Abstract: Systems and methods for diagnosis of Acute Otitis Media (AOM) with a monopolar probe in a territory innervated by a chorda tympani nerve (CTN) by applying a stimulus by the monopolar, measuring a response to the stimulus, changing the strength of the stimulus applied based on the measured response until the measured response reaches a threshold value, determining a first stimulus strength at the threshold value, repeating these steps for another predefined location in a territory innervated by the CTN associated with a second ear, to determine a second stimulus strength at the threshold value for the second predefined location; and diagnosing AOM based on a difference between the determined first and second stimulus strengths for the first and second locations.