaining both braking performance and rolling resistance. The tread portion is constructed so that the tread center zone is comprised of a rubber having a low hysteresis loss and the tread side zones adjacent to the center zone on both sides thereof or the outer layers of them are each comprised of a rubber having a high hysteresis loss; the tread center zone and the tread side zones are disposed in parallel to each other in the circumferential direction or the tread side zones are each disposed adjoining to the tread center zone and in a superposing manner.